Welcome!
ALERT The Monoclonal Antibody Facility (MABF) provides custom monoclonal antibody production and purification to researchers at MD Anderson and beyond.
The main focus of the facility is to produce high-affinity antibodies in a high-throughput and effective manner, while concentrating on quality of product and service, as well as saving time and money for potential users.
Laura Bover, PhD
Director
Long Vien, BA
Laboratory Manager
Janis Johnson
Histology Technician
jdjohnson1@mdanderson.org
(713) 563-3203
Julio Pollarolo, BS
Research Assistant II
jpollarolo@mdanderson.org
(832) 750-1501
7455 Fannin St - South Campus Research Building
Building: SCRB 1 -Immunology Department
Floor 4
Rooms 2022 / 2158
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Laura Bover, PhD |
Director
|
713-563-3301 or 713-563-2281
|
lbover@mdanderson.org
|
SCR4.3015
|
| Long Vien |
Laboratory Manager
|
713-563-3223
|
lvien@mdanderson.org
|
SCR4.2022
|
| Service List |
| ► (22) | |||
| Name | Description | Price | |
|---|---|---|---|
| Mice provision and maintenance, immunization and serum screening | Inquire | ||
| Fusion, screening and positive selection | Inquire | ||
| Single Colony Pick-up, Screening by dilution ELISA and characterization (150 Colonies) | Inquire | ||
| Recloning/Subcloning | Inquire | ||
| Small-scale antibody purification (Protein A) - clone up to 100 ml | Inquire | ||
| Small-scale antibody purification (Protein A) - clone 101 ml to 250 ml |
|
Inquire | |
| Small-scale antibody purification (Protein A) - clone 251 ml to 500 ml | Inquire | ||
| Small-scale antibody purification (Protein A) - clone 501 ml to 1 liter | Inquire | ||
| Ascites Fluid Production | Inquire | ||
| Ascites Fluid Production / Nude Mice | Inquire | ||
| In vitro supernatant production (TC) - 501ml to 1 liter | Inquire | ||
| In vitro supernatant production (TC) - up to 100 ml | Inquire | ||
| Antibody purification (FPLC) Sample | Inquire | ||
| Antibody conjugation sample | Inquire | ||
| F(ab) and F(ab)² fragment purification | Inquire | ||
| Endotoxin test ($150 kit) Antibody | Inquire | ||
| Supernatant concentration - Antibody up to 500 ml | Inquire | ||
| Sample preparation for IHC Core (cell expansion, pellet, fixation) -Cell line | Inquire | ||
| Clone restock - Cell line hybridoma | Inquire | ||
| Other extra services (PAGE, ELISA, etc.) - Antibody | Inquire | ||
| FACS staining - Up to 20 clones | Inquire | ||
| Mycoplasma test - Cell line | Inquire | ||
| ► unclassified (15) | |||
| Name | Description | Price | |
| 100ml antibody supernatant production+antibody purification and isotyping | Inquire | ||
| ALLOY ATX-Gx Mice |
Humanized mice for immunization |
Inquire | |
| Construction of Cell Expressing the target - Vector Included - 1 Transduction | Inquire | ||
| Construction of Cell Expressing the target - Vector not Included - (1 Transduction) | Inquire | ||
| Generation of IgG antibody by Transient Transfection (with consultant) | Inquire | ||
| In vitro supernatant production. (TC)-101ml to 250ml | Inquire | ||
| invitro supernatant production (TC)-clone 251ml to 500ml | Inquire | ||
| Octet service per hour plus material (charged separately depending on the project) |
Antibody binding affinity service |
Internal
$198.00
each
|
|
| peptide analysis for immunization with peptides or DNA sequence. | Inquire | ||
| Recombinant Antibody ConstructI/ Isotype exchange/ chimera | Inquire | ||
| Sequencing Variable heavy and light chain plus comprehensive report |
Internal
$1,265.00
each
|
||
| single colony pick up ,screening by dilution ELISA and characterization (500 Colonies) | Inquire | ||
| Single Colony Pick-up, Screening by dilution ELISA and characterization (1,000 Colonies) | Inquire | ||
| Single Colony Pick-up, Screening by dilution ELISA and characterization (2,000 Colonies) | Inquire | ||
| Single Colony Pick-up, Screening by dilution ELISA and characterization (200 Colonies) | Inquire | ||
