MDACC Advanced Microscopy Core

Overview of Services

Super-Resolution Structured Illumination Microscopy (SIM)

Equipment: OMX Blaze V4 Microscope

Super-Resolution Localization Microscopy (STORM/PALM/GSD)

Equipment: Vutara SR-200 Biplane Microscope (3-D) and OMX Blaze V4 Microscope (2-D)

Spatial resolution ~20 nm (XY), ~ 60 nm (Z)
Molecular studies of subcellular structures at the highest optical resolution available

Total Internal Reflection Microscopy (TIRF)

Equipment: OMX Blaze V4 Microscope

Spatial resolution ~250 nm (XY), ~ 100 nm (Z thickness)
Studies of live cell membrane receptor-ligand interactions, cellular adhesion, endo and exocytosis, etc.)

Förster/Fluorescence Resonance Energy Transfer (FRET)

Equipment: OMX Blaze V4 Microscope

Sensitized Emission (3-Cube) Method or Photobleaching Method

Laser Scanning Confocal Microscopy

Equipment: Leica SP8 Confocal Microscope with fast scanners, spectral detectors and live cell enclosure

Spatial resolution ~250 nm (XY), ~600 nm (Z)
Channel unmixing by spectral fingerprinting
Fast imaging of live or fixed cells and tissue by resonant scanning and photon-counting HyD detectors
FRAP (Fluorescence Recovery After Photobleaching) for molecule dynamics
Automated image stitching for large specimens and 3-D Assay imaging in multi-well plates

3-D Image Analysis and Visualization

IMARIS Software

3-D video design with rotations, fly-through, zooming and time-lapse visualization

Tomasz Zal|Core Director

M. Anna Zal|Core Manager

Hours	Location
Monday - Friday 8:00 am-5:00 pm	7455 Fannin Houston, TX 77054

Name	Role	Phone	Email	Location
M. Anna Zal, M.Sc.	Manager	713-563-7306	mazal@mdanderson.org	7455 Fannin Houston, TX 77054