This core ended 11/30/2021. The Science Park NGS facility is built around a user group of 30 cancer researchers at MD Anderson Science Park, UT Austin, and Texas State University-San Marcos. Other qualified researchers in Central Texas can use the services of the NGS Facility, subject to availability of time on the sequencer. Over the course of the first year, we have established several sequencing protocols and their associated bioinformatics pipelines, and we continue to add new capabilities based on the research needs of our user group.
Jianjun (J-J) Shen, PhD
Director, Science Park NGS Facility
Professor, Molecular Carcinogenesis
(512) 237-9558
jianshen@mdanderson.org
Hours | Location |
By Appointment Only |
SRM 1. Room 309 |
Name | Role | Phone | Location | |
---|---|---|---|---|
Jianjun Shen, Ph.D |
Director
|
512-237-9558
|
jianshen@mdanderson.org
|
|
Sally Gaddis |
Unix Systems Administrator
|
512.237.6577
|
salgaddis@mdanderson.org
|
|
Melissa S. Simper |
Senior Research Scientist
|
512.237.9484
|
msimper@mdanderson.org
|
|
Luis Della Coletta |
Research Laboratory Manager
|
512.237.6446
|
ldcoletta@mdanderson.org
|
|
Yueping Chen |
Research Investigator
|
512.237.9555
|
yuechen@mdanderson.org
|
|
Carol Mikulec |
Research Technician III
|
512-237-9483
|
cmikulec@mdanderson.org
|
Services |
► Bioanalyzer (4) | |||
Name | Description | Price | |
---|---|---|---|
Agilent High Sensitivity DNA Kit |
per chip |
Inquire | |
Agilent RNA 6000 Nano Kit |
per chip |
Inquire | |
Agilent RNA 6000 Pico Kit |
per chip |
Inquire | |
Agilent Small RNA Kit |
per chip
|
Inquire | |
► Sample Preparation (16) | |||
Name | Description | Price | |
RNA-seq (TruSeq Stranded Total RNA) |
Per Sample ** Please contact Core staff for consultation on kit selection. |
Inquire | |
mRNA (TruSeq Stranded mRNA) |
Per Sample |
Inquire | |
Lexogen Quant-Seq 3' mRNA |
Per Sample |
Inquire | |
microRNA-seq (Illumina kit) |
Per Sample |
Inquire | |
microRNA-seq (NEB kit) |
Inquire about pricing |
Inquire | |
Ultra Low Input RNA cDNA Synthesis (Clontech SMART-Seq v4) |
Per Sample |
Inquire | |
Library Prep for Ultra Low Input RNA (Illumina NexteraXT) |
Per Sample |
Inquire | |
TruSeq RNA Access Library Prep (FFPE) |
Per Sample |
Inquire | |
ChIP-seq (KAPA Hyper kit) |
Per Sample |
Inquire | |
ChIP-seq (NEB Ultra II kit) |
Per Sample |
Inquire | |
ChIP-seq (KAPA kit) |
Per Sample |
Inquire | |
ChIP-seq (Bioo kit) |
Per Sample |
Inquire | |
Exome-seq |
Per Sample (not including capture) |
Inquire | |
Human Exome capture |
Per Capture (1-4 samples/capture) |
Inquire | |
Mouse exome capture |
Per Capture ( 1-4 samples/capture) |
Inquire | |
RRBS Library Prep (DNA Methylation Core) |
per sample |
Inquire | |
► Sequencing (14) | |||
Name | Description | Price | |
HiSeq 3000 36 or 50 bp, single read |
Per Lane |
Inquire | |
HiSeq 3000 75 bp, paired end |
Per Lane |
Inquire | |
HiSeq 3000 100 bp, paired end |
Per Lane |
Inquire | |
HiSeq 3000 150 bp, paired end |
Per Lane |
Inquire | |
NextSeq 500 High Output 150 bp, paired end |
Per Lane |
Inquire | |
NextSeq 500 High Output 75 bp, paired end |
Per Lane |
Inquire | |
NextSeq 500 High Output 75 bp, single read |
Per Lane |
Inquire | |
NextSeq 500 Mid Output 150 bp, paired end |
Per Lane |
Inquire | |
NextSeq 500 Mid Output 75 bp, paired end |
Per Lane |
Inquire | |
MISeq 150 bp, single read |
Per Lane |
Inquire | |
MISeq 150 bp, paired end |
Per Lane |
Inquire | |
MISeq 300 bp, single read |
Per Lane |
Inquire | |
MISeq 300 bp, paired end |
Per Lane |
Inquire | |
No Reagent MiSeq 150 pb, paired end | Inquire | ||
► RNA Isolation (3) | |||
Name | Description | Price | |
RNA Isolation with Column (including DNase treatment) |
per sample
|
Inquire | |
RNA Isolation with Trizol Reagent (without DNase Treatment) |
per sample |
Inquire | |
RNA Isolation/Clean-up with Column (with DNase Treatment) |
per sample |
Inquire | |
► other (7) | |||
Name | Description | Price | |
Qubit Analysis |
per sample |
Inquire | |
KAPA Library qPCR Quantification |
per sample |
Inquire | |
Ribosomal RNA Depletion (rRNA) |
per sample |
Inquire | |
Double Fragmentation |
per sample
|
Inquire | |
DNA Clean Up |
per sample |
Inquire | |
Blue Pippin Size Selection Gel Charge | Inquire | ||
Shipping Cost |
Cost is adjusted to the actual cost of shipping. |
Inquire | |
► unclassified (1) | |||
Name | Description | Price | |
Bioinformatics Request (no charge) | Inquire |