MDACC Advanced Cytometry & Sorting Facility at South Campus

Overview of Services

ACSF iLabs

Please email questions to ACSFscheduling@mdanderson.org. Thank you!

The Advanced Cytometry & Sorting Facility at South Campus (ACSF) is equipped with 5 cell sorters, 6 flow cytometry analysis systems including two Aurora Spectral flow cytometers. The lab provides flow cytometry analysis and cell sorting services.

 

Leadership

Karen Clise-Dwyer, PhD
ACSF Director
713-563-3322
kcdwyer@mdanderson.org
2SCR4.2020

Core Staff

Name Role Phone Email Location
Karen Clise-Dwyer Associate Professor, Director 713-563-3322 kcdwyer@mdanderson.org 2SCR4.2020
Karen Ramirez Lab Manager 713-563-3345 kmramirez@mdanderson.org 2SCR4.2120
David Dwyer Coordinator, Flow Cytometry Lab 713-563-3345 ddwyer@mdanderson.org 2SCR4.2120
Veena Papanna Coordinator, Flow Cytometry Lab 713-563-3345 vpapanna@mdanderson.org 2SCR4.2120
Kathryn Cox Coordinator, Flow Cytometry Lab 713-563-3350 kequinta@mdanderson.org 2SCR4.2213
Matthew Ramirez Student Extern 713-563-3345 msramirez1@mdanderson.org 2SCR4.2120
         
 
 
   
Hours Location

Staffed: 8 AM - 5 PM

Trained User's have access 24/7      

South Campus Research Building 2, Room 2SCR4.2120

7435 Fannin St., Houston, TX 77054

Location and hours of operation

Links and Resources

  1. Equipment Scheduling: Email ACSFscheduling@mdanderson.org
  2. ACSF Core external website https://www.mdanderson.org/research/research-resources/core-facilities/flow-cytometry-and-cellular-imaging-core-facility.html
  3. Fluorfinder link to ACSF https://app.fluorofinder.com/mdasouth
  4. FlowJo Recorded Webinars https://www.flowjo.com/learn/webinars/recorded
  5. FlowJo Tutorial https://www.flowjo.com/learn/flowjo-university/flowjo/getting-started-in-flowjo/131
  6. FCS Express Recorded Webinars https://denovosoftware.com/videos/
  7. Flow Cytometry Introduction Video http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html
  8. Data Analysis Introduction Video http://media.invitrogen.com.edgesuite.net/tutorials/5Data_Analysis/player.html
  9. Optimized Multicolor Immunofluorescence Panels (OMIPS) https://fluorofinder.com/omips/
  10. FP Base Spectral Viewer https://www.fpbase.org/spectra/
  11. BDSelect-Buffer Compatibility Resource https://www.cytobank.org/facselect/
  12. Antibody Validation Workshop https://go.myabcam.com/antibody-basics-training-email?utm_source=Eloqua&utm_medium=Email&utm_campaign=Welcome|Awareness|2020-02-09%2019:00:00.000|N/A|GLOBAL|EN|Automated%20-%20Lifecycle&utm_content=Email%204|&mi_u=1204290
  13. BD DIVA Introduction https://static.bdbiosciences.com/training/diva_basics/module1-overview/story_html5.html
  14. BD DIVA-2 https://static.bdbiosciences.com/training/diva_basics/module2-datadisplay/story_html5.html
  15. BD DIVA-3 https://static.bdbiosciences.com/training/diva_basics/module4-templates/story_html5.html
  16. Experimental Design Guide https://myteams.mdanderson.org/depts/sctct/fclabsouth/Shared%20Documents/Experimental%20Design%20Guide.pdf
  17.  ACSF EX002-HN01 V1.1 Antibody Staining Guidelines
  18.  ACSF EX003-HN01 V1.0 Cell Cycle Guidelines
  19.  ACSF EX004-HN01 V1.0 Apoptosis Guidelines
  20.  ACSF TR001-HN01 V1.0 New User Checklist
  21.  ACSF TR001-HN02 V1.1 Cell Sorting Guidelines
  22.  ACSF TR001-HN03 V1.0 Data Analysis Overview
  23. ACSF TR001-HN04 V1.0 Lab Safety Overview
  24. Server Login Flow Core Server Login
  25. Independent Analyzer Training https://myteams.mdanderson.org/depts/sctct/fclabsouth/_layouts/15/WopiFrame.aspx?sourcedoc=/depts/sctct/fclabsouth/Shared%20Documents/ACSF%20TR001-HN07%20V1.1%20Independent%20Analyzer%20Training%20Final.pptx&action=default
  26. FlowTex: Professional organization that hosts an annual conference and other cytometry education-related events. Most events are free to academic attendees.
  27. BD Interactive Human Cell Map https://www.bdbiosciences.com/en-us/resources/reagent-selection-tools/interactive-cell-map

 

 Online Learning Links

      1. Online Learning Links (Select link and open in a new tab) 

 Training Videos

  1. How to replace an empty sheath box
  2. How to empty waste container
  3. How to Open DIVA software
  4. How to Open an Experiment
  5. How to Open Any Closed Viewing Windows
  6. How to run a sample
  7. How to add fluorochrome to the parameter
  8. How to label marker name for respective fluorochrome
  9. How to adjust the voltages
  10. How to create basic plots
  11. How to draw a gate and label it
  12. How to do auto compensation 1
  13. How to do auto compensation 2
  14. How to clear a partial clog
  15. What to do if no events show while acquiring sample
  16. How to clean the instrument after running tube samples
  17. How to switch from tube mode to plate mode
  18. How to switch from plate mode to tube mode
  19. How to run a clean plate           

 

 

Contacts

Name Role Phone Email Location
Karen Clise-Dwyer
Director
 
713-563-3322
 
kcdwyer@mdanderson.org
 
2SCR4.2020