MDACC EMC Flow Cytometry and Cell Imaging Core

Overview of Services

The Flow Cytometry and Cell Imaging Core Facility in the Department of Epigenetics and Molecular Carcinogenesis at South Campus provides cutting-edge resources to dissect the cellular and molecular mechanisms of developmental and disease processes. Flow cytometry allows researchers to quantify and isolate rare viable cell subsets from heterogenous populations in single-cell suspensions based on differential expression of surface antigens. Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric analysis, sorting of fluorescence protein-tagged cells, assessing apoptosis and cell cycle status, the identification/isolation of stem and cancer stem cells (e.g., marker positive, side-population, etc.) and the deposition of single cells. Multispectral confocal imaging permits high resolution imaging of cells and expression/localization of molecules of interest in a physiologically relevant context in situ. Additional confocal applications include microirradiation to study DNA-damage repair, protein-protein interaction assays (e.g., FRET), 3D reconstructions and time-lapse imaging of dynamic cell and molecular processes. The Leica TCS SP8 DIVE multiphoton (MP) microscope uses infrared excitation light, which provides for deeper penetration into thicker tissues and can be used to examine thick sections, intact organs, and in vivo processes with maximal imaging depth, minimal light scattering, increased z-resolution, and reduced phototoxicity. Z-stacks combined with tile scans can be volumetrically rendered to generate 3D reconstructions and for morphometric analysis. The INCell permits rapid multispectral, wide-field imaging of multiwell plates and slides. Wetlab services include immunofluorescence staining, vibratome sectioning, tissue clearing and mounting and a fluorescence dissection scope is provided for tissue harvesting and documentation. The FCCIC also offers individualized training on all instruments and assistance with data analysis and experimental design.

Leadership

Co-Directors:

Dr. Ellen Richie

erichie@mdanderson.org

832-750-7181

Dr. Collene Jeter

cjeter@mdanderson.org

832-750-7259

Location and Hours of Operation

The UT MD Anderson Cancer Center South Campus, 3SCRB (Cell Imaging) and 4SCRB (Flow Cytometry)

Hours

Location

Monday - Friday

9 am - 5 pm

1901 East Road

Houston, TX 77054

Links and Resources

Flow Cytometry Core

Flow Cytometry Core Internal

Cell Imaging

Contacts

Name	Role	Phone	Email	Location
Pamela Whitney - Lab	Flow Cytometry Manager	832-750-7201	pjwhitney@mdanderson.org	4SCRB4.2026 & 2028
Pamela Whitney - Office	Flow Cytometry Manager	832-750-7179		4SCRB4.1347
Collene Jeter - Lab	Cell Imaging Manager		cjeter@mdanderson.org	3SCRB2.3210
Collene Jeter - Office	Cell Imaging Manager	832-750-7259		3SCRB4.3420

Service list

Name	Description	Price
AutoMACS Cell Separation		Inquire
Axio Observer (Epifluorescence) Assisted	Hourly rate	Inquire
Axio Observer (Epifluorescence) Independent	Hourly rate; <30 min = no charge	Inquire
Axio Observer Time-Lapse (>8 hours)	Hourly rate (12 h max rate per day) Set up fee may be required Burner fee required for fluorescence imaging	Inquire
Axio Observer Training	30 min; further assistance charged at Assisted rate	Inquire
BD FACSAria Fusion - Analysis Assisted	Hourly rate	Inquire
BD FACSAria Fusion - Analysis Independent	Hourly rate	Inquire
BD FACSAria Fusion - Set Up Fee	Hourly rate Instrument set up fee for unassisted analysis/sort as needed Applicable to late, no show and failed experiments (cancellations within 1 business day of appointment)	Inquire
BD FACSAria Fusion - Sort Assisted	Hourly rate	Inquire
BD FACSAria Fusion - Sort Independent	Hourly rate	Inquire
BD FACSAria SORP - Analysis Assisted	Hourly rate	Inquire
BD FACSAria SORP - Analysis Independent	Hourly rate	Inquire
BD FACSAria SORP - Set Up Fee	Hourly rate Instrument set up fee for unassisted analysis/sort as needed Applicable to late, no show and failed experiments (cancellations within 1 business day of appointment)	Inquire
BD FACSAria SORP - Sort Assisted	Hourly rate	Inquire
BD FACSAria SORP - Sort Independent	Hourly rate	Inquire
BD LSRFortessa - Analysis Assisted	Hourly rate	Inquire
BD LSRFortessa - Analysis Independent	Hourly rate	Inquire
Flow Cytometry Consultation		Inquire
Flow Jo Data Analysis	Per experiment	Inquire
Imaging Consultation	No charge	Inquire
Imaris Image Processing Assisted	Hourly rate	Inquire
Imaris Image Processing Independent	Hourly rate	Inquire
Imaris Image Processing Training	Hourly rate	Inquire
INCell Assisted	Hourly rate	Inquire
INCell Training	30 min; further assistance charge at Assisted rate	Inquire
INCell Unassisted	Slide/Plate scanning	Internal $17.88 each External in network $17.88 each External out of network $28.97 each UT-System $17.88 each Texas Institutions $18.83 each
Introductory Training / Clean-up or Unclogging Fee	As necessary, additional one time fee for first time users, post-reservation administrative clean-up, or instrumentation unclogging.	Inquire
LifeCanvas Active Clearing	Detergent-based and electrophoretically enhanced whole organ clearing Consultation with core staff prior to sample preservation/submission required *SHIELD preservation REQUIRED May not be compatible with all tissue/organ types and/or epitopes	Inquire
LifeCanvas Active IF Staining	Electrophoretically enhanced whole organ IF staining Compatible with LifeCanvas validated antibodies; additional may be tested (see core staff) *Antibodies not provided as part of service fee Consultation with core staff prior to sample preservation/submission required May not be compatible with all tissue/organ types and/or epitopes	Inquire
LifeCanvas Passive Tissue Clearing	Detergent-based tissue clearing Consultation with core staff prior to sample preservation/submission required *SHIELD preservation recommended May not be compatible with all tissue/organ types and/or epitopes	Inquire
LSM880 (Confocal) Assisted	Hourly rate	Inquire
LSM880 (Confocal) Independent	Hourly rate	Inquire
LSM880 (Confocal) Time-Lapse	Hourly rate (12 h max rate per day) Available weekends (3 day maximum) Set up fee may be required	Inquire
LSM880 (Confocal) Training	Hourly rate	Inquire
Primary Antibody/Dye	Per sample/coverslip	Inquire
Standard Immunofluorescence Staining	Per slide Core provided primary antibody/dye charged separately Additional fee for FFPE processing	Inquire
Standard New Antibody Development	6 sample maximum Confocal imaging charged separately as needed	Inquire
TCS SP8 (Multiphoton) Assisted	Hourly rate	Inquire
TCS SP8 (Multiphoton) Training	With core management approval 4 hr miminum training; further assistance charged at Assisted rate	Inquire
TCS SP8 (Multiphoton) Unassisted	Requires prior TCS SP8 training and core management approval Can be added to assisted rate following sample set-up for long sample runs	Inquire
Thick Tissue New Antibody Development	6 sample maximum Multiphoton imaging charged separately	Inquire
Thick Tissue Staining	Core provided primary antibody/dye charged separately	Inquire
Vibratome Sectioning Assisted	Per sample 10 sections per sample maximum	Inquire
Vibratome Sectioning Training	Per hour	Inquire
Vibratome Sectioning Unassisted	Per sample; 10 sections per sample maximum Subject to availability/approval	Inquire

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