The siRNA Core Facility at MD Anderson performs High Throughput and High Content siRNA screens to assist experimental cancer therapy studies. The core's ultimate goal is to develop relevant three-dimensional models that represent the human body and endeavor to find therapies effective at combating cancer.
The Core Facility offers Two-Dimensional and Three-Dimensional High Throughput Screens using the ThermoScientific Dharmacon siARRAY siRNA Collections for Gene Families Genome-Wide Library, Two-Dimensional monolayer and Three-Dimensional spheroid Drug Screens as well as embarking on CRISPR-CAS 9 High Throughput Screens with CAS-9 expressing immortalized host cells from various cancers.
All investigators interested in the services offered by the siRNA Core Facility must schedule and attend a preliminary consulatation meeting with our staff. During this meeting all essential details regarding the screen will be discussed including cell lines, assay design, standard operating procedure of a screen, timeline, cost and cell culture models.
To schedule a preliminary meeting, initiate a "Preliminary Consultation Meeting Request" form HERE.
New users must first create an iLab account HERE before they are able to access this site further or request services.
Current iLab users can login with the green button at the top right hand side of this page.
Core Director
Geoffrey Bartholomeusz, Ph.D., Associate Professor
Office: (713) 792-4158
Email: gbarthol@mdanderson.org
Lab Coordinator
LaKesla R. Iles
Email: lriles1@mdanderson.org
Hours | Location |
Monday - Friday 8 AM - 5 PM By Appointment only |
MD Anderson Cancer Center South Campus Room 4SCR4.2085A Core Facility phone number: (713) 563-2852 |
Services & Rates |
► Instrument Costs (4) | |||
Name | Description | Price | |
---|---|---|---|
Imager deck time |
Hourly rate |
Inquire | |
INCELL 6000 Usage Fee |
Fee per instrument, per project |
Inquire | |
Scanner Use Fee | Inquire | ||
Technician Time |
Hourly rate |
Inquire | |
► Screen Costs (4) | |||
Name | Description | Price | |
CRISPR/Cas9 Method 1 | Inquire | ||
CRISPR/Cas9 Method 2 | Inquire | ||
External User Screen Library Charge |
Per project to cover cost of license |
Inquire | |
gRNA Transfections | Inquire | ||
► Services (47) | |||
Name | Description | Price | |
2D and 3D morphology of parental host cell and 6 Cas9 clones | Inquire | ||
50 mL Tube of FBS | Inquire | ||
Assay development to identify optimum transfection conditions | Inquire | ||
Blasticidine | Inquire | ||
Cas9 recombinant protein | Inquire | ||
Commercially synthesizing 3x gRNA | Inquire | ||
CRISPR MAx Transfection Reagent | Inquire | ||
CRISPR Service Fee | Inquire | ||
Director Time | Inquire | ||
Expand cells into 6 x 6 well plates | Inquire | ||
Expand clones into 3 x 12 well plates | Inquire | ||
Expand single clones into 2x24 well plates | Inquire | ||
Expansion into 3 separate wells of single well of a 6 well plate | Inquire | ||
Expansion into a single well of a 6 well plate | Inquire | ||
Freeze Down | Inquire | ||
Growth curve with parental host cell and 6 Cas9 clones | Inquire | ||
Lenti Viral Particles | Inquire | ||
Lentivirus/Cas9 Infection 96 well plate | Inquire | ||
Media | Inquire | ||
Set 1 Freeze Down | Inquire | ||
Set 1 Screen by Western blot for target gene expression | Inquire | ||
Set 2 - Screen by Western blot for Cas9 expression | Inquire | ||
Set 2 - Screen by Western blot for target gene knockout | Inquire | ||
Set 2 Maintenance Set | Inquire | ||
Spilt cells into 2 sets of 6 x 6 well plates | Inquire | ||
Technician Time | Inquire | ||
Transfection of cells with 3 gRNA | Inquire | ||
Two Condition Kinome (EDGE) 2D or 3D | Inquire | ||
Consultation |
Required for project design. No cost. |
Inquire | |
Mycoplasma test |
Per cell line, each time. |
Inquire | |
Freezing Down Cells | Inquire | ||
Growth Curve | Inquire | ||
Assay Development Plates | Inquire | ||
Assay Development trasnfection reagent 8-pack (per uL) | Inquire | ||
Test plate control siRNA (per nmole) | Inquire | ||
Robotic tips to add test plate control siRNA (per box) | Inquire | ||
Print test plate consumables (per plate) | Inquire | ||
Cell culturing Supplies | Inquire | ||
Control plates printed specifically for PI's Cell line | Inquire | ||
Transfection reagent | Inquire | ||
OPTIMEM medium | Inquire | ||
Cell Titer Glo | Inquire | ||
Cell Titer Blue | Inquire | ||
Disposable 5uL tubing set for reagent dispensor | Inquire | ||
Two-Dimensional One Condition Kinome Service Fee | Inquire | ||
Two-Dimensional One Condition Druggable Service Fee | Inquire | ||
Two-Dimensional One Condition Genome Service Fee | Inquire | ||
► Core Use Only (3) | |||
Name | Description | Price | |
Grant Discount- Druggable Screen | Inquire | ||
Grant Discount- Genome Screen | Inquire | ||
Grant Discount- Kinome Screen | Inquire |