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MDACC shRNA and ORFeome Core

Overview of Services

The discovery of short hairpin RNAs (shRNAs) suppressing gene expression in mammalian cells enables large-scale loss-of-function screens by using genome-wide shRNA libraries. Genome scale over-expression libraries allow large-scale gain-of-function screens. The ShRNA and ORFeome core makes available to the MDACC research community the human and mouse shRNA libraries, human ORFeome collaboration and LentiORF libraries (http://dharmacon.gelifesciences.com/openbiosystems/). The core facility can provide individual shRNA plasmids and cDNA clones for MDACC laboratories, or carry out a screening experiment using the libraries. Purchase of clones or libraries through the shRNA and ORFeome core reduces costs and decreases turnaround time for researchers.

Available Libraries:

Human and mouse ShRNA libraries: GIPZ Lentiviral shRNAmir libraries targets the entire human and mouse genome with multiple shRNAmir per target gene.For details, please visit: http://dharmacon.gelifesciences.com/rnai-and-custom-rna-synthesis/shrna/gipz-lentiviral-shrna/

Human ORFeome Collaboration: each clone contains a sequence-verified open reading frame (ORF) subcloned into Gateway™ recombinational entry vector.For details, please visit: http://dharmacon.gelifesciences.com/gene-expression-cdnas-orfs/mammalian-orfs/orfeome-collaboration-collection/

Human Precision LentiORFs: an expression-ready library, lentiviral ORFs. For details, please visit: http://dharmacon.gelifesciences.com/gene-expression-cdnas-orfs/mammalian-orfs/precision-lentiorfs/

Pre-Assembled Sub-Libraries

  • Human Kinase shRNA sub-libraries (40 96-well plates)
  • Human Phosphatase shRNA sub-libraries (15 96-well plates)
  • Human E3 ligase (BTB) shRNA sub-library (9 96-well plates)
  • Human E3 ligase (Ring) shRNA sub-library (15 96-well plates)
  • Human E3 ligase (DDB1-like U box F box SOCS box HECT ZNF_A20) shRNA sub-library (8 96-well plates)

Services**

  • Individual Clone distribution
  • Customized shRNA or ORF libraries
  • DNA purification: Facilitated by the Tecan automated workstation, the core will purify DNA in a high throughput manner.
  • Lentiviral production and infection
  • Custom shRNA and ORF subcloning

**The shRNA and ORF reagents are available to MDACC researchers ONLY due to licensing agreements. If you have any questions, please contact Dr. Yutong Sun at ysun2@mdanderson.org.

Leadership

Dr. Hung, Mien Chie     Director

Dr Yu, Dihua     Co-Director

Dr. Sun, Yutong     Co-Director

Location and hours of operation

Hours Location

Monday - Friday     9:00am - 5:00pm

1515 Holcombe Blvd., Y8.5719

Houston, TX 77030

Links and Resources

Please click here to access our website.

Contacts

Name Role Phone Email Location
Dr. Sun, Yutong
Co-director
 
(713) 792-3677
 
ysun2@mdanderson.org
 
1515 Holcombe Blvd., Y8.5601, Houston, TX 77030
 
Dr. Hung, Mien-Chie
Director
 
(713) 792-3668
 
mhung@mdanderson.org
 
1515 Holcombe Blvd., Y8.6015, Houston, TX 77030
 
Dr. Cheng, Chien-Chia
Research Scientist
 
(713) 745-0524
 
ccheng@mdanderson.org
 
1515 Holcombe Blvd., Y8.5719, Houston, TX 77030
 



Search available services: View: by category alphabetically
Individual Clone Distribution (12)
Lentivirus Production (5)
Lentiviral Infection (2)
Subcloning (1)
Custom Sublibrary (3)
Pre-assembled sub-library (5)


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