Protein Array & Analysis Core (PAAC)

Overview of Services

The Protein Array and Analysis Core (PAAC) at the University of Texas MD Anderson Cancer Center Science Park provides investigators access to innovative and cutting-edge cancer research tools for the discovery and characterization of novel protein-protein interactions. This is accomplished using a state-of-the-art protein microarrayer and additional equipment that will provide biophysical analysis of protein-protein interactions. The PAAC is supported by a grant from Cancer Prevention and Research Institute of Texas (CPRIT).


Apart from identifying novel protein-protein interactions that are mediated between protein peptides and protein domains, we will provide the know-how and instrumentation to determine the strength of these protein-protein interactions. Discovering new nodes of interaction in signal transduction pathways is important because these protein networks are often deregulated in cancers. Importantly, these pathways can be targeted with drugs to inhibit the enzyme responsible for depositing the posttranslational modifications that facilitate many of these interactions. Moreover, protein-protein interactions can be directly targeted with small molecules. These findings will lay the groundwork, and identify the targets, for future cancer drug discovery projects.


Our protein-domain microarray technology facilitates the identification of proteins that interact with motifs that carry phosphorylation, methylation and acetylation modifications. This high-throughput approach allows for the rapid identification of protein-protein interactions in vitro. Additional experiments are then required to confirm that these interactions do indeed occur in vivo and in vitro.


We have developed a chip-size array of proteins that can be used to identify the “readers” of specific PTMs. We are the only research group to have such a large collection of protein domains. Thus, many researchers, who have identified PTMs that are often unique signatures of a certain cancer, send us their modified proteins in the hopes that we can identify relevant binding partners. We are now expanding this facility to not only include state-of-the-art protein domain microarrays, but also to allow the further characterization of these protein-protein interactions in cells. In addition, we will provide the instrumentation and know-how to determine the strength of these protein-protein interactions.


Services Available:

• Probe protein domain microarrays with peptides or small molecules

• Affinity measurements (Kd) for protein complexes

• Stoichiometry of protein complexes by SEC-MALS


Array Types Available:

• pY-Reader - SH2 domain array for phospho-tyrosine ligand analysis.

• pS/T-Reader - BRCT, 14-3-3, & FHA domain array for phospho-threonine and -serine ligand analysis.

• Me-Reader - Tudor and Chromo domain array for methyl-arginine and -lysine ligand analysis.

• Proline-Reader – WW and SH3 domain array for proline-rich ligand analysis.

• Sumo-Reader – An array of Sumo-interacting motifs (SIMs).

• Ac-Reader - Bromo domain array for acetylated lysine ligand analysis.

• Free C-terminal-Readers - PDZ domain array to identify free C-terminal ligands.

• Ub Readers - Ubiquitin-Binding domains array to identify readers of ubiquitin.



For more detailed information, please call or e-mail to set up a meeting with Core staff. Contact Mark Bedford ( for experiments involving the use of protein domain microarrays, and contact Shawn Bratton ( for experiments involving the biophysical analysis of protein complexes.


PAAC User Group Membership Request

The members of the PAAC User Group need to be Texas-based cancer researchers.


Members of the PAAC User Group benefit from a reduced fee structure and a yearly allotment of $3,000 for use in the Core. In order to receive this support, you will have to complete a “Service Request Form” and have the Project approved for funding. Applications will be vetted first by the PAAC Directors and then by the PAAC Oversight Committee.


There is the possibility that new members can be added to the current PAAC user group.


To become a new member of the PAAC user group you will need to submit a “Membership Application Form” to Mark Bedford ( Applications will be vetted by the PAAC Directors and then by the PAAC Oversight Committee (Drs. Dent, Johnson, Wood and Dalby).




Mark T. Bedford, Ph.D - Director



Shawn B. Bratton, Ph.D - Co-Director




UT MD Anderson Cancer Center    

1808 Park Road 1C

Smithville, TX  78957-0389

Links and Resources

  1. Protein Array & Analysis Core
  2. Dr. Mark T. Bedford Laboratory
  3. Dr. Shawn B. Bratton Laboratory


Name Role Phone Email Location
Cari Sagum
Protein Domain Microarray Staff

Members at UT System

Core Name Primary Contact Email Phone Number/Ext
Bionutrition Research Core

Christine Ranieri, MS, RD, LD 713-794-1295
Biospecimen Extraction Facility

<p>The Biospecimen Extraction Resource BER provides a centralized laboratory for standardized, high quality DNA, RNA and protein extraction from blood, tissues, saliva, cells, or other patientderived biospecimens.</p>

Jian Gu, Ph.D. 713-792-8016
Bone Histomorphometry Core Laboratory

The Bone Histomorphometry Core Laboratory provides histomorphometric analyses of nondecalcified bone specimens, including processing mouse bone specimens for nondecalcified histology and performing static and dynamic histomorphometric analyses of mouse bone specimens.

Michael Starbuck 713-563-1212
Characterized Cell Line Core Facility

The Characterized Cell Line distributes well characterized and validated cell lines to researchers across the world and offers cell line validation using short tandem repeats STR profiling.

Keri Sherman 713-792-5743
Chemical Imaging Research Core

Dodge Baluya 713-794-1661
Core for Biomolecular Structure and Function (CBSF)

<p>The purpose of the Core for Biomolecular Structure and Function (CBSF) is to provide recombinant&nbsp;protein production, protein characterization, binding studies and structural biology (X-ray crystallography)&nbsp;services to MD Anderson Cancer Center and the wider Texas Medical Center.</p>

Paul Leonard 832-750-1516
DNA Methylation Core

The DNA Methylation Core is funded by the Center for Cancer Epigenetics and provides services related to the analysis of DNA methylation for researchers at the University of Texas M.D. Anderson Cancer Center.

Marcos Estecio 713-792-9108
Gene Editing/Cellular Model Core Facility

The Gene Editing Cellular Model Core Facility provides custom cellular model construction services using two technical platforms.

Dr. Junjie Chen 713-792-4863
Genetically Engineered Mouse Facility (GEMF)

<p>The Genetically Engineered Mouse Facility provides unique models with wide application to basic and translational research. The GEMF supplies indispensable expertise to generate these models in an efficient and costeffective manner using stateoftheart procedures.</p>

Jan Parker-Thornburg, Ph.D. 713-745-2654, 713-745-1419 (lab)
High Resolution Electron Microscopy Facility (HREMF)

The mission of the High Resolution Microscopy Facility is to provide scanning and transmission electron microscopy services to researchers.

Robert R. Langley, Ph.D. 713-792-9142, 713-792-8747 (fax)
Laboratory Animal Genetic Services

The Laboratory Animal Genetic Service Core provides services for speed congenics, background characterization, microbial PCR testing, rodent STR and murine genetic consultations.

Fernando Benavides, DVM, PhD, DACLAM 512-237-9343
Molecular Cytogenetics Core Facility

The primary goal of this facility is to offer conventional and molecular cytogenetic services, including species identification, karyotyping, analysis of genomic instability, fluorescence in situ hybridization and spectral karyotyping

Asha S. Multani Ph.D. 713-563-1892
Monoclonal Antibody Core Facility

The Monoclonal Antibody Facility develops high quality, cost effective customized MAbs to meet the basic, translational, and clinical research needs of MD Anderson investigators.

Laura Bover 713-563-3301 or 713-563-2281
NORTH Campus Flow Cytometry and Cellular Imaging Core Facility

The Flow Cytometry and Cellular Imaging Facility was established to provide access to state of the art cell analysis technology for researchers, and provides cell sorting, analytical flow cytometry, cellular imaging and custom monoclonal antibody mAb conjugations to its users.

Joan Hoover-Zuniga 713-745-1749
Nuclear Magnetic Resonance Facility

The NMR facility is used for structure determination of compounds developed in drug discovery, pharmacology, and diagnostic imaging laboratories.

John S. McMurray, Ph.D 713-745-3763

PROSPR provides assistance to researchers with collection and assessment of Patient Reported Outcomes, behavioral data, and measures of fitness and body composition.

Susan K. Peterson, PhD, MPH 713-792-8267
Pharmaceutical Chemistry Facility

The mission of the Pharmaceutical Chemistry Facility is to assist researchers with the design and synthesis of compounds for use in cancer research.

Barbara Czako Ph.D 713-745-2050
Protein Array & Analysis Core (PAAC)

The Protein Array and Analysis Core PAAC at the University of Texas MD Anderson Cancer Center Science Park provides investigators access to innovative and cutting edge cancer research tools for the discovery and characterization of novel proteinprotein interactions.

Cari Sagum 512-237-9328
Proteomics and Metabolomics

<p>The mission of the Proteomics and Metabolomics Core Facility is to provide the research community with cutting edge expertise and services for sensitive, precise, and accurate measurement of proteins and metabolites using mass spectrometrybased technologies.p</p>

David Hawke, Ph.D 713-834-6096
RPPA-Functional Proteomics

Functional Proteomics Reverse Phase Protein Array RPPA Core provides cancer center members with access to a powerful, highthroughput, quantitative costeffective antibodybased assay to characterize basal or ligandinduced protein expression and modification, and timeresolved responses appropriat

Keri Sherman 713-792-5743
Research Histology Core Laboratory

The Research Histology Core Laboratory includes specialized equipment for immunohistochemistry, fluorescent in situ hybridization FISH, laser capture microdissection, and tissue microarray construction.

Jacqueline Furr 713-792-3119, 713-563-0544 (fax)
Science Park Flow Cytometry and Cell Imaging Core

The Flow Cytometry and Cell Imaging Core Facility at Science Park provides cutting edge cell analysis resources. These include cell sorting and analytical flow cytometry to phenotype, quantify and isolate cells and confocal microscopy to capture high resolution images.p

Pamela Whitney - Lab 512-237-9427
Science Park Molecular Biology Core

The Molecular Biology Core Facility at Science Park provides investigators access to sophisticated and stateoftheart molecular techniques, services and instrumentation.

Jianjun Shen, Ph.D.
Science Park NGS

The Science Park NGS facility is built around a user group of 30 cancer researchers at MD Anderson Science Park, UT Austin, and Texas State University San Marcos.

Jianjun Shen, Ph.D
Science Park Research Histology, Pathology & Imaging Core

The Science Park Research Histology, Pathology Imaging Core provides investigators with expert imaging and experimental pathology support. Our staff consult with investigators to formulate an integrated imaging, pathology and histology protocol for each study.

Manu Sebastian, DVM, PhD, DACVP, DABT, DECLAM 512-237-9347
Sequencing and Microarray Facility (SMF)

The Sequencing and Microarray Facility provides essential genomic analysis services to support researchers in basic science, translational, and populationbased research programs

Erika Thompson, M.S. 713-834-6381
Sequencing and ncRNA Program

The Sequencing and Noncoding RNA Program is one of an MDA institution supported shared genomic service resources providing not only instrumentation but also expertise in applications of next generation sequencing and microarray services to investigators

Chang-gong Liu, Ph.D. 713-792-2287
Small Animal Imaging Facility

The Small Animal Imaging Facility provides access to imaging instrumentation and expertise that allows researchers to integrate powerful and cuttingedge imaging technologies into routine preclinical research with confidence that their scientific objectives will be achieved.

Charles Kingsley (713) 563-6709
shRNA and ORFeome Core

The shRNA and ORFeome core Facility makes the following libraries available to MD Anderson members human and murine shRNA libraries, human ORFeome collaboration and LentiORF libraries. The core not only provides center members with individual shRNA and ORF clones but also builds customized librar...

Dr. Sun, Yutong (713) 792-3677
siRNA Core Facility

The siRNA Core Facility at MD Anderson performs High Throughput and High Content siRNA screens to assist experimental cancer therapy studies. The cores ultimate goal is to develop relevant three dimensional models that represent the human body..

The University of Texas at Arlington

Core Name Primary Contact Email Phone Number/Ext
The Shimadzu Center for Advanced Analytical Chemistry Maciej Kukula
The Shimadzu Center for Bio-Molecular Imaging Jeff Campbell
The Shimadzu Institute NanoTechnology Research Center Nader Hozhabri
Core Name Primary Contact Email Phone Number/Ext
Kleberg Advanced Microscopy Center

KAMC focuses on the synthesis and characterization of nanomaterials for imaging, labels for bioassays, and active targeting for in vivo or in vitro diagnostics. The Core studies the interaction of nanoparticles with living cells for application in the targeted delivery of drugs, genes, and proteins.

Miguel Jose-Yacaman, Ph.D. (210) 458-6954
Protein Biomarker Core

The RCMI Proteomics and Protein Biomarkers Core Facility is focused on capillary liquid chromatographymass spectrometry LCMS and tandem mass spectrometry LCMSMS to identify, characterize, and quantify proteins. The Proteomics Core develops novel methods, while the Protein Biomarkers Core applies ...

Stephan Bach, Ph.D. (210) 458-4466
RCMI Biophotonics Core

The RCMI Biophotonics Core Facility provides cutting edge technology for the study and manipulation of biological samples using light, including longterm indepth imaging of live tissues and the technology to probe at the molecular level for proteinprotein interactions within live samples.

George Perry, Ph.D. (210) 458-4450

University of Texas Health Northeast

Core Name Primary Contact Email Phone Number/Ext
Core Name Primary Contact Email Phone Number/Ext
Atomic Force Microscopy

Ana Maria Zaske, PhD 713-486-5418
Biomedical Informatics Group (BIG)

We offer services to researchers, including access to clinical data and enhanced subject recruiting, and are involved in multiple local and national collaborations with other groups.

Susan Guerrero 713-500-3926
CCTS Biobank

The CCTS Biobank consists of a collection of human samples and related clinical and genomic data that are available for collaborative sharing with qualified researchers and owned by contributing investigators conducting research within the Texas Medical Center.

Mary Hall, MBA, PhD 713.500.2092
Cellular Therapy Core

<p>The CTC is an FDA registered facility where tissues are processed to produce cells for clinical applications. It is focused on translating, validating and supporting IND applications at a preclinical level into clinical grade processes.</p>

Fabio Triolo, Ph.D. 713-486-2542
Center for Advanced Microscopy

The Center for Advanced Microscopy provides assisted or unassisted state of the art imaging capabilities including three confocal microscopes, fluorescence lifetime imaging, live animal imaging with an IVIS Lumina, flow cytometry, and a liquid handling multiwell plate reader.

Olga Chumakova, Ph.D. 713-500-7495
Flow Cytometry Service Center

The Flow Cytometry Service Center provides a broad range of assisted and unassisted flow cytometry services from basic 2 color analysis to advanced 14 parameter analysis and multi population cell sorting.

Amy Hazen, Ph.D. 713-500-2486
Human Genetics Center Laboratory

Megan Grove, MS 713-500-9800
Magnetic Resonance Imaging Core

The MR group provides advanced MRI techniques, including selective tissue imaging, magnetic resonance spectroscopic imaging, diffusion tensor imaging to neurological disorders, central nervous system trauma, and drug addiction both in humans and animal models.

Ponnada Narayana, Ph.D 713-500-7677
Nano Chemistry Service Center

The Nanochemistry Service Center provides provides custom synthesis, labeling, and chemical coupling of DNA thioaptamers and nanoparticles and large-scale, high-resolution 3D printing services.

David Volk, PhD 713-500-3611
Proteomics Service Center

The CTPC services provided are designed to identify and quantitate proteins and their modifications in a broad range of research specimens from simple purified protein samples to biomarker discovery and verification in complex mixtures such as cell and tissue extracts, plasma andor other biofluids

Li Li 713-500-2456
Quantitative Genomics & Microarray Service Center

The Quantitative Genomics Microarray Core Lab can perform all techniques associated with a qPCR instrument, quantitative Meso Scale ELISAs and microarrays utilizing Illumina and Agilent arrays.

David Loose, Ph.D. 713-500-7440
Therapeutic Antibody Core

The Therapeutic Antibody Core aims to provide statewide support and service to advance lead antibodies from academic laboratories to the stage of preclinical development.

Zhiqiang An, PhD 713-500-3011
Transgenic and Stem Cells Service Center

Eva Zsigmond, PhD 713-500-2453
Core Name Primary Contact Email Phone Number/Ext
Advanced Nucleic Acids Core Facility

Brian Wickes (210) 567-3938
Bioanalytics and Single-Cell Core

Nameer Kirma (210) 562-4068
Biobanking and Genome Analysis

Teresa L. Johnson-Pais, Ph.D. 210.567.6571
Center for Macromolecular Interactions

Flow Cytometry Core Facility

Benjamin Daniel 210-567-3911
Mass Spectrometry Laboratory

Susan Weintraub, Ph.D. (210) 567-4043
MicroCT Core Laboratory

Roberto Fajardo 210-567-6176
NMR / Screening

Kristin E. Cano, Ph.D. 210-567-8706
Optical Imaging Facility

Exing Wang 210-562-4062
X-Ray Crystallography

John Hart 210-567-0751

University of Texas Medical Branch - Galveston

Core Name Primary Contact Email Phone Number/Ext
Biomolecular Resource Facility

William Russell, Ph.D (409) 772-3579
International Biosafety Training Center

Sharon Walters 409-772-1781
Research Histopathology Core

The Research Histopathology Core is equipped with the latest available instrumentation. RHC’s instrumentation is operated by highly skilled personnel available to assist in tissue preparation, immunohistochemistry, immunofluorescence, Insitu hybridization, including assay optimization, image anal...

Gustavo Valbuena (409) 747-0735
Core Name Primary Contact Email Phone Number/Ext
Clinical Research Unit (CRU)/Investigational Drug Service (IDS) Lisa Fleming, MPA 214.648.2718
Flow Cytometry Facility at UTSW David Farrar 24-648-7315
Genomics Sequencing & Microarray Core Facility Chaoying Liang 214-648- 7329
High Throughput Screening Core Bruce Posner, Ph.D. 214-648-0285
Metabolic Phenotyping Core Ruth Gordillo 214-648-3403
O'Brien Kidney Center: Animal Models Core Lauren Diana Smith 214-648-7222
O'Brien Kidney Center: Cell Biology and Imaging Core Lauren Diana Smith 214-648-7222
O'Brien Kidney Center: Clinical and Translational Research Core Lauren Diana Smith 214-648-7222
O'Brien Kidney Center: Physiology Core Lauren Diana Smith 214-648-7222
Preclinical Pharmacology Core Noelle Williams 214-648-5072
Rodent Behavior Core Shari Birnbaum 214-648-5123
Structural Biology Laboratory
Whole Brain Microscopy Facility (WBMF) Denise Ramirez, PhD 214-648-0203