Overview of Services

ACSF iLabs

Please email questions to ACSFscheduling@mdanderson.org. Thank you!

The Advanced Cytometry & Sorting Facility at South Campus (ACSF) is equipped with 5 cell sorters, 6 flow cytometry analysis systems including two Aurora Spectral flow cytometers. The lab provides flow cytometry analysis and cell sorting services.

Leadership

Karen Clise-Dwyer, PhD
ACSF Director
713-563-3322
kcdwyer@mdanderson.org
2SCR4.2020

Core Staff

Location and hours of operation

Links and Resources

1. Equipment Scheduling: Email ACSFscheduling@mdanderson.org
2. ACSF Core external website https://www.mdanderson.org/research/research-resources/core-facilities/flow-cytometry-and-cellular-imaging-core-facility.html
3. Fluorfinder link to ACSF https://app.fluorofinder.com/mdasouth
4. Flurofinder OMIPS https://fluorofinder.com/omips/ 
5. FlowJo Recorded Webinars https://www.flowjo.com/learn/webinars/recorded
6. FlowJo Tutorial https://www.flowjo.com/learn/flowjo-university/flowjo/getting-started-in-flowjo/131
7. FCS Express Recorded Webinars https://denovosoftware.com/videos/
8. Flow Cytometry Introduction Video http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html
9. Data Analysis Introduction Video http://media.invitrogen.com.edgesuite.net/tutorials/5Data_Analysis/player.html
10. Optimized Multicolor Immunofluorescence Panels (OMIPS) https://fluorofinder.com/omips/
11. FP Base Spectral Viewer https://www.fpbase.org/spectra/
12. BDSelect-Buffer Compatibility Resource https://www.cytobank.org/facselect/
13. Antibody Validation Workshop https://go.myabcam.com/antibody-basics-training-email?utm_source=Eloqua&utm_medium=Email&utm_campaign=Welcome|Awareness|2020-02-09%2019:00:00.000|N/A|GLOBAL|EN|Automated%20-%20Lifecycle&utm_content=Email%204|&mi_u=1204290
14. BD DIVA Introduction https://static.bdbiosciences.com/training/diva_basics/module1-overview/story_html5.html
15. BD DIVA-2 https://static.bdbiosciences.com/training/diva_basics/module2-datadisplay/story_html5.html
16. BD DIVA-3 https://static.bdbiosciences.com/training/diva_basics/module4-templates/story_html5.html
17. Experimental Design Guide https://myteams.mdanderson.org/depts/sctct/fclabsouth/Shared%20Documents/Experimental%20Design%20Guide.pdf
18.  ACSF EX002-HN01 V1.1 Antibody Staining Guidelines
19.  ACSF EX003-HN01 V1.0 Cell Cycle Guidelines
20.  ACSF EX004-HN01 V1.0 Apoptosis Guidelines
21.  ACSF TR001-HN01 V1.0 New User Checklist
22.  ACSF TR001-HN02 V1.1 Cell Sorting Guidelines
23.  ACSF TR001-HN03 V1.0 Data Analysis Overview
24. ACSF TR001-HN04 V1.0 Lab Safety Overview
25. Server Login Flow Core Server Login
26. Independent Analyzer Training https://myteams.mdanderson.org/depts/sctct/fclabsouth/_layouts/15/WopiFrame.aspx?sourcedoc=/depts/sctct/fclabsouth/Shared%20Documents/ACSF%20TR001-HN07%20V1.1%20Independent%20Analyzer%20Training%20Final.pptx&action=default
27. FlowTex: Professional organization that hosts an annual conference and other cytometry education-related events. Most events are free to academic attendees.
28. BD Interactive Human Cell Map https://www.bdbiosciences.com/en-us/resources/reagent-selection-tools/interactive-cell-map
29. FlowJo_Intro-Refresher+Comp_April 2023
30. FlowJo_High Dimensional Data_2023

 Online Learning Links

      1. Online Learning Links (Select link and open in a new tab) 

 BD Analyzer Training Videos

1. How to replace an empty sheath box
2. How to empty waste container
3. How to Open DIVA software
4. How to Open an Experiment
5. How to Open Any Closed Viewing Windows
6. How to run a sample
7. How to add fluorochrome to the parameter
8. How to label marker name for respective fluorochrome
9. How to adjust the voltages
10. How to create basic plots
11. How to draw a gate and label it
12. How to do auto compensation 1
13. How to do auto compensation 2
14. How to clear a partial clog
15. What to do if no events show while acquiring sample
16. How to clean the instrument after running tube samples
17. How to switch from tube mode to plate mode
18. How to switch from plate mode to tube mode
19. How to run a clean plate           

Cytek Aurora Analyzer Training Videos

1.  How to change the empty sheath and full waste box
2.  How to startup instrument and run QC
3.  How to create a new experiment, save a template and open a template
4.  How to run reference controls into library and reuse in the experiment
5.  How to clean the instrument after running sample
6.  How to create batch analysis
7.  How to export the data and transfer it to OneDrive
8.  How to shutdown the instrument (general)
9.  How to shutdown Aurora Rose
10.  How to shutdown Aurora Blue

Contacts