Overview of Services
ACSF iLabs
Please email questions to ACSFscheduling@mdanderson.org. Thank you!
The Advanced Cytometry & Sorting Facility at South Campus (ACSF) is equipped with 5 cell sorters, 6 flow cytometry analysis systems including two Aurora Spectral flow cytometers. The lab provides flow cytometry analysis and cell sorting services.
Leadership
Karen Clise-Dwyer, PhD
ACSF Director
713-563-3322
kcdwyer@mdanderson.org
2SCR4.2020
Core Staff
Name |
Role |
Phone |
Email |
Location |
Karen Clise-Dwyer |
Associate Professor, Director |
713-563-3322 |
kcdwyer@mdanderson.org |
2SCR4.2020 |
Karen Ramirez |
Lab Manager |
713-563-3345 |
kmramirez@mdanderson.org |
2SCR4.2120 |
David Dwyer |
Coordinator, Flow Cytometry Lab |
713-563-3345 |
ddwyer@mdanderson.org |
2SCR4.2120 |
Veena Papanna |
Coordinator, Flow Cytometry Lab |
713-563-3345 |
vpapanna@mdanderson.org |
2SCR4.2120 |
Kathryn Cox |
Coordinator, Flow Cytometry Lab |
713-563-3350 |
kequinta@mdanderson.org |
2SCR4.2213 |
Matthew Ramirez |
Student Extern |
713-563-3345 |
msramirez1@mdanderson.org |
2SCR4.2120 |
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Hours |
Location |
Staffed: 8 AM - 5 PM
Trained User's have access 24/7
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South Campus Research Building 2, Room 2SCR4.2120
7435 Fannin St., Houston, TX 77054
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Location and hours of operation
Links and Resources
- Equipment Scheduling: Email ACSFscheduling@mdanderson.org
- ACSF Core external website https://www.mdanderson.org/research/research-resources/core-facilities/flow-cytometry-and-cellular-imaging-core-facility.html
- Fluorfinder link to ACSF https://app.fluorofinder.com/mdasouth
- Flurofinder OMIPS https://fluorofinder.com/omips/
- FlowJo Recorded Webinars https://www.flowjo.com/learn/webinars/recorded
- FlowJo Tutorial https://www.flowjo.com/learn/flowjo-university/flowjo/getting-started-in-flowjo/131
- FCS Express Recorded Webinars https://denovosoftware.com/videos/
- Flow Cytometry Introduction Video http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html
- Data Analysis Introduction Video http://media.invitrogen.com.edgesuite.net/tutorials/5Data_Analysis/player.html
- Optimized Multicolor Immunofluorescence Panels (OMIPS) https://fluorofinder.com/omips/
- FP Base Spectral Viewer https://www.fpbase.org/spectra/
- BDSelect-Buffer Compatibility Resource https://www.cytobank.org/facselect/
- Antibody Validation Workshop https://go.myabcam.com/antibody-basics-training-email?utm_source=Eloqua&utm_medium=Email&utm_campaign=Welcome|Awareness|2020-02-09%2019:00:00.000|N/A|GLOBAL|EN|Automated%20-%20Lifecycle&utm_content=Email%204|&mi_u=1204290
- BD DIVA Introduction https://static.bdbiosciences.com/training/diva_basics/module1-overview/story_html5.html
- BD DIVA-2 https://static.bdbiosciences.com/training/diva_basics/module2-datadisplay/story_html5.html
- BD DIVA-3 https://static.bdbiosciences.com/training/diva_basics/module4-templates/story_html5.html
- Experimental Design Guide https://myteams.mdanderson.org/depts/sctct/fclabsouth/Shared%20Documents/Experimental%20Design%20Guide.pdf
- ACSF EX002-HN01 V1.1 Antibody Staining Guidelines
- ACSF EX003-HN01 V1.0 Cell Cycle Guidelines
- ACSF EX004-HN01 V1.0 Apoptosis Guidelines
- ACSF TR001-HN01 V1.0 New User Checklist
- ACSF TR001-HN02 V1.1 Cell Sorting Guidelines
- ACSF TR001-HN03 V1.0 Data Analysis Overview
- ACSF TR001-HN04 V1.0 Lab Safety Overview
- Server Login Flow Core Server Login
- Independent Analyzer Training https://myteams.mdanderson.org/depts/sctct/fclabsouth/_layouts/15/WopiFrame.aspx?sourcedoc=/depts/sctct/fclabsouth/Shared%20Documents/ACSF%20TR001-HN07%20V1.1%20Independent%20Analyzer%20Training%20Final.pptx&action=default
- FlowTex: Professional organization that hosts an annual conference and other cytometry education-related events. Most events are free to academic attendees.
- BD Interactive Human Cell Map https://www.bdbiosciences.com/en-us/resources/reagent-selection-tools/interactive-cell-map
- FlowJo_Intro-Refresher+Comp_April 2023
- FlowJo_High Dimensional Data_2023
Online Learning Links
1. Online Learning Links (Select link and open in a new tab)
BD Analyzer Training Videos
- How to replace an empty sheath box
- How to empty waste container
- How to Open DIVA software
- How to Open an Experiment
- How to Open Any Closed Viewing Windows
- How to run a sample
- How to add fluorochrome to the parameter
- How to label marker name for respective fluorochrome
- How to adjust the voltages
- How to create basic plots
- How to draw a gate and label it
- How to do auto compensation 1
- How to do auto compensation 2
- How to clear a partial clog
- What to do if no events show while acquiring sample
- How to clean the instrument after running tube samples
- How to switch from tube mode to plate mode
- How to switch from plate mode to tube mode
- How to run a clean plate
Cytek Aurora Analyzer Training Videos
- How to change the empty sheath and full waste box
- How to startup instrument and run QC
- How to create a new experiment, save a template and open a template
- How to run reference controls into library and reuse in the experiment
- How to clean the instrument after running sample
- How to create batch analysis
- How to export the data and transfer it to OneDrive
- How to shutdown the instrument (general)
- How to shutdown Aurora Rose
- How to shutdown Aurora Blue