ALERT

On Thursday June 27th, 2019 at 5:00 AM (U.S. EST) Agilent Site Reliability Engineers will be working with our Network Service Provider to upgrade the API server for the North American instance. The entire process is expected to be 1 hour, and iLab customers using the API for any features may experience a decrease in performance during this time. More information can be found on the iLab Status Site. For any questions, please contact iLab Support at ilab-support@agilent.com or by clicking on the 'Help' link at the top of your account page.

MDACC Proteomics

Please Acknowledge

In order to continue obtaining Core support in a challenging environment, it is crucial that our users acknowledge the following grants in all publications (see following text).  Thank you!

 

This work was supported in part by Cancer Prevention Research Institute of Texas (CPRIT) grant number RP130397 and NIH grant numbers 1S10OD012304-01 and P30CA016672.

 

Overview of Services

Mission.  The mission of the Proteomics Core Facility is to provide the research community with cutting-edge expertise and services for sensitive, precise, and accurate identification and measurement of proteins using mass spectrometry-based technologies.

 

SERVICES

 

Proteomics:

  • Protein Identification: High sensitivity (silver stain OK), 1000 or more proteins may be indentifiable. 
  • Molecular Weight Determination: Check the MW of your peptide or molecule.  Small proteins may be measured if enough clean protein is available (larger proteins are less reliable), proteins are better analyzed by LC-MS (below). 
  • Quantitative Protein Analysis:  Using stable isotope methods, iTRAQ, TMT, SILAC, etc. For SRM/MRM methods on our tandem quadrupole MS, please inquire. Quantitative Protein Profiling using MRM, HRM or DIA (Swath-like) methods, with or without reference peptides (lists available, custom lists can be built).
  • Posttranslational Modification Analysis:  Phosphorylation, acetylation, methylation, etc. Please inquire for more detailed information.
  • LC or LC-MS Analysis:  Size exclusion chromatography and simple RP LC-MS are common examples.  Please inquire for more detailed information and other chromatographic analyses available.

 

 

 

Data Analysis:

  • Data Analysis: Many of the reports generated by our facility are time-consuming to assemble; we add a data analysis fee for reports that require an unusual amount of effort. Expertise includes bioinformatics, statistics, pathway analysis, and/or assistance preparing materials for manuscripts or presentations.

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Leadership 

 

Faculty Oversight:

Dr. Mien-Chie Hung

Dr. John Weinstein

 

Proteomic Services:

David Hawke, Ph.D., Director

Email:  dhawke@mdanderson.org

713.834.6096

  

Location and hours of operation

Hours: Location:

Sample Drop Off:

Monday - Friday

8 AM - 5 PM

By appointment only!                        

Lab: 2SCR3.3217
David Hawke: 2SCR3.3025             

  

Links and Resources

Our website contains additional information including an FAQ and tutorials.

  1. Proteomics Core Website
  2. Metabolomics Core Website

Contacts

Name Role Phone Email Location
David Hawke, Ph.D
Director
 
713-834-6096
 
dhawke@mdanderson.org
 
2SCR3.3025
 

Services & Rates


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Data Analysis (2)
Equipment Usage (1)
Proteomics (5)

Map


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