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MDACC RPPA-Functional Proteomics

Overview of Services

 Reverse phase protein array (RPPA) is a high-throughput antibody-based technique developed for Functional Proteomics studies to evaluate protein activities in signaling networks. The RPPA Core provides services to perform such an assay for your studies.

 

What We Do

  • Perform reverse phase protein arrays on the samples received from customers.
  • Perform custom antibody validation using a reverse phase protein array.
  • Provide standard operating procedures needed for the extraction of proteins from cell lines and tumor tissue for use in the reverse phase protein array studies.
  • Perform primary data analysis using custom software to determine signal intensity, curve construction, and relative protein expression and modification.
  • Perform protein extraction from tumor tissue or cell pellets, upon request (with additional charge), for use in the reverse phase protein array studies (see pricing below).
  • Provide updated lists of antibodies for reverse phase protein array studies:
    • Standard Panel containing ≥290 antibodies (for human samples and mouse cell lines) and ≥225 antibodies (for mouse tissue samples)
  • Provide robust information using a panel of antibodies.
  • Provide a turnaround time within 8-10 weeks of sample submission. However, occasionally, based on workload or equipment failure, analysis may take up to 12 weeks.

Leadership

Our Staff Director is Yiling Lu, M.D., who has worked on the RPPA platform since 2004 and has directed the Reverse Phase Protein Array - Functional Proteomics Core Facility since 2008.

Location and hours of operation

Located at the Zayed Building, near Main Campus

Room Z4.2040
6565 MD Anderson Blvd.
Houston, TX 77030

713.792.5743

9 a.m. - 5 p.m. Monday-Friday

 

Need Assistance with iLab?

For questions regarding your iLab account, please contact iLab support at support@ilabsolutions.com.

 

   
   

Links and Resources

Click here to visit the core website for more detailed information about services, procedures, and protocols.

Contacts

Name Role Phone Email Location
Doris Siwak
Senior Research Scientist
 
832-750-0145
 
ccsgrppa@mdanderson.org
 
Z4.3005
 
Yiling Lu, M.D
Core Director
 
713-563-4218
 
yilinglu@mdanderson.org
 

 

Service Price List


Search available services:
Name Description Price
4X SDS and/or 2-Me Addition

For use only if our core needs to add 4X SDS and/or 2-Me to partially prepared protein lysates.

  • Price is per sample
Inquire
Additional Antibody Validation--New to Core

For use if you want our core to validate a new antibody that 1) does not overlap the Expanded Antibody List and 2) has not been previously validated by our core.

  • Price is per antibody and includes a validation report and probing your samples with the antibody if it passes our standard RPPA validation process
  • At least 50 uL of the antibody must be supplied to our core for validating and probing.
Internal $750.00 each
External in network $750.00 each
External out of network $1,215.00 each
UT-System $750.00 each
Non-academic $1,267.50 each
Additional Antibody Probing--Previously Validated by Core

For use if you want our core to probe your samples with an additional antibody the has been previously validated by our core.

  • Price is per antibody
  • At least 50 uL of the antibody must be supplied to our core for probing.
Internal $300.00 each
External in network $300.00 each
External out of network $486.00 each
UT-System $300.00 each
Non-academic $507.00 each
Additional Data Sets and/or Heatmaps

For use only if you are requesting additional or changes in data sets and/or heatmaps after receiving initial RPPA report.

  • Price is per data set/heatmap and includes a report containing raw, normalized, and median-centered data and two heatmaps (1 supervised and 1 unsupervised)
Inquire
Lysis and 4X SDS Buffers

For use only if you are requesting pickup or shipment of buffers from our core before sample submission. 

  • Price is per milliliter
  • Lysis - Store at -20 until use.  Lysis buffer contains all necessary inhibitors and is ready to use.  
  • 4X SDS - Store at room temperature until use.  Add 2-Me immediately before use and discard any leftover solution.
Inquire
Protein Extraction

Our core extracts protein from dry flash frozen cell pellets and fresh flash frozen tissues.

  • Price is per sample and includes addition of 4X SDS and 2-Me
  • Cell Pellets - require approximately 1-5 million cells per sample
  • Tissues - require a piece of tissue that weighs approximately 15-20 mg per sample
Inquire
RPPA Processing
  • Price is per sample and includes raw, normalized, and median-centered data and two heatmaps (1 supervised and 1 unsupervised) for antibodies on the Expanded Antibody List
Inquire
Return Residual Material
  • Price is per sample set
Internal $250.00 each
External in network $250.00 each
External out of network $405.00 each
UT-System $250.00 each
Non-academic $422.50 each