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MDACC RPPA-Functional Proteomics

Overview of Services

 Reverse phase protein array (RPPA) is a high-throughput antibody-based technique developed for Functional Proteomics studies to evaluate protein activities in signaling networks. The RPPA Core provides services to perform such an assay for your studies.

 

What We Do

  • Perform reverse phase protein arrays on the samples received from customers.
  • Perform custom antibody validation using a reverse phase protein array.
  • Provide standard operating procedures needed for the extraction of proteins from cell lines and tumor tissue for use in the reverse phase protein array studies.
  • Perform primary data analysis using custom software to determine signal intensity, curve construction, and relative protein expression and modification.
  • Perform protein extraction from tumor tissue or cell pellets, upon request (with additional charge), for use in the reverse phase protein array studies (see pricing below).
  • Provide updated lists of antibodies for reverse phase protein array studies:
    • Standard Panel containing ≥290 antibodies (for human samples and mouse cell lines) and ≥225 antibodies (for mouse tissue samples)
  • Provide robust information using a panel of antibodies.
  • Provide a turnaround time within 8-10 weeks of sample submission. However, occasionally, based on workload or equipment failure, analysis may take up to 12 weeks.

Leadership

Our Staff Director is Yiling Lu, M.D., who has worked on the RPPA platform since 2004 and has directed the Reverse Phase Protein Array - Functional Proteomics Core Facility since 2008.

Location and hours of operation

Located at the Zayad Building, near North Campus

Room Z4.2040
6565 MD Anderson Blvd.
Houston, TX 77030

713.792.5743

9 a.m. - 4 p.m. Monday-Friday

 

Need Assistance with iLab?

For questions regarding your iLab account, please contact iLab support at support@ilabsolutions.com.

 

   
   

Links and Resources

Click here to visit the core website for more detailed information about services, procedures, and protocols.

Contacts

Name Role Phone Email Location
Kathryn Aziz
Coordinator, Research Data
 
713-792-5743
 
ccsgrppa@mdanderson.org
 
Z4.2040
 
Yiling Lu, M.D
Core Director
 

 

 

 

Service Price List


Search available services:
Name Description Price
Additional Antibody Charge and Validation--New to Core (Antibody Supplied by Customer)

For use if you would like to supply and include an antibody that is not on our Standard Antibody List and that we have not previously validated and used at our RPPA Core. 

 

*This includes validating your antibody using our standard RPPA validation process.  We will report to you whether or not your antibody passes our validation criteria and will apply your antibody to your samples upon validation.

 

Inquire
Additional Antibody Charge--Previously Validated (Antibody Supplied by Customer)

For use if you would like to supply and include an antibody that is not on our current Standard Antibody List and we have previously validated and used at our RPPA Core.

Inquire
Additional Data Sets with Heatmaps

Price is per data set, which includes a report containing raw data, normalized data, and centered-median data and a pair of heatmaps (1 supervised and 1 unsupervised).

Inquire
Protein Extraction from tissue or cell pellets

Price is per sample.

Inquire
Return Residual Material Inquire
Reverse Phase Protein Array

Price is per sample.

Inquire


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