The Flow Cytometry and Cell Imaging Core Facility in the Department of Epigenetics and Molecular Carcinogenesis at South Campus provides cutting-edge resources to dissect the cellular and molecular mechanisms of developmental and disease processes. Flow cytometry allows researchers to quantify and isolate rare viable cell subsets from heterogenous populations in single-cell suspensions based on differential expression of surface antigens. Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric analysis, sorting of fluorescence protein-tagged cells, assessing apoptosis and cell cycle status, the identification/isolation of stem and cancer stem cells (e.g., marker positive, side-population, etc.) and the deposition of single cells. Multispectral confocal imaging permits high resolution imaging of cells and expression/localization of molecules of interest in a physiologically relevant context in situ. Additional confocal applications include microirradiation to study DNA-damage repair, protein-protein interaction assays (e.g., FRET), 3D reconstructions and time-lapse imaging of dynamic cell and molecular processes. The Leica TCS SP8 DIVE multiphoton (MP) microscope uses infrared excitation light, which provides for deeper penetration into thicker tissues and can be used to examine thick sections, intact organs, and in vivo processes with maximal imaging depth, minimal light scattering, increased z-resolution, and reduced phototoxicity. Z-stacks combined with tile scans can be volumetrically rendered to generate 3D reconstructions and for morphometric analysis. The INCell permits rapid multispectral, wide-field imaging of multiwell plates and slides. Wetlab services include immunofluorescence staining, vibratome sectioning, tissue clearing and mounting and a fluorescence dissection scope is provided for tissue harvesting and documentation. The FCCIC also offers individualized training on all instruments and assistance with data analysis and experimental design.
Co-Directors:
Dr. Ellen Richie
erichie@mdanderson.org
832-750-7181
Dr. Collene Jeter
cjeter@mdanderson.org
832-750-7259
The UT MD Anderson Cancer Center South Campus, 3SCRB (Cell Imaging) and 4SCRB (Flow Cytometry)
Hours | Location |
Monday - Friday 9 am - 5 pm |
1941 East Road Houston, TX 77054 |
Name | Role | Phone | Location | |
---|---|---|---|---|
Pamela Whitney - Lab |
Flow Cytometry Manager
|
832-750-7201
|
pjwhitney@mdanderson.org
|
4SCRB4.2026 & 2028
|
Pamela Whitney - Office |
Flow Cytometry Manager
|
832-750-7179
|
4SCRB4.1347
|
|
Collene Jeter - Lab |
Cell Imaging Manager
|
cjeter@mdanderson.org
|
3SCRB2.3210
|
|
Collene Jeter - Office |
Cell Imaging Manager
|
832-750-7259
|
3SCRB4.3420
|
Service list |
► Confocal & Cell Imaging (23) | |||
Name | Description | Price | |
---|---|---|---|
Axio Observer (Epifluorescence) Assisted |
Hourly rate |
Inquire | |
Axio Observer (Epifluorescence) Independent |
Hourly rate |
Inquire | |
Axio Observer Time-Lapse (>8 hours) |
Hourly rate (12 h max rate per day) |
Inquire | |
Burner Fee | Inquire | ||
Imaging Consultation |
No charge |
Inquire | |
Imaging Set Up Fee |
Hourly rate, charged in 30 min increments Instrument set up fee for time lapse as needed Applicable to no show and failed experiments (cancellations within 1 business day of appointment) |
Inquire | |
Imaris Image Processing Assisted |
Hourly rate |
Inquire | |
Imaris Image Processing Independent |
Hourly rate |
Inquire | |
Imaris Image Processing Training |
Hourly rate |
Inquire | |
INCell Assisted |
Hourly rate |
Inquire | |
LSM880 (Confocal) Assisted |
Hourly rate |
Inquire | |
LSM880 (Confocal) Independent |
Hourly rate |
Inquire | |
LSM880 (Confocal) Time-Lapse |
Hourly rate (12 h max rate per day) Available weekends (3 day maximum) Set up fee may be required
|
Inquire | |
LSM880 (Confocal) Training |
Hourly rate |
Inquire | |
Primary Antibody/Dye |
Per sample/coverslip |
Inquire | |
Standard Immunofluorescence Staining |
Per slide Core provided primary antibody/dye charged separately Additional fee for FFPE processing |
Inquire | |
Standard New Antibody Development |
10 sample maximum Confocal imaging charged separately as needed FFPE processing fee charged separately as needed
|
Inquire | |
TCS SP8 (Multiphoton) Assisted |
Hourly rate |
Inquire | |
Thick Tissue New Antibody Development |
5 sample maximum Multiphoton imaging charged separately |
Inquire | |
Thick Tissue Staining |
Core provided primary antibody/dye charged separately |
Inquire | |
Vibratome Sectioning Assisted |
Per sample 10 sections per sample maximum |
Inquire | |
Vibratome Sectioning Training |
Per hour
|
Inquire | |
Vibratome Sectioning Unassisted |
Per hour |
Inquire | |
► Flow Cytometry (15) | |||
Name | Description | Price | |
Aria II Cell Sort Assisted |
Hourly rate |
Inquire | |
Aria II Cell Sort Independent |
Hourly rate |
Inquire | |
Aria II Immunofluorescence Analysis Assisted |
Hourly rate |
Inquire | |
Aria II Immunofluorescence Analysis Independent |
Hourly rate |
Inquire | |
AutoMACS Cell Separation | Inquire | ||
Flow Cytometry Consultation | Inquire | ||
Flow Cytometry Set Up Fee |
Hourly rate Instrument set up fee for unassisted analysis/sort as needed Applicable to late, no show and failed experiments (cancellations within 1 business day of appointment) |
Inquire | |
Flow Jo Data Analysis |
Per experiment |
Inquire | |
Fortessa Immunofluorescence Analysis Assisted |
Hourly rate |
Inquire | |
Fortessa Immunofluorescence Analysis Independent |
Hourly rate |
Inquire | |
Fusion Cell Sort Assisted |
Hourly rate |
Inquire | |
Fusion Cell Sort Independent |
Hourly rate |
Inquire | |
Fusion Immunofluorescence Analysis Assisted |
Hourly rate |
Inquire | |
Fusion Immunofluorescence Analysis Independent |
Hourly rate |
Inquire | |
Introductory Training |
Flat fee |
Inquire |