ALERT
As of November 3rd the Microarray services of our site are live and available for request!
The mission of the Sequencing and Microarray Facility is to support genomics research at MDACC by providing investigators with access to state-of-the-art instrumentation and a high level of technical expertise in a centralized facility, thereby minimizing the duplication of expensive equipment, maintaining technical excellence and enhancing research collaborations. The facility’s primary focus is sequencing and microarray technologies.
Sequencing Services
Next Generation Sequencing
Sanger DNA Sequencing
Gene Resequencing
Microarray Services
The ATGC's microarray service offers a comprehensive set of integrated gene expression, genotyping/cytogenetics and methylation services on Affymetrix and Illumina platforms that facilitate studying the role of genomic alterations and transcriptional regulation of genes on human, mouse, rat and other species.
Single Cell Analysis
The Fluidigm C1™ Single-Cell Auto Prep system employs microfluidic technology to isolate, process and prepare individual cells for downstream analysis. The C1 system isolates single cells into individual reaction chambers in Fluidigm's integrated fluidic circuit (IFC).
EdgeSeq
Other SMF Services
Sample Quality Check (DNA and RNA)
Fluorescent Fragment Analysis
The ATGC provides sample collection from several MDACC campuses:
ATGC Main Lab: BSRB S15.8425 - Open Monday to Friday from 8 am to 5:30pm
Main Campus: Room Y8.5319 (cold room) - Sample pick-up 8:30am Monday to Friday
SCRB 2: Loading dock freezer (top shelf) - Sample pick-up 7am Monday to Friday
SCRB 4: Freezer located next to the vending machine (near 4SCR1.1139) - Sample pick-up 7am Monday to Friday
Funding
This facility is funded by NCI Grant CA016672 (SMF) for Sequencing and Microarray Facility.
Core Grant Citation
Publications generated using data produced by the ATGC should cite the Core grant CA016672 (ATGC) in the acknowledgment section. Two copies of the publication should be submitted to the facility at 1515 Holcombe Boulevard, Unit 1010, Houston, TX, 77030.
Correction Requests
Please report any errors or difficulties reading this site to Erika Thompson or Sybil Varghese.
Billing Contact
Sybil Varghese, M.B.A.
Sr. Financial Analyst Department of Genetics
Room S16.8316
Phone: 713-834-6383
Email: svarghe@mdanderson.org
Vicki Huff, Ph.D: Professor of Genetics, Director
Phone: 713-834-6384
Email: vhuff@mdanderson.org
Erika Thompson, MS: Assistant Director, SMF Operational Manager
Phone: 713-834-6381
Email: ejthomps@mdanderson.org
Sharon Dent, Ph.D.: Chair, Dept of Epigenetics and Molecular Carcinogenesis Co-Director
Nick Navin, Dept of Genetics Co-Director
Xiaoping Su, Ph.D. : Professor, Dept of Bioinformatics and Comp. Biology
| Hours | Location |
|
8:00am - 5:30pm Monday - Friday |
BSRB Room S15.8425 6767 Bertner Ave. Basic Science Research Bldg. Houston TX, 77030 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Erika Thompson, M.S. |
Assistant Director
|
713-834-6381
|
ejthomps@mdanderson.org
|
BSRB S16.8316b
|
| Viju Varghese, M.H.A. |
Lab Manager
|
713-834-6197
|
vivarghes@mdanderson.org
|
BSRB S15.8425
|
| David P. Pollock |
Research Laboratory Coordinator
|
713-834-6199
|
dpollock@mdanderson.org
|
BSRB S15.8425
|
| Louis S. Ramagli, Ph.D. |
Senior Research Scientist
|
713-834-6299
|
lramagli@mdanderson.org
|
BSRB S15.8425
|
| Hongli Tang, M.D. |
Research Scientist
|
713-834-6197
|
hltang@mdanderson.org
|
BSRB S15.8425
|
| Denaha (DJ) Doss, M.S |
Research Investigator
|
713-834-6197
|
djdoss@mdanderson.org
|
BSRB S15.8425
|
| Santasri Sen, M.A |
Sr. Research Assistant
|
713-834-6197
|
santsen@mdanderson.org
|
BSRB S15.8425
|
| Marisela Mendoza M.S. |
Research Investigator
|
713-834-6199
|
mgmendoz@mdanderson.org
|
BSRB S15.8425
|
| Sybil Varghese, M.B.A. |
Sr. Financial Analyst, M.B.A.
|
713-834-6383
|
svarghe@mdanderson.org
|
BSRB S16.8316
|
| Min Zhao |
Sr. Research Assistant
|
713-834-6199
|
MZhao10@mdanderson.org
|
BSRB S15.8425
|
| Siping Sun |
Sr. Research Asisstant
|
713-834-6199
|
SSun2@mdanderson.org
|
BSRB S15.8425
|
| Services & Price List |
| ► 10X Genomics 3'mRNA Capture and Library V3 (2) | |||
| Name | Description | Price | |
|---|---|---|---|
| 1st sample | Inquire | ||
| Samples 2-8 (per sample) | Inquire | ||
| ► 10X Genomics 5' Gene expression Capture cell (2) | |||
| Name | Description | Price | |
| 1st sample | Inquire | ||
| Samples 2-8 (per sample) | Inquire | ||
| ► 10X Genomics 5' Gene expression Capture plus T and B cell (2) | |||
| Name | Description | Price | |
| 1st sample | Inquire | ||
| Samples 2-8 (per sample) | Inquire | ||
| ► 10X Genomics 5' Gene expression Capture plus T or B cell (2) | |||
| Name | Description | Price | |
| 1st sample | Inquire | ||
| Samples 2-8 (per sample) | Inquire | ||
| ► 10X Genomics ATAC-Seq (2) | |||
| Name | Description | Price | |
| 1st sample | Inquire | ||
| Samples 2-4(per sample) | Inquire | ||
| ► 10X Genomics Fixed RNA (2) | |||
| Name | Description | Price | |
| 1st sample | Inquire | ||
| Samples 2-4 (per sample) | Inquire | ||
| ► 10X Genomics Multi-ome (2) | |||
| Name | Description | Price | |
| 1st sample | Inquire | ||
| Samples 2-4 (per sample) | Inquire | ||
| ► Bioinformatics (5) | |||
| Name | Description | Price | |
| Bulk ChIP-Seq | Inquire | ||
| Bulk mRNA and Total RNA-Seq | Inquire | ||
| scRNA-Seq | Inquire | ||
| WGS (per T/N pair) | Inquire | ||
| Whole Exome | Inquire | ||
| ► Bionano (1) | |||
| Name | Description | Price | |
| Bionano-Empty Flow Cell | Inquire | ||
| ► Illumina Next Generation Sequencing- iSeq (1) | |||
| Name | Description | Price | |
| iSeq300 | Inquire | ||
| ► Illumina Next Generation Sequencing-sample preparation services- per sample (9) | |||
| Name | Description | Price | |
| Agilent library and capture -Post Capture Pooling | Inquire | ||
| cfDNA WG library preparation | Inquire | ||
| Low input mRNA-Seq | Inquire | ||
| Low input Total RNA-Seq | Inquire | ||
| NGS Custom (per hour) | Inquire | ||
| TwinStrand AML Panel (48 sample submission-price per sample) | Inquire | ||
| Twist Exome (per 8 sample capture) | Inquire | ||
| Twist RNA Exome | Inquire | ||
| Twist T200.1 | Inquire | ||
| ► Illumina Next Generation Sequencing: NovaSeq6000 (23) | |||
| Name | Description | Price | |
| S1-100 | Inquire | ||
| S1-100 Xp (lane splitting-per lane) | Inquire | ||
| S1-200 | Inquire | ||
| S1-200 Xp (lane splitting-per lane) | Inquire | ||
| S1-300 | Inquire | ||
| S1-300 Xp (lane splitting-per lane) | Inquire | ||
| S2-100 | Inquire | ||
| S2-100 Xp (lane splitting-per lane) | Inquire | ||
| S2-200 | Inquire | ||
| S2-200 Xp (lane splitting-per lane) | Inquire | ||
| S2-300 | Inquire | ||
| S2-300 Xp (lane splitting-per lane) | Inquire | ||
| S4-200 | Inquire | ||
| S4-200 Xp (lane splitting-per lane) | Inquire | ||
| S4-300 | Inquire | ||
| S4-300 Xp (lane splitting-per lane) | Inquire | ||
| SP-100 | Inquire | ||
| SP-100 Xp (lane splitting-per lane) | Inquire | ||
| SP-200 | Inquire | ||
| SP-200 Xp (lane splitting-per lane) | Inquire | ||
| SP-300 | Inquire | ||
| SP-300 Xp (lane splitting-per lane) | Inquire | ||
| SP-500 | Inquire | ||
| ► Illumina Next Generation Sequencing: NovaSeqX (7) | |||
| Name | Description | Price | |
| 1.5B-100XP (per lane) | Inquire | ||
| 1.5B-200XP (per lane) | Inquire | ||
| 1.5B-300XP (per lane) | Inquire | ||
| 10B-100XP (per lane) | Inquire | ||
| 10B-200XP (per lane) | Inquire | ||
| 10B-300XP (per lane) | Inquire | ||
| 25B-300XP (per lane) | Inquire | ||
| ► Mission Bio (2) | |||
| Name | Description | Price | |
| Targeted DNA and Protein V3 | Inquire | ||
| Targeted DNA V3 | Inquire | ||
| ► Nanopore (2) | |||
| Name | Description | Price | |
| Nanopore | Inquire | ||
| Nanopore sc cDNA | Inquire | ||
| ► Nanostring Expression Assay Processing (1) | |||
| Name | Description | Price | |
| Nanostring Processing Only (1-12 samples) | Inquire | ||
| ► NextSeq500 Sequencing- per run_High Output (3) | |||
| Name | Description | Price | |
| 150PE | Inquire | ||
| 75PE | Inquire | ||
| 75SR single read | Inquire | ||
| ► NextSeq500 Sequencing- per run_Mid Output (2) | |||
| Name | Description | Price | |
| 150PE | Inquire | ||
| 75PE | Inquire | ||
| ► Visium Spatial Transcriptomics (2) | |||
| Name | Description | Price | |
| Visium CytAssist Spatial GE FFPE (2 sample 11mm slide) | Inquire | ||
| Visium CytAssist Spatial GE FFPE (2 sample 6.5mm slide) | Inquire | ||