Welcome!
ALERT The Flow Cytometry and Cell Imaging Core Facility in the Department of Epigenetics and Molecular Carcinogenesis at South Campus provides cutting-edge resources to dissect the cellular and molecular mechanisms of developmental and disease processes. Flow cytometry allows researchers to quantify and isolate rare viable cell subsets from heterogenous populations in single-cell suspensions based on differential expression of surface antigens. Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric analysis, sorting of fluorescence protein-tagged cells, assessing apoptosis and cell cycle status, the identification/isolation of stem and cancer stem cells (e.g., marker positive, side-population, etc.) and the deposition of single cells. Multispectral confocal imaging permits high resolution imaging of cells and expression/localization of molecules of interest in a physiologically relevant context in situ. Additional confocal applications include microirradiation to study DNA-damage repair, protein-protein interaction assays (e.g., FRET), 3D reconstructions and time-lapse imaging of dynamic cell and molecular processes. The Leica TCS SP8 DIVE multiphoton (MP) microscope uses infrared excitation light, which provides for deeper penetration into thicker tissues and can be used to examine thick sections, intact organs, and in vivo processes with maximal imaging depth, minimal light scattering, increased z-resolution, and reduced phototoxicity. Z-stacks combined with tile scans can be volumetrically rendered to generate 3D reconstructions and for morphometric analysis. The INCell permits rapid multispectral, wide-field imaging of multiwell plates and slides. Wetlab services include immunofluorescence staining, vibratome sectioning, tissue clearing and mounting and a fluorescence dissection scope is provided for tissue harvesting and documentation. The FCCIC also offers individualized training on all instruments and assistance with data analysis and experimental design.
Co-Directors:
Dr. Ellen Richie
erichie@mdanderson.org
832-750-7181
Dr. Collene Jeter
cjeter@mdanderson.org
832-750-7259
The UT MD Anderson Cancer Center South Campus, 3SCRB (Cell Imaging) and 4SCRB (Flow Cytometry)
| Hours | Location |
|
Monday - Friday 9 am - 5 pm |
1901 East Road Houston, TX 77054 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Pamela Whitney - Lab |
Flow Cytometry Manager
|
832-750-7201
|
pjwhitney@mdanderson.org
|
4SCRB4.2026 & 2028
|
| Pamela Whitney - Office |
Flow Cytometry Manager
|
832-750-7179
|
4SCRB4.1347
|
|
| Collene Jeter - Lab |
Cell Imaging Manager
|
cjeter@mdanderson.org
|
3SCRB2.3210
|
|
| Collene Jeter - Office |
Cell Imaging Manager
|
832-750-7259
|
3SCRB4.3420
|
| Service list |
| ► Confocal & Cell Imaging (29) | |||
| Name | Description | Price | |
|---|---|---|---|
| Axio Observer (Epifluorescence) Assisted |
Hourly rate |
Inquire | |
| Axio Observer (Epifluorescence) Independent |
Hourly rate; <30 min = no charge |
Inquire | |
| Axio Observer Time-Lapse (>8 hours) |
Hourly rate (12 h max rate per day) |
Inquire | |
| Axio Observer Training |
30 min; further assistance charged at Assisted rate |
Inquire | |
| Imaging Consultation |
No charge |
Inquire | |
| Imaris Image Processing Assisted |
Hourly rate |
Inquire | |
| Imaris Image Processing Independent |
Hourly rate |
Inquire | |
| Imaris Image Processing Training |
Hourly rate |
Inquire | |
| INCell Assisted |
Hourly rate |
Inquire | |
| INCell Training |
30 min; further assistance charge at Assisted rate |
Inquire | |
| INCell Unassisted |
Slide/Plate scanning |
Internal
$17.88
each
External in network $17.88 each External out of network $28.97 each UT-System $17.88 each Texas Institutions $18.83 each |
|
| LifeCanvas Active Clearing |
Detergent-based and electrophoretically enhanced whole organ clearing Consultation with core staff prior to sample preservation/submission required *SHIELD preservation REQUIRED May not be compatible with all tissue/organ types and/or epitopes |
Inquire | |
| LifeCanvas Active IF Staining |
Electrophoretically enhanced whole organ IF staining Compatible with LifeCanvas validated antibodies; additional may be tested (see core staff) *Antibodies not provided as part of service fee Consultation with core staff prior to sample preservation/submission required May not be compatible with all tissue/organ types and/or epitopes |
Inquire | |
| LifeCanvas Passive Tissue Clearing |
Detergent-based tissue clearing Consultation with core staff prior to sample preservation/submission required *SHIELD preservation recommended May not be compatible with all tissue/organ types and/or epitopes |
Inquire | |
| LSM880 (Confocal) Assisted |
Hourly rate |
Inquire | |
| LSM880 (Confocal) Independent |
Hourly rate |
Inquire | |
| LSM880 (Confocal) Time-Lapse |
Available weekends (3 day maximum) Set up fee may be required
|
Inquire | |
| LSM880 (Confocal) Training |
Hourly rate |
Inquire | |
| Primary Antibody/Dye |
Per sample/coverslip |
Inquire | |
| Standard Immunofluorescence Staining |
Per slide Core provided primary antibody/dye charged separately Additional fee for FFPE processing |
Inquire | |
| Standard New Antibody Development |
6 sample maximum Confocal imaging charged separately as needed
|
Inquire | |
| TCS SP8 (Multiphoton) Assisted |
Hourly rate |
Inquire | |
| TCS SP8 (Multiphoton) Training |
With core management approval 4 hr miminum training; further assistance charged at Assisted rate |
Inquire | |
| TCS SP8 (Multiphoton) Unassisted |
Requires prior TCS SP8 training and core management approval Can be added to assisted rate following sample set-up for long sample runs |
Inquire | |
| Thick Tissue New Antibody Development |
6 sample maximum Multiphoton imaging charged separately |
Inquire | |
| Thick Tissue Staining |
Core provided primary antibody/dye charged separately |
Inquire | |
| Vibratome Sectioning Assisted |
Per sample 10 sections per sample maximum |
Inquire | |
| Vibratome Sectioning Training |
Per hour
|
Inquire | |
| Vibratome Sectioning Unassisted |
Per sample; 10 sections per sample maximum Subject to availability/approval |
Inquire | |
| ► Flow Cytometry (16) | |||
| Name | Description | Price | |
| AutoMACS Cell Separation | Inquire | ||
| BD FACSAria Fusion - Analysis Assisted |
Hourly rate |
Inquire | |
| BD FACSAria Fusion - Analysis Independent |
Hourly rate |
Inquire | |
| BD FACSAria Fusion - Set Up Fee |
Hourly rate Instrument set up fee for unassisted analysis/sort as needed Applicable to late, no show and failed experiments (cancellations within 1 business day of appointment) |
Inquire | |
| BD FACSAria Fusion - Sort Assisted |
Hourly rate |
Inquire | |
| BD FACSAria Fusion - Sort Independent |
Hourly rate |
Inquire | |
| BD FACSAria SORP - Analysis Assisted |
Hourly rate |
Inquire | |
| BD FACSAria SORP - Analysis Independent |
Hourly rate |
Inquire | |
| BD FACSAria SORP - Set Up Fee |
Hourly rate Instrument set up fee for unassisted analysis/sort as needed Applicable to late, no show and failed experiments (cancellations within 1 business day of appointment) |
Inquire | |
| BD FACSAria SORP - Sort Assisted |
Hourly rate |
Inquire | |
| BD FACSAria SORP - Sort Independent |
Hourly rate |
Inquire | |
| BD LSRFortessa - Analysis Assisted |
Hourly rate |
Inquire | |
| BD LSRFortessa - Analysis Independent |
Hourly rate |
Inquire | |
| Flow Cytometry Consultation | Inquire | ||
| Flow Jo Data Analysis |
Per experiment |
Inquire | |
| Introductory Training / Clean-up or Unclogging Fee |
As necessary, additional one time fee for first time users, post-reservation administrative clean-up, or instrumentation unclogging. |
Inquire | |
