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Users are required to complete an ORION iLAB service request at least 24 hours prior to sample submission for all Luminex, MSD and batch flow cytometry requests. Sample boxes must be labeled with an iLab service ID.Please include the following acknowledgement in research publications supported by the core: "Supported by the NIH/NCI under award number P30 CA016672 and used the ORION Core". |
The purpose of our core is to provide state-of-the-art immunoprofiling services to MDACC investigators as well as local investigators. Our services range from single service instrument use to entire project design and analysis. Researchers and Investigators will need to provide the samples for analysis. Samples can be stored short-term prior to assessment.
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Services:
Equipment and Technology:
Cara Haymaker, Ph.D. | Director | (713) 792-1458 | chaymaker@mdanderson.org
Location | Hours |
2SCR2.3219, 7435 Fannin Street Houston, TX 77054 |
Monday-Friday 10:00am-6:00pm |
ORION Website
MDACC Courier Services:
Phone: 713-792-7491
Email: Courier@mdanderson.org
Please ship all samples to: 2SCR2.3219
Luminex
Kit Vendors:
Sales representative: Cody Brunetta | cody.brunetta@thermofisher.com
Sales representative: Christof Straub | christof_straub@bio-rad.com
Sales representative: Fabiana Morales | fabiana.morales@milliporesigma.com
Sales representative: Tracy Everingham | tracy.everingham@bio-techne.com
Templates and SOPs:
MSD:
Kit Vendor:
Sales representative: Romesh Draviam | RDraviam@meso-scale.com
Templates and SOPs:
ELISPOT Kit Vendors:
CCSG Shared Resource Cores:
Longitudinal Patient Collections for Clinical Trials:
Name | Role | Phone | Location | |
---|---|---|---|---|
ORION Core lab |
Oncology Research and Immunomonitoring
|
713-563-1955
|
ORIONCore@mdanderson.org
|
7435 Fannin St, 2SCR2.3219, Houston, TX 77054
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Service list |
► Cell Separation (2) | |||
Name | Description | Price | |
---|---|---|---|
Miltenyi Magnetic Bead Separation |
This service is for cell separation using magnetic beads and columns and includes single use reagents. Additional cost will be customized based upon the specific marker(s) for cell separation. |
Internal
$104.17
each
External in network $179.60 each External out of network $290.96 each UT-System $179.60 each Non-academic $303.53 each |
|
StemCell Magnetic Bead Separation |
This service is for cell separation using magnetic beads and includes single use reagents. Additional cost will be customized based upon the specific marker(s) for cell separation. |
Internal
$89.37
each
External in network $154.09 each External out of network $249.62 each UT-System $154.09 each |
|
► Flow Cytometry (6) | |||
Name | Description | Price | |
Small Flow Cytometry Panel/ Sample Processing and Staining |
Small flow panel include 6-9 fluorescent markers. We accept the sample type: Cryopreserved PBMC, Dissociated single cells and fresh tissue. Cryopreserved PBMC thawing and counting will require a separate service charge per sample. Fresh tissue will require an additional service charge per sample. *The sample is restricted to human biological specimen. |
Internal
$146.45
each
External out of network $409.05 each UT-System $252.50 each Non-academic $426.73 each |
|
Flow cytometry Analysis and Data Visualization (Hourly) |
MiFlowCyt report and Data analysis with Flowjo including graph of gated plot and statistic table. First sample should be applied the service fee of Flow Cytometry Compensation matrix. This cost will be applied hourly (2 samples/hour) |
Inquire | |
Flow Cytometry Compensation Matrix |
Compensation processing with single color control and FMO using flowjo |
Inquire | |
Flow Cytometry Panel Validation |
Cost will vary based on custom of antibody titration and panel development |
Inquire | |
Large Flow Cytometry Panel/ Sample processing and Staining |
Large flow panel include 10-18 fluorescent markers. We accept the sample type: Cryopreserved PBMC, Dissociated single cells and fresh tissue. Cryopreserved PBMC thawing and counting will require a separate service charge per sample. Fresh tissue will require an additional service charge per sample. *The sample is restricted to human biological specimen. |
Internal
$194.72
each
External out of network $543.87 each UT-System $335.72 each Non-academic $567.37 each |
|
PBMC QC Flow Cytometry Panel |
Small flow panel include 6-9 fluorescent markers. Batch analysis will require a separate 1hr service charge. |
Internal
$223.63
each
External in network $385.57 each External out of network $624.62 each UT-System $385.57 each Non-academic $651.61 each |
|
► Sample Handling (4) | |||
Name | Description | Price | |
Protein Quantification and Normalization |
BCA protein quantification and normalization of tissue lysates from Tissue Lysate preparation. One plate can run 40 tissue lysates in duplicate (Cost per plate) |
Internal
$110.39
plate
External in network $190.33 plate External out of network $308.33 plate UT-System $190.33 plate Non-academic $321.65 plate |
|
Tissue Lysate preparation |
Snap-frozen tissues may be submitted for tissue lysate preparation for downstream soluble factor analysis (Cost per sample). |
Inquire | |
Sample Thawing and Counting |
This service includes thawing cryopreserved cells, cell counting and viability assessment using either cellometer or hemocytometer |
Inquire | |
Sample Storage |
Short-term storage of samples prior to assay, charged per cryobox. |
Internal
$10.00
box
External in network $17.24 box External out of network $27.93 box UT-System $17.24 each Non-academic $29.14 each |