AMC interim core facility director Dr. Collene Jeter (cjeter@mdanderson.org)
For assistance or training please contact Dr. Jeter or Anna Zal.
The new Zeiss Lightsheet 7 microscope has been installed and is available for pilot experiments. Contact Dr. Jeter for more info.
NEW: LS7 Meso and Micro-Scale Lightsheet Microscopy
Equipment: Zeiss Gaussian Lightsheet 7 (LS7) with 6 laser lines, 2 PCO Edge CMOS cameras and water and Clarity dipping objectives (2SCR3.3130)
Laser Scanning Confocal Microscopy with Spectral HyD Detectors
Equipment: Leica SP8 Confocal Microscope with fast scanners, spectral detectors and live cell enclosure (2SCR3.3130)
Super-Resolution Structured Illumination Microscopy (SIM)
Equipment: OMX Blaze V4 Microscope
Total Internal Reflection Microscopy (TIRF)
Equipment: OMX Blaze V4 Microscope
Fluorescence Recovery After Photobleaching (FRAP)
Equipment: Leica SP8 Confocal Microscope
Super-Resolution Localization Microscopy (STORM/PALM/GSD)
Equipment: OMX Blaze V4 Microscope (2-D)
Förster/Fluorescence Resonance Energy Transfer (FRET) Imaging
Equipment: OMX Blaze V4 Microscope
Image Analysis and Visualization
LCS3.x (Leica Confocal Software) (2SCR3.3130)
SoftWorx (OMX microscope)
IMARIS Software (2SCR3.3203)
Collene Jeter | Interim Core Director | cjeter@mdanderson.org| 832-750-7259 (office) & 512-694-0586 (cell)
M. Anna Zal |Core Manager | mazal@mdanderson.org| 713-563-7306 (office) & 346-234-0480 (cell)
Hours | Location |
Monday - Friday 8:00 am-5:00 pm |
2SCR3.3023 (office) 7455 Fannin, Houston, TX 77054 |
Name | Role | Phone | Location | |
---|---|---|---|---|
Collene Jeter |
Interim Director
|
832-750-7259
|
cjeter@mdanderson.org
|
1881 East Rd. Houston, TX 77054
|
M. Anna Zal |
Core Manager
|
713-563-7306
|
mazal@mdanderson.org
|
7455 Fannin, Houston, TX 77054
|
Service list |
► unclassified (30) | |||
Name | Description | Price | |
---|---|---|---|
Axio Observer (Epifluorescence) Assisted |
Hourly rate |
Inquire | |
Axio Observer (Epifluorescence) Independent |
Hourly rate; <30 min = no charge |
Inquire | |
Axio Observer Time-Lapse (>8 hours) |
Hourly rate (12 h max rate per day) |
Inquire | |
Axio Observer Training |
30 min; further assistance charged at Assisted rate |
Inquire | |
Deep Imaging Prep |
Sample dish + agarose 3D confocal or multiphoton imaging Per sample/dish |
Inquire | |
Imaging Consultation |
No charge |
Inquire | |
Imaris Image Processing Assisted |
Hourly rate |
Inquire | |
Imaris Image Processing Independent |
Hourly rate |
Inquire | |
Imaris Image Processing Training |
Hourly rate |
Inquire | |
INCell Assisted |
Hourly rate |
Inquire | |
INCell Training |
30 min; further assistance charge at Assisted rate |
Inquire | |
INCell Unassisted |
Slide/Plate scanning |
Inquire | |
LifeCanvas Active Clearing |
Detergent-based and electrophoretically enhanced whole organ clearing Consultation with core staff prior to sample preservation/submission required *SHIELD preservation REQUIRED May not be compatible with all tissue/organ types and/or epitopes |
Inquire | |
LifeCanvas Active IF Staining |
Electrophoretically enhanced whole organ IF staining Compatible with LifeCanvas validated antibodies; additional may be tested (see core staff) *Antibodies not provided as part of service fee Consultation with core staff prior to sample preservation/submission required May not be compatible with all tissue/organ types and/or epitopes |
Inquire | |
LifeCanvas Passive Tissue Clearing |
Detergent-based tissue clearing Consultation with core staff prior to sample preservation/submission required *SHIELD preservation recommended May not be compatible with all tissue/organ types and/or epitopes |
Inquire | |
LSM880 (Confocal) Assisted |
Hourly rate |
Inquire | |
LSM880 (Confocal) Independent |
Hourly rate |
Inquire | |
LSM880 (Confocal) Time-Lapse |
Available weekends (3 day maximum) Set up fee may be required
|
Inquire | |
LSM880 (Confocal) Training |
Hourly rate |
Inquire | |
Primary Antibody/Dye |
Per sample/coverslip |
Inquire | |
Standard Immunofluorescence Staining |
Per slide Core provided primary antibody/dye charged separately Additional fee for FFPE processing |
Inquire | |
Standard New Antibody Development |
6 sample maximum Confocal imaging charged separately as needed
|
Inquire | |
TCS SP8 (Multiphoton) Assisted |
Hourly rate |
Inquire | |
TCS SP8 (Multiphoton) Training |
With core management approval 4 hr miminum training; further assistance charged at Assisted rate |
Inquire | |
TCS SP8 (Multiphoton) Unassisted |
Requires prior TCS SP8 training and core management approval Can be added to assisted rate following sample set-up for long sample runs |
Inquire | |
Thick Tissue New Antibody Development |
6 sample maximum Multiphoton imaging charged separately |
Inquire | |
Thick Tissue Staining |
Core provided primary antibody/dye charged separately |
Inquire | |
Vibratome Sectioning Assisted |
Per sample 10 sections per sample maximum |
Inquire | |
Vibratome Sectioning Training |
Per hour
|
Inquire | |
Vibratome Sectioning Unassisted |
Per sample; 10 sections per sample maximum Subject to availability/approval |
Inquire |