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MDACC Functional Genomics Core

Overview of Services

The discovery of short hairpin RNAs (shRNAs) suppressing gene expression in mammalian cells enables large-scale loss-of-function screens by using genome-wide shRNA libraries. Genome scale over-expression libraries allow large-scale gain-of-function screens. The Functional Genomics Core (previously, the ShRNA and ORFeome core) makes available to the MDACC research community the human and mouse shRNA libraries, human ORFeome collaboration, LentiORF libraries (https://dharmacon.horizondiscovery.com/) and CRISPR library (TKO v3, https://www.addgene.org/pooled-library/moffat-crispr-knockout-tkov3/). The core facility can provide individual shRNA plasmids and cDNA clones for MDACC laboratories, establishing somatic gene knockout cell lines or carry out a screening experiment using the libraries. Purchase of clones or libraries through the shRNA and ORFeome core reduces costs and decreases turnaround time for researchers.

Available Libraries:

Human and mouse ShRNA libraries: GIPZ Lentiviral shRNAmir libraries targets the entire human and mouse genome with multiple shRNAmir per target gene.For details, please visit: https://dharmacon.horizondiscovery.com/rnai/shrna/gipz-lentiviral-shrna/

Human ORFeome Collaboration: each clone contains a sequence-verified open reading frame (ORF) subcloned into Gateway™ recombinational entry vector.For details, please visit: https://dharmacon.horizondiscovery.com/cdnas-and-orfs/mammalian-orfs/orfeome-collaboration/#all


Human Precision LentiORFs: an expression-ready library, lentiviral ORFs. For details, please visit: https://dharmacon.horizondiscovery.com/cdnas-and-orfs/mammalian-orfs/precision-lentiorfs/#all

Pre-Assembled Sub-Libraries and pooled libraries

  • Human Kinase shRNA sub-libraries (40 96-well plates)
  • Human Phosphatase shRNA sub-libraries (15 96-well plates)
  • Human E3 ligase (BTB) shRNA sub-library (9 96-well plates)
  • Human E3 ligase (Ring) shRNA sub-library (15 96-well plates)
  • Human E3 ligase (DDB1-like U box F box SOCS box HECT ZNF_A20) shRNA sub-library (8 96-well plates)
  • Human pooled shRNA library (Cellecta)
  • Human pooled CRISPR library TKO v3

Services**

  • Individual Clone distribution
  • Customized shRNA or ORF libraries
  • DNA purification: Facilitated by the Tecan automated workstation, the core will purify DNA in a high throughput manner.
  • Lentiviral production and infection
  • Custom shRNA and ORF subcloning

**The shRNA and ORF reagents are available to MDACC researchers ONLY due to licensing agreements. If you have any questions, please contact Dr. Yutong Sun at ysun2@mdanderson.org.

Leadership

Dr Yu, Dihua     Director

Dr. Sun, Yutong     Co-Director

Location and hours of operation

Hours Location

Monday - Friday     9:00am - 5:00pm

1515 Holcombe Blvd., Y8.5719

Houston, TX 77030

Links and Resources

Please click here to access our website.

Contacts

Name Role Phone Email Location
Dr. Dihua Yu
Director
 
(713) 792-3636
 
dyu@mdanderson.org
 
1515 Holcombe Blvd., B7.4525, Houston, TX 77030
 
Dr. Cheng, Chien-Chia
Research Scientist
 
(713) 745-0524
 
ccheng@mdanderson.org
 
1515 Holcombe Blvd., Y8.5719, Houston, TX 77030
 
Dr. Yutong Sun
co-director
 
(713)792-3677
 
ysun2@mdanderson.org
 
1515 Holcombe Blvd., Y8.5601, Houston, TX 77030
 



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Individual Clone Distribution (12)
Lentivirus Production (5)
Lentiviral Infection (2)
Subcloning (1)
Custom Sublibrary (3)
Pre-assembled sub-library (5)