ALERT

In Observance of U.S. holiday, Thanksgiving, Agilent Cross Lab/ iLab Operations Software will be closed during U.S. hours on Thursday November 22nd and Friday November 23rd, 2018. EU and APAC support coverage will remain open during this time. We will resume U.S. support hours on Monday November 26th, 2018. For urgent matters, please add "Urgent" to the ticket/ email subject or press "1", when prompted, to escalate a call on the iLab Support phone and we will prioritize those requests first.

MDACC Proteomics and Metabolomics

Please Acknowledge

In order to continue obtaining Core support in a challenging environment, it is crucial that our users acknowledge the following grants in all publications (see following text).  Thank you!

 

This work was supported in part by Cancer Prevention Research Institute of Texas (CPRIT) grant number RP130397 and NIH grant numbers 1S10OD012304-01 and P30CA016672.

 

Overview of Services

Mission.  The mission of the Proteomics and Metabolomics Core Facility is to provide the research community with cutting-edge expertise and services for sensitive, precise, and accurate measurement of proteins and metabolites using mass spectrometry-based technologies.

 

SERVICES

 

Proteomics:

  • Protein Identification: High sensitivity (silver stain OK), up to 100 proteins or more may be indentifiable. 
  • Molecular Weight Determination: Check the MW of your peptide or molecule.  Proteins may be measured if enough clean protein is available (larger proteins are less reliable). 
  • Quantitative Protein Analysis:  Using stable isotope methods, iTRAQ, etc. For SRM/MRM methods on our tandem quadrupole MS, please inquire. Quantitative Protein Profiling using MRM, HRM or DIA (Swath-like) methods, with or without reference peptides (lists available, custom lists can be built).
  • Posttranslational Modification Analysis:  Phosphorylation, acetylation, methylation, etc. Please inquire for more detailed information.
  • LC-MS Analysis:  Size exclusion chromatography and simple LC-MS.  Please inquire for more detailed information and other chromatographic analyses available.

 


Metabolomics: 

      Targeted

  • Amino Acids (30): Absolute quantitation of the 18 proteogenic amino acids and 12 related molecules. (See list below)
  • Organic Acids (8): Absolute quantitation of 8 organic acids. (See list below)
  • Glutathione (2): Absolute quantitation of reduced (GSH) and oxidized (GSSG) glutathione.
  • Polyamines (14): Absolute quantitation of 14 polyamines. (See list below)
  • DNA Damage Metabolites (6): Absolute quantitation of 6 DNA damage metabolites. (See list below)
  • Anionic Metabolites by IC-MS (80): Relative quantitation of metabolites associated primarily with glycolysis, TCA cycle, and pentose phosphate pathway.
  • Stable Isotope Tracer Analysis: Measurement of stable isotope incorporation into targeted metabolites (assays above) downstream of a chosen tracer metabolite. Multiplexed, multi-tracer analysis is available.

 

     Non-targeted

  • Profiling (complete): Relative quantitation of metabolites from multiple fractions.
  • Lipidomic Profiling: Relative quantitation of multiple classes of lipid metabolites from nonpolar fractions.
  • Hyaluronic Acid Profiling: Relative quantitation of hyaluronic acid oligomers.
  • Tracer Analysis: Measurement of stable isotope incorporation into non-targeted metabolites downstream of a chosen tracer metabolite.  Multiplexed, multi-tracer analysis is available.

 

     Other Services

  • Redox Metabolism Assays: Relative quantitation of ROS (H2O2) using a microplate (96-well) assay.
  • Mechanism of Action Studies: Assistance with pharmacological experiments to determine an agent's mechanism of action by integrating pharmacological endpoints (e.g., cell viability) with metabolomic and other omic data.
  • Sample Preparation Assistance: Assistance with or full-service provision of sample preparation. For example, you can ship cells and treatment(s), we can work together to design an experiment, and we can conduct the entire experiment.

 

Data Analysis:

  • Data Analysis: Many of the reports generated by our facility are time-consuming to assemble; we add a data analysis fee of $250 for reports that require 1-4 h of effort. Expertise includes bioinformatics, statistics, pathway analysis, and/or assistance preparing materials for manuscripts or presentations. Many data types accepted (genomic, proteomic, metabolomic, pharmacological, etc.).
  • Drug Combination Analysis: We have developed software for conducting 3-D response surface and response envelope analyses of drug combinations for the statistical determination of synergy, additivity, or antagonism. (Please contact Phil Lorenzi to discuss)

____________________________________________________________________________________________________________________________________________________________________________________________________________________________

 AMINO ACIDS: 

1 4-hydroxy-proline 16 isoleucine (Ile)
2 acetylcholine 17 leucine (Leu)
3 alanine (Ala) 18 lysine (Lys)
4 arginine (Arg) 19 methionine (Met)
5 asparagine (Asn) 20 S-adenosylhomocysteine (SAH)
6 carnitine 21 ornithine
7 citrulline 22 phenylalanine (Phe)
8 creatine 23 proline (Pro)
9 creatinine 24 sarcosine
10 cysteine (Cys) 25 serine (Ser)
11 cystine (Cys-Cys disulfide) 26 taurine
12 gamma-aminobutyric acid (GABA) 27 threonine (Thr)
13 glutamine (Gln) 28 tryptophan (Trp)
14 glycine (Gly) 29 tyrosine (Tyr)
15 histidine (His) 30 valine (Val)

  

ORGANIC ACIDS: 

1 glutamate (Glu)
2 glutamine (Gln)
3 aspartate (Asp)
4 alpha-ketoglutarate (a-KG)
5 2-hydroxyglutarate (2-HG)
6 pyruvate
7 lactate
8 pyroglutamic acid (pGlu)

  

POLYAMINES:

1 tyramine 8 putrescine
2 tryptamine 9 agmatine
3 N-acetylputrescine 10 hypusine
4 diacetylspermine 11 diaminopropane
5 ornithine 12 N-acetylspermine
6 diaminohexane 13 spermidine
7 cadaverine 14 spermine

 

DNA DAMAGE METABOLITES:

1 NAD(+)
2 AMP
3 NADH
4 ADP-ribose
5 ADP
6 ATP


Leadership 

 

Faculty Oversight:

Dr. Mien-Chie Hung

Dr. John Weinstein

 

Proteomic Services:

David Hawke, Ph.D., Director

Email:  dhawke@mdanderson.org

713.834.6096

 

Metabolomics Services:

Phil Lorenzi, Ph.D., Co-Director

Email: PLLorenzi@mdanderson.org

713.792.9999

 

Location and hours of operation

Hours: Location:

Sample Drop Off:

Monday - Friday

7 AM - 5 PM

By appointment only!                        

Lab: 2SCR3.3217
David Hawke: 2SCR3.3025             
Phil Lorenzi: 2SCR3.3029

  

Links and Resources

Our website contains additional information including an FAQ and tutorials.

  1. Proteomics and Metabolomics Core Website

Contacts

Name Role Phone Email Location
David Hawke, Ph.D
Director
 
713-834-6096
 
dhawke@mdanderson.org
 
2SCR3.3025
 
Phil Lorenzi, Ph.D
Co-Director
 
713-792-9999
 
PLLorenzi@mdanderson.org
 
2SCR3.3029
 

Services & Rates


Search available services: View: by category alphabetically
Data Analysis (2)
Equipment Usage (2)
Metabolomics (16)
Proteomics (5)


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