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MDACC Monoclonal Antibody Core Facility

Overview of Services

The Monoclonal Antibody Facility (MABF) provides custom monoclonal antibody production and purification to researchers at MD Anderson and beyond.

The main focus of the facility is to produce high-affinity antibodies in a high-throughput and effective manner, while concentrating on quality of product and service, as well as saving time and money for potential users.

 

 

Leadership

 

Laura Bover

Director

 

Long Vien

Laboratory Manager

 

Janis Johnson

Research Technician III

jdjohnson1@mdanderson.org

(713) 563-3203

 

Julio Pollarolo

Research Technician III

jpollarolo@mdanderson.org

(713) 563-9928

Location

 

SCRB
Floor 4
Rooms 2022 / 2158

 

Links and Resources

  1. Department of Immunology
  2. Core Facilities and Services 
  3. Antibodypedia
  4. Antibody Directory
  5. Antibodies Online
  6. BioBrea
  7. Cite Ab
  8. Labome
  9. Linscott Directory
  10. JN Biosciences LLC (Humanization of Monoclonal Antibodies Service)

Contacts

Name Role Phone Email Location
Laura Bover
Director
 
713-563-3301 or 713-563-2281
 
lbover@mdanderson.org
 

 
Long Vien
Laboratory Manager
 
713-563-3223
 
lvien@mdanderson.org
 

 

Services

Name Description
Octet Service Request   
Service Request   
F(ab')2 or Fab generation If kit is provided   
Octet service per hour plus material (charged separately depending on the project) 

Antibody binding affinity service

 
Mice provision and maintenance, immunization and serum screening   
Fusion, screening and positive selection   
Single colony pick up, screening by dilution ELISA and characterization   
Recloning/Subcloning   
Small-scale antibody purification (Protein A) - clone up to 100 ml    
Small-scale antibody purification (Protein A) - clone 100 ml to 250 ml 

 

 

 
Small-scale antibody purification (Protein A) - clone 250 ml to 500 ml    
Small-scale antibody purification (Protein A) - clone 500 ml to 1 liter   
Ascites Fluid Production   
Ascites Fluid Production / Nude Mice   
<i>In vitro</i> supernatant production (TC) - up to a liter   
<i>In vitro</i> supernatant production (TC) - up to 100 ml   
Antibody purification (FPLC) Sample   
Antibody conjugation sample   
F(ab) and F(ab)&sup2 fragment purification   
Endotoxin test ($150 kit) Antibody   
Supernatant concentration - Antibody up to 500 ml   
Sample preparation for IHC Core (cell expansion, pellet, fixation) -Cell line   
Clone restock - Cell line hybridoma   
Other extra services (PAGE, ELISA, etc.) - Antibody   
FACS staining - Up to 20 clones   
Mycoplasma test - Cell line   

Service List


Search available services: View: by category alphabetically
(22)
unclassified (2)


Members at UT System

Core Name Primary Contact Email Phone Number/Ext
MDACC Bionutrition Research Core

Christine Ranieri, MS, RD, LD brc@mdanderson.org 713-794-1295
MDACC Biospecimen Extraction Facility

The Biospecimen Extraction Resource provides a centralized laboratory for standardized, high quality DNA, RNA and protein extraction from blood, tissues, saliva, cells, or other patient derived biospecimens.

Jian Gu, Ph.D. jiangu@mdanderson.org 713-792-8016
MDACC Bone Histomorphometry Core Laboratory

The Bone Histomorphometry Core Laboratory provides histomorphometric analyses of nondecalcified bone specimens, including processing mouse bone specimens for nondecalcified histology and performing static and dynamic histomorphometric analyses of mouse bone specimens.

Michael Starbuck mwstarbu@mdanderson.org 713-563-1212
MDACC Characterized Cell Line Core Facility

The Characterized Cell Line distributes well characterized and validated cell lines to researchers across the world and offers cell line validation using short tandem repeats STR profiling.

Xuesong Li CCSGCCLC@mdanderson.org 713-792-6833
MDACC Chemical Imaging Research Core

Dodge Baluya DLBaluya@mdanderson.org 713-794-1661
MDACC Core for Biomolecular Structure and Function (CBSF)

The purpose of the Core for Biomolecular Structure and Function (CBSF) is to provide recombinant &nbsp;protein production, protein characterization, binding studies and structural biology (X-ray crystallography)&nbsp;services to MD Anderson Cancer Center and the wider Texas Medical Center.

Paul Leonard PLeonard1@mdanderson.org 832-750-1516
MDACC DNA Methylation Core

The DNA Methylation Core is funded by the Center for Cancer Epigenetics and provides services related to the analysis of DNA methylation for researchers at the University of Texas M.D. Anderson Cancer Center.

Marcos Estecio mestecio@mdanderson.org 713-792-9108
MDACC Gene Editing/Cellular Model Core Facility

The Gene Editing Cellular Model Core Facility provides custom cellular model construction services using two technical platforms.

Dr. Junjie Chen jchen8@mdanderson.org 713-792-4863
MDACC Genetically Engineered Mouse Facility (GEMF)

The Genetically Engineered Mouse Facility provides unique models with wide application to basic and translational research. The GEMF supplies indispensable expertise to generate these models in an efficient and cost effective manner using state of the art procedures.

Jan Parker-Thornburg, Ph.D. jpthorn@mdanderson.org 713-745-2654, 713-745-1419 (lab)
MDACC High Resolution Electron Microscopy Facility (HREMF)

The mission of the High Resolution Microscopy Facility is to provide scanning and transmission electron microscopy services to researchers.

Robert R. Langley, Ph.D. rlangley@mdanderson.org 713-792-9142, 713-792-8747 (fax)
MDACC Laboratory Animal Genetic Services

The Laboratory Animal Genetic Service Core provides services for speed congenics, background characterization, microbial PCR testing, rodent STR and murine genetic consultations.

Fernando Benavides, DVM, PhD, DACLAM fbenavid@mdanderson.org 512-237-9343
MDACC Molecular Cytogenetics Core Facility

The primary goal of this facility is to offer conventional and molecular cytogenetic services, including species identification, karyotyping, analysis of genomic instability, fluorescence in situ hybridization and spectral karyotyping

Asha S. Multani Ph.D. amultani@mdanderson.org 713-563-1892
MDACC Monoclonal Antibody Core Facility

The Monoclonal Antibody Facility develops high quality, cost effective customized MAbs to meet the basic, translational, and clinical research needs of MD Anderson investigators.

Laura Bover lbover@mdanderson.org 713-563-3301 or 713-563-2281
MDACC NORTH Campus Flow Cytometry and Cellular Imaging Core Facility

The Flow Cytometry and Cellular Imaging Facility was established to provide access to state of the art cell analysis technology for researchers, and provides cell sorting, analytical flow cytometry, cellular imaging and custom monoclonal antibody mAb conjugations to its users.

Joan Hoover-Zuniga jezuniga@mdanderson.org 713-745-1749
MDACC Nuclear Magnetic Resonance Facility

The NMR facility is used for structure determination of compounds developed in drug discovery, pharmacology, and diagnostic imaging laboratories.

John S. McMurray, Ph.D jmcmurra@mdanderson.org 713-745-3763
MDACC PROSPR

PROSPR provides assistance to researchers with collection and assessment of Patient Reported Outcomes, behavioral data, and measures of fitness and body composition.

Susan K. Peterson, PhD, MPH speterso@mdanderson.org 713-792-8267
MDACC Pharmaceutical Chemistry Facility

The mission of the Pharmaceutical Chemistry Facility is to assist researchers with the design and synthesis of compounds for use in cancer research.

Barbara Czako Ph.D BCzako@MDAnderson.org 713-745-2050
MDACC Protein Array & Analysis Core (PAAC)

The Protein Array and Analysis Core PAAC at the University of Texas MD Anderson Cancer Center Science Park provides investigators access to innovative and cutting edge cancer research tools for the discovery and characterization of novel proteinprotein interactions.

Cari Sagum casagum@mdanderson.org 512-237-9328
MDACC Proteomics and Metabolomics

The mission of the Proteomics and Metabolomics Core Facility is to provide the research community with cutting edge expertise and services for sensitive, precise, and accurate measurement of proteins and metabolites using mass spectrometrybased technologies.p</p>

David Hawke, Ph.D dhawke@mdanderson.org 713-834-6096
MDACC RPPA-Functional Proteomics

Functional Proteomics Reverse Phase Protein Array RPPA Core provides cancer center members with access to a powerful, highthroughput, quantitative costeffective antibodybased assay to characterize basal or ligandinduced protein expression and modification, and timeresolved responses appropriat

Keri Sherman ccsgrppa@mdanderson.org 713-792-5743
MDACC Research Histology Core Laboratory

The Research Histology Core Laboratory includes specialized equipment for immunohistochemistry, fluorescent in situ hybridization FISH, laser capture microdissection, and tissue microarray construction.

Jacqueline Furr jfurr@mdanderson.org 713-792-3119, 713-563-0544 (fax)
MDACC Science Park Flow Cytometry and Cell Imaging Core

The Flow Cytometry and Cell Imaging Core Facility at Science Park provides cutting edge cell analysis resources. These include cell sorting and analytical flow cytometry to phenotype, quantify and isolate cells and confocal microscopy to capture high resolution images.p

Pamela Whitney - Lab pjwhitney@mdanderson.org 512-237-9427
MDACC Science Park Molecular Biology Core

The Molecular Biology Core Facility at Science Park provides investigators access to sophisticated and stateoftheart molecular techniques, services and instrumentation.

Jianjun Shen, Ph.D. jianshen@mdanderson.org 512-237-9558
MDACC Science Park NGS

The Science Park NGS facility is built around a user group of 30 cancer researchers at MD Anderson Science Park, UT Austin, and Texas State University San Marcos.

Jianjun Shen, Ph.D jianshen@mdanderson.org 512-237-9558
MDACC Science Park Research Histology, Pathology & Imaging Core

The Science Park Research Histology, Pathology Imaging Core provides investigators with expert imaging and experimental pathology support. Our staff consult with investigators to formulate an integrated imaging, pathology and histology protocol for each study.

Manu Sebastian, DVM, PhD, DACVP, DABT, DECLAM mmsebastian@mdanderson.org 512-237-9347
MDACC Sequencing and Microarray Facility (SMF)

The Sequencing and Microarray Facility provides essential genomic analysis services to support researchers in basic science, translational, and populationbased research programs

Erika Thompson, M.S. ejthomps@mdanderson.org 713-834-6381
MDACC Sequencing and ncRNA Program

The Sequencing and Noncoding RNA Program is one of an MDA institution supported shared genomic service resources providing not only instrumentation but also expertise in applications of next generation sequencing and microarray services to investigators

Chang-gong Liu, Ph.D. cgliu@mdanderson.org 713-792-2287
MDACC Small Animal Imaging Facility

<p>The Small Animal Imaging Facility provides access to imaging instrumentation and expertise that allows researchers to integrate powerful and cuttingedge imaging technologies into routine preclinical research with confidence that their scientific objectives will be achieved.</p>

Charles Kingsley charles.kingsley@mdanderson.org (713) 563-6709
MDACC shRNA and ORFeome Core

The shRNA and ORFeome core Facility makes the following libraries available to MD Anderson members human and murine shRNA libraries, human ORFeome collaboration and LentiORF libraries. The core not only provides center members with individual shRNA and ORF clones but also builds customized librar...

Dr. Sun, Yutong ysun2@mdanderson.org (713) 792-3677
MDACC siRNA Core Facility

The siRNA Core Facility at MD Anderson performs High Throughput and High Content siRNA screens to assist experimental cancer therapy studies. The cores ultimate goal is to develop relevant three dimensional models that represent the human body..

Core Name Primary Contact Email Phone Number/Ext
The Shimadzu Center for Advanced Analytical Chemistry Maciej Kukula kukula@uta.edu
The Shimadzu Center for Bio-Molecular Imaging Jeff Campbell jeff.campbell@uta.edu
The Shimadzu Institute NanoTechnology Research Center Nader Hozhabri nh@uta.edu
Core Name Primary Contact Email Phone Number/Ext
Kleberg Advanced Microscopy Center

KAMC focuses on the synthesis and characterization of nanomaterials for imaging, labels for bioassays, and active targeting for in vivo or in vitro diagnostics. The Core studies the interaction of nanoparticles with living cells for application in the targeted delivery of drugs, genes, and proteins.

Miguel Jose-Yacaman, Ph.D. miguel.yacaman@utsa.edu (210) 458-6954
Protein Biomarker Core

The RCMI Proteomics and Protein Biomarkers Core Facility is focused on capillary liquid chromatographymass spectrometry LCMS and tandem mass spectrometry LCMSMS to identify, characterize, and quantify proteins. The Proteomics Core develops novel methods, while the Protein Biomarkers Core applies ...

Stephan Bach, Ph.D. stephen.bach@utsa.edu (210) 458-4466
RCMI Biophotonics Core

The RCMI Biophotonics Core Facility provides cutting edge technology for the study and manipulation of biological samples using light, including longterm indepth imaging of live tissues and the technology to probe at the molecular level for proteinprotein interactions within live samples.

George Perry george.perry@utsa.edu (210) 458-4450

University of Texas Health Northeast

Core Name Primary Contact Email Phone Number/Ext
Core Name Primary Contact Email Phone Number/Ext
UTHealth Atomic Force Microscopy

The BioScope™ II microscope has been engineered to facilitate advanced life science research such as imaging, probing and manipulating biological systems at the nanometric scale. We can determine the micromechanical properties of both biological and non-biological samples.

Ana Maria Zaske, PhD Ana.M.Zaske@uth.tmc.edu 713-486-5418
UTHealth Biomedical Informatics Group (BIG)

We offer services to researchers, including access to clinical data and enhanced subject recruiting, and are involved in multiple local and national collaborations with other groups.

Susan Guerrero Susan.C.Guerrero@uth.tmc.edu 713-500-3926
UTHealth CCTS Biobank

The CCTS Biobank consists of a collection of human samples and related clinical and genomic data that are available for collaborative sharing with qualified researchers and owned by contributing investigators conducting research within the Texas Medical Center.

Jennifer Sanner, PhD, RN Jennifer.E.Sanner@uth.tmc.edu 713.500.2114
UTHealth Cellular Therapy Core

<p>The CTC is an FDA registered facility where tissues are processed to produce cells for clinical applications. It is focused on translating, validating and supporting IND applications at a preclinical level into clinical grade processes.</p>

Fabio Triolo, Ph.D. Fabio.Triolo@uth.tmc.edu 713-486-2542
UTHealth Center for Advanced Microscopy

The Center for Advanced Microscopy provides assisted or unassisted state of the art imaging capabilities including three confocal microscopes, fluorescence lifetime imaging, live animal imaging with an IVIS Lumina, flow cytometry, and a liquid handling multiwell plate reader.

Olga Chumakova, Ph.D. Olga.Chumakova@uth.tmc.edu 713-500-7495
UTHealth Flow Cytometry Service Center

The Flow Cytometry Service Center provides a broad range of assisted and unassisted flow cytometry services from basic 2 color analysis to advanced 14 parameter analysis and multi population cell sorting.

Amy Hazen, Ph.D. Amy.Hazen@uth.tmc.edu 713-500-2486
UTHealth Human Genetics Center Laboratory

Megan Grove, MS HGC_lab@uth.tmc.edu 713-500-9800
UTHealth Magnetic Resonance Imaging Core

The MR group provides advanced MRI techniques, including selective tissue imaging, magnetic resonance spectroscopic imaging, diffusion tensor imaging to neurological disorders, central nervous system trauma, and drug addiction both in humans and animal models.

Ponnada Narayana, Ph.D Ponnada.A.Narayana@uth.tmc.edu 713-500-7677
UTHealth NanoChemistry Service Center

The Nanochemistry Service Center provides provides custom synthesis, labeling, and chemical coupling of DNA thioaptamers and nanoparticles and large-scale, high-resolution 3D printing services.

David Volk, PhD david.volk@uth.tmc.edu 713-500-3611
UTHealth Proteomics Service Center

The CTPC services provided are designed to identify and quantitate proteins and their modifications in a broad range of research specimens from simple purified protein samples to biomarker discovery and verification in complex mixtures such as cell and tissue extracts, plasma andor other biofluids

Li Li Li.Li@uth.tmc.edu 713-500-2456
UTHealth Quantitative Genomics & Microarray Service Center

The Quantitative Genomics Microarray Core Lab can perform all techniques associated with a qPCR instrument, quantitative Meso Scale ELISAs and microarrays utilizing Illumina and Agilent arrays.

David Loose, Ph.D. David.S.Loose@uth.tmc.edu 713-500-7440
UTHealth Therapeutic Antibody Core

The Therapeutic Antibody Core aims to provide statewide support and service to advance lead antibodies from academic laboratories to the stage of preclinical development.

Zhiqiang An, PhD Zhiqiang.An@uth.tmc.edu 713-500-3011
UTHealth Transgenic and Stem Cells Service Center

Eva Zsigmond, PhD eva.m.zsigmond@uth.tmc.edu 713-500-2453
Core Name Primary Contact Email Phone Number/Ext
Advanced Nucleic Acids Core Facility

Brian Wickes wickes@uthscsa.edu (210) 567-3938
Bioanalytics and Single-Cell Core

Nameer Kirma kirma@uthscsa.edu (210) 562-4068
Biobanking and Genome Analysis

Teresa L. Johnson-Pais, Ph.D. paist@uthscsa.edu 210.567.6571
Center for Macromolecular Interactions

Eileen M. Lafer lafer@uthscsa.edu (210) 567-3764
Flow Cytometry Core Facility

Karla Gorena moncadak@uthscsa.edu 210-567-3911
Mass Spectrometry Laboratory

Susan Weintraub, Ph.D. weintraub@uthscsa.edu (210) 567-4043
MicroCT Core Laboratory

Roberto Fajardo fajardor@uthscsa.edu 210-567-6176
NMR / Screening

Kristin E. Cano, Ph.D. canok@uthscsa.edu 210-567-8706
Optical Imaging Facility

Exing Wang wange3@uthscsa.edu 210-562-4062
X-Ray Crystallography

John Hart pjh@biochem.uthscsa.edu 210-567-0751

University of Texas Medical Branch - Galveston

Core Name Primary Contact Email Phone Number/Ext
Biomolecular Resource Facility

William Russell, Ph.D bill.russell@utmb.edu (409) 772-3579
International Biosafety Training Center

Sharon Walters skwalter@utmb.edu 409-772-1781
Research Histopathology Core

The Research Histopathology Core is equipped with the latest available instrumentation. RHC’s instrumentation is operated by highly skilled personnel available to assist in tissue preparation, immunohistochemistry, immunofluorescence, Insitu hybridization, including assay optimization, image anal...

Gustavo Valbuena gvalbuen@utmb.edu (409) 747-0735
Core Name Primary Contact Email Phone Number/Ext
UTSW Clinical Research Unit (CRU)/Investigational Drug Service (IDS) Lisa Fleming, MPA CRUAdministration@UTSouthwestern.edu 214.648.2718
UTSW Flow Cytometry Facility David Farrar david.farrar@utsouthwestern.edu 24-648-7315
UTSW Genomics Sequencing & Microarray Core Facility Chaoying Liang or Indu Raman Chaoying.Liang@UTSouthwestern.edu or Indu.Raman@UTSouthwestern.edu 214-648- 7329 or 214-645-6071
UTSW High Throughput Screening Core Bruce Posner, Ph.D. bruce.posner@utsouthwestern.edu 214-648-0285
UTSW Metabolic Phenotyping Core Ruth Gordillo Ruth.Gordillo@UTSouthwestern.edu 214-648-3403
UTSW O'Brien Kidney Center: Animal Models Core Lauren Diana Smith LaurenD.Smith@UTSouthwestern.edu 214-648-7222
UTSW O'Brien Kidney Center: Cell Biology and Imaging Core Lauren Diana Smith LaurenD.Smith@UTSouthwestern.edu 214-648-7222
UTSW O'Brien Kidney Center: Clinical and Translational Research Core Lauren Diana Smith LaurenD.Smith@UTSouthwestern.edu 214-648-7222
UTSW O'Brien Kidney Center: Physiology Core Lauren Diana Smith LaurenD.Smith@UTSouthwestern.edu 214-648-7222
UTSW Preclinical Pharmacology Core Noelle Williams noelle.williams@utsouthwestern.edu 214-648-5072
UTSW Rodent Behavior Core Shari Birnbaum shari.birnbaum@utsouthwestern.edu 214-648-5123
UTSW Structural Biology Laboratory
UTSW Whole Brain Microscopy Facility (WBMF) Denise Ramirez, PhD denise.ramirez@utsouthwestern.edu 214-648-0203