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MDACC Molecular Cytogenetics Core Facility

Overview of Services

The primary goal of this facility is to offer conventional and molecular cytogenetic services, including species identification, karyotyping, analysis of genomic instability, fluorescence in situ hybridization and spectral karyotyping. Dr. Guillermina Lozano is the Director and Dr. Asha S. Multani is the Co-Director of the facility. MCF is administered through the Department of Genetics.

 

Services

The Molecular Cytogenetics Core Facility provides the following services:

  • Study of genetic instability by chromosome analysis
  • Cell line identification: human, mouse, rat, dog, monkey or of any other mammalian origin
  • Giemsa (G), C, Q, R –banding, SCE (Sister Chromatid Exchange) and silver staining of metaphase chromosomes
  • Analysis of meiotic chromosomes of laboratory animals
  • Effects of pharmaceutical products on human and other mammalian chromosomes
  • Fluorescence in situ hybridization (FISH) on interphase and metaphase cells using whole chromosome paints, centromere probes, gene mapping with commercial and laboratory-generated DNA probes
  • Quantitative FISH (Q-FISH) analysis of telomeric sequences
  • Spectral karyotyping (SKY) in human, mouse and rat cells
  • Determination of the genetic suitability of mammalian stem cells to be used for cloning purposes

 

Equipment

The Molecular Cytogenetics Core Facility is equipped with:

  • Nikon Eclipse 800 upright microscope with two CCD ports
  • Applied Spectral Imaging SKY interferometer plus workstation
  • Sensys CCD camera for FISH image acquisition and analysis
  • PSI Genetiscan cytogenetics workstation with dedicated EM 400 Nikon Microscope, CCD camera and karyotyping software

 

Education

We will take the time to understand your project and plan for the preparation of samples for analysis. We will help with interpretation of the results and upon request provide a detailed description of method as well as microphotographs (color or black and white) suitable for inclusion in reports or manuscripts to the investigators.

 

Core Grant Citation

Publications should cite the Core in the acknowledgment section if publications use data generated by the Core facility. Two copies of the publication acknowledging the Core grant should also be submitted to the facility at 1515 Holcombe Blvd., Unit 1010, Houston, TX, 77030

 

Leadership

 

Guillermina Lozano, Ph.D., Director
Phone: 713-834-6386
E-mail: gglozano@mdanderson.org

 

Asha S. Multani, Ph.D., Co-Director
Phone: 713-563-1892
E-mail: amultani@mdanderson.org

 

Sen Pathak, Ph.D., F.N.A.Sc., Research Professor
Phone: 713-563-1892
E-mail: spathak@mdanderson.org

 

Jin Ma, Research Investigator
Phone: 713-563-1892
E-mail: jma@mdanderson.org

 

 

Location and hours of operation

Hours Location

Samples received between 10am and 12pm Monday thru Thursday 

The Molecular Cytogenetics Core Facility is located in the Yellow Zone in Y5.5812 (lab) and Y5.5815 (office).

Links and Resources

  1. Molecular Cytogenetics Core Facility

Contacts

Name Role Phone Email Location
Asha S. Multani Ph.D.
Co-Director
 
713-563-1892
 
amultani@mdanderson.org
 

 

Service List


Search available services: View: by category alphabetically
Analysis of ES cells (4)
FISH (Fluorescence in-situ hybridization) Using Commercial Probes (5)
Genomic instability (Chromosomal aberrations) (2)
Inter-species cell line contamination (2)
Karyotypeing (G-Banding) Cell Lines (2)
Karyotyping (G-banding) Blood (2)
SKY (Spectral Karyotyping) (2)
Sister Chromatid Exchange (SCE) analysis (2)
Telomere FISH (4)
Test (1)
unclassified (4)


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