MDACC RPPA-Functional Proteomics

Overview of Services

 Reverse phase protein array (RPPA) is a high-throughput antibody-based technique developed for Functional Proteomics studies to evaluate protein activities in signaling networks. The RPPA Core provides services to perform such an assay for your studies.

 

What We Do

  • Perform reverse phase protein arrays on the samples received from customers.
  • Perform custom antibody validation using a reverse phase protein array.
  • Provide standard operating procedures needed for the extraction of proteins from cell lines and tumor tissue for use in the reverse phase protein array studies.
  • Perform primary data analysis using custom software to determine signal intensity, curve construction, and relative protein expression and modification.
  • Perform protein extraction from tumor tissue or cell pellets, upon request (with additional charge), for use in the reverse phase protein array studies (see pricing below).
  • Provide updated lists of antibodies for reverse phase protein array studies:
    • Standard Panel containing ≥290 antibodies (for human samples and mouse cell lines) and ≥225 antibodies (for mouse tissue samples)
  • Provide robust information using a panel of antibodies.
  • Provide a turnaround time within 8-10 weeks of sample submission. However, occasionally, based on workload or equipment failure, analysis may take up to 12 weeks.

Leadership

Our Staff Director is Yiling Lu, M.D., who has worked on the RPPA platform since 2004 and has directed the Reverse Phase Protein Array - Functional Proteomics Core Facility since 2008.

Location and hours of operation

Located at the Zayad Building, near North Campus

Room Z4.2040
6565 MD Anderson Blvd.
Houston, TX 77030

713.792.5743

9 a.m. - 4 p.m. Monday-Friday

 

Need Assistance with iLab?

For questions regarding your iLab account, please contact iLab support at support@ilabsolutions.com.

 

   
   

Links and Resources

Click here to visit the core website for more detailed information about services, procedures, and protocols.

Contacts

Name Role Phone Email Location
Kathryn Aziz
Coordinator, Research Data
 
713-792-5743
 
ccsgrppa@mdanderson.org
 
Z4.2040
 
Yiling Lu, M.D
Core Director
 

 

 

 

Services

Name Description
RPPA Sample Submission Request 

Please click "Initiate Request" on the right side to begin your RPPA request

 
RPPA Service Agreement Contract Request  

For CONTRACT AGREEMENTS only with the RPPA Core (Not normally used by academic institutions)

 
Reverse Phase Protein Array 

Price is per sample.

 
Protein Extraction from tissue or cell pellets 

Price is per sample.

 
Return Residual Material   
Additional Antibody Charge (Supplied by Investigator) <b>New to core</b> 

If you would like to supply an antibody that is not on our Standard Antibody List  and  we HAVE NEVER RUN this antibody through our RPPA Core.

 

 
Additional Antibody Charge (Supplied by Investigator) Previously run though core 

For use if you would like to supply an antibody that is not on our Standard Antibody List  and  we HAVE RUN/VALIDATED this antibody through our RPPA Core.

 
Antibody Validation 

We can process your antibody request through our standard validation platform, including antibody probing on our Cell Line Superslide and Western Blotting on selected cell line lysates upon quality control procedures. Once the antibody passes our validation criteria, we will apply this antibody to the RPPA slide containing your samples. We will report to you if this particular antibody passes our RPPA validation process.

 
Data Analysis Fee with Heatmaps 

Fee is per dataset which includes a report containing raw data, normalized data, centered-median data and a pair of heatmaps (1 supervised and 1 unsupervised).

 

Service Price List


Search available services:
Name Description Price
Additional Antibody Charge (Supplied by Investigator) Previously run though core

For use if you would like to supply an antibody that is not on our Standard Antibody List  and  we HAVE RUN/VALIDATED this antibody through our RPPA Core.

Inquire
Additional Antibody Charge (Supplied by Investigator) New to core

If you would like to supply an antibody that is not on our Standard Antibody List  and  we HAVE NEVER RUN this antibody through our RPPA Core.

 

Inquire
Antibody Validation

We can process your antibody request through our standard validation platform, including antibody probing on our Cell Line Superslide and Western Blotting on selected cell line lysates upon quality control procedures. Once the antibody passes our validation criteria, we will apply this antibody to the RPPA slide containing your samples. We will report to you if this particular antibody passes our RPPA validation process.

Inquire
Data Analysis Fee with Heatmaps

Fee is per dataset which includes a report containing raw data, normalized data, centered-median data and a pair of heatmaps (1 supervised and 1 unsupervised).

Inquire
Protein Extraction from tissue or cell pellets

Price is per sample.

Inquire
Return Residual Material Inquire
Reverse Phase Protein Array

Price is per sample.

Inquire


Members at UT Proteomics

University of Texas Medical Branch - Galveston

Core Name Primary Contact Email Phone Number/Ext
Biomolecular Resource Facility

William Russell, Ph.D bill.russell@utmb.edu (409) 772-3579
Core Name Primary Contact Email Phone Number/Ext
Mass Spectrometry Laboratory

Susan Weintraub, Ph.D. weintraub@uthscsa.edu (210) 567-4043
Core Name Primary Contact Email Phone Number/Ext
MDACC NORTH Campus Flow Cytometry and Cellular Imaging Core Facility

The Flow Cytometry and Cellular Imaging Facility was established to provide access to state of the art cell analysis technology for researchers, and provides cell sorting, analytical flow cytometry, cellular imaging and custom monoclonal antibody mAb conjugations to its users.

Joan Hoover-Zuniga jezuniga@mdanderson.org 713-745-1749
MDACC Proteomics and Metabolomics

The mission of the Proteomics and Metabolomics Core Facility is to provide the research community with cutting edge expertise and services for sensitive, precise, and accurate measurement of proteins and metabolites using mass spectrometrybased technologies.p</p>

David Hawke, Ph.D dhawke@mdanderson.org 713-834-6096
MDACC RPPA-Functional Proteomics

Functional Proteomics Reverse Phase Protein Array RPPA Core provides cancer center members with access to a powerful, highthroughput, quantitative costeffective antibodybased assay to characterize basal or ligandinduced protein expression and modification, and timeresolved responses appropriat

Kathryn Aziz ccsgrppa@mdanderson.org 713-792-5743

University of Texas at Austin

Core Name Primary Contact Email Phone Number/Ext
Core Name Primary Contact Email Phone Number/Ext
UTHealth Proteomics Service Center

The CTPC services provided are designed to identify and quantitate proteins and their modifications in a broad range of research specimens from simple purified protein samples to biomarker discovery and verification in complex mixtures such as cell and tissue extracts, plasma andor other biofluids

Li Li Li.Li@uth.tmc.edu 713-500-2456