Overview of Services
AMC interim core facility director Dr. Collene Jeter (cjeter@mdanderson.org)
For assistance or training please contact Dr. Jeter or Anna Zal.
The new Zeiss Lightsheet 7 microscope has been installed and is available for pilot experiments. Contact Dr. Jeter for more info.
NEW: LS7 Meso and Micro-Scale Lightsheet Microscopy
Equipment: Zeiss Gaussian Lightsheet 7 (LS7) with 6 laser lines, 2 PCO Edge CMOS cameras and water and Clarity dipping objectives (2SCR3.3130)
- Rapid volumetric imaging of fixed and cleared whole organs, tissues and embryos
- Dual sided lightsheet illumination
- 6 laser lines (405, 445, 488, 514, 561 and 638 nm) for rapid multispectral imaging from blue to far-red
- Optics include 5X and 20X water and Clarity (mixed immersion)
Laser Scanning Confocal Microscopy with Spectral HyD Detectors
Equipment: Leica SP8 Confocal Microscope with fast scanners, spectral detectors and live cell enclosure (2SCR3.3130)
- Fast imaging of live or fixed cells and tissues by resonant or conventional scanning
- Highest sensitivity photon-counting HyD detectors
- Spatial resolution ~250 nm (XY), ~600 nm (Z)
- Channel unmixing by spectral fingerprinting
- 3-D visualization
- FRAP (Fluorescence Recovery After Photobleaching) for molecule dynamics
- Automated image stitching for large specimens and 3-D Assay imaging in multi-well plates
Super-Resolution Structured Illumination Microscopy (SIM)
Equipment: OMX Blaze V4 Microscope
- Spatial resolution ~120 nm (XY), ~300 nm (Z)
- 3-D high impact imaging of cellular structures
Total Internal Reflection Microscopy (TIRF)
Equipment: OMX Blaze V4 Microscope
- Spatial resolution ~250 nm (XY), ~ 100 nm (Z thickness)
- Studies of live cell membrane receptor-ligand interactions, cellular adhesion, endo and exocytosis, etc.)
Fluorescence Recovery After Photobleaching (FRAP)
Equipment: Leica SP8 Confocal Microscope
- Dynamics of molecule trafficking in cells
Super-Resolution Localization Microscopy (STORM/PALM/GSD)
Equipment: OMX Blaze V4 Microscope (2-D)
- Spatial resolution ~20 nm (XY), ~ 60 nm (Z)
- Molecular studies of subcellular structures at the highest optical resolution available
Förster/Fluorescence Resonance Energy Transfer (FRET) Imaging
Equipment: OMX Blaze V4 Microscope
- 1-10 nm molecular distance sensitivity
- Static and dynamic mapping of molecular interactions inside cells
- ‘Cyan/Yellow’, ‘Green/Red’ and ‘Red/Far Red’ donor/acceptor compatibility
- Sensitized Emission (3-Cube) Method or Photobleaching Method
Image Analysis and Visualization
LCS3.x (Leica Confocal Software) (2SCR3.3130)
- Image pre-processing and basic analysis of images from Leica SP8 Confocal Microscope
- De-noising, spectral channel unmixing, distance measurement, z-stack projection (maximum intensity or average), 3-D rotation movie rendering, colocalization coefficients
SoftWorx (OMX microscope)
- Image pre-processing and basic analysis of images from OMX Blaze SIM Microscope
- De-noising, distance measurement, z-stack projection (maximum intensity or average), 3-D rotation movie rendering, colocalization coefficients, FRET, etc.
IMARIS Software (2SCR3.3203)
- High-end 3/4D Image Visualization and Analysis
- Adaptive thresholding with feature identification (spots, cells, nuclei, cytoplasm, cell membranes, neuronal dendrites, etc)
- Hundreds of morphological measurements
- Colocalization
- 3-D video design with rotations, fly-through, zooming and time-lapse visualization
- Spatiotemporal tracking
- Compatible with most existing microscope brands and image formats
Leadership
Collene Jeter | Interim Core Director | cjeter@mdanderson.org| 832-750-7259 (office) & 512-694-0586 (cell)
M. Anna Zal |Core Manager | mazal@mdanderson.org| 713-563-7306 (office) & 346-234-0480 (cell)
Location and hours of operation
| Hours |
Location |
|
Monday - Friday 8:00 am-5:00 pm
|
2SCR3.3023 (office)
7455 Fannin, Houston, TX 77054
|
Links and Resources
- MD Anderson Advanced Microscopy Core
Search available services:
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alphabetically
| Name |
Description |
Price |
|
Axio Observer (Epifluorescence) Assisted
|
Hourly rate
|
Inquire
|
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Axio Observer (Epifluorescence) Independent
|
Hourly rate; <30 min = no charge
|
Inquire
|
|
Axio Observer Time-Lapse (>8 hours)
|
Hourly rate (12 h max rate per day)
Set up fee may be required
Burner fee required for fluorescence imaging
|
Inquire
|
|
Axio Observer Training
|
30 min; further assistance charged at Assisted rate
|
Inquire
|
|
Deep Imaging Prep
|
Sample dish + agarose
3D confocal or multiphoton imaging
Per sample/dish
|
Inquire
|
|
Imaging Consultation
|
No charge
|
Inquire
|
|
Imaris Image Processing Assisted
|
Hourly rate
|
Inquire
|
|
Imaris Image Processing Independent
|
Hourly rate
|
Inquire
|
|
Imaris Image Processing Training
|
Hourly rate
|
Inquire
|
|
INCell Assisted
|
Hourly rate
|
Inquire
|
|
INCell Training
|
30 min; further assistance charge at Assisted rate
|
Inquire
|
|
INCell Unassisted
|
Slide/Plate scanning
|
Inquire
|
|
LifeCanvas Active Clearing
|
Detergent-based and electrophoretically enhanced whole organ clearing
Consultation with core staff prior to sample preservation/submission required *SHIELD preservation REQUIRED
May not be compatible with all tissue/organ types and/or epitopes
|
Inquire
|
|
LifeCanvas Active IF Staining
|
Electrophoretically enhanced whole organ IF staining
Compatible with LifeCanvas validated antibodies; additional may be tested (see core staff)
*Antibodies not provided as part of service fee
Consultation with core staff prior to sample preservation/submission required
May not be compatible with all tissue/organ types and/or epitopes
|
Inquire
|
|
LifeCanvas Passive Tissue Clearing
|
Detergent-based tissue clearing
Consultation with core staff prior to sample preservation/submission required *SHIELD preservation recommended
May not be compatible with all tissue/organ types and/or epitopes
|
Inquire
|
|
LSM880 (Confocal) Assisted
|
Hourly rate
|
Inquire
|
|
LSM880 (Confocal) Independent
|
Hourly rate
|
Inquire
|
|
LSM880 (Confocal) Time-Lapse
|
Available weekends (3 day maximum)
Set up fee may be required
|
Inquire
|
|
LSM880 (Confocal) Training
|
Hourly rate
|
Inquire
|
|
Primary Antibody/Dye
|
Per sample/coverslip
|
Inquire
|
|
Standard Immunofluorescence Staining
|
Per slide
Core provided primary antibody/dye charged separately
Additional fee for FFPE processing
|
Inquire
|
|
Standard New Antibody Development
|
6 sample maximum
Confocal imaging charged separately as needed
|
Inquire
|
|
TCS SP8 (Multiphoton) Assisted
|
Hourly rate
|
Inquire
|
|
TCS SP8 (Multiphoton) Training
|
With core management approval
4 hr miminum training; further assistance charged at Assisted rate
|
Inquire
|
|
TCS SP8 (Multiphoton) Unassisted
|
Requires prior TCS SP8 training and core management approval
Can be added to assisted rate following sample set-up for long sample runs
|
Inquire
|
|
Thick Tissue New Antibody Development
|
6 sample maximum
Multiphoton imaging charged separately
|
Inquire
|
|
Thick Tissue Staining
|
Core provided primary antibody/dye charged separately
|
Inquire
|
|
Vibratome Sectioning Assisted
|
Per sample
10 sections per sample maximum
|
Inquire
|
|
Vibratome Sectioning Training
|
Per hour
|
Inquire
|
|
Vibratome Sectioning Unassisted
|
Per sample; 10 sections per sample maximum
Subject to availability/approval
|
Inquire
|
|
▼
►
unclassified (30)
|
| Name |
Description |
Price |
|
Axio Observer (Epifluorescence) Assisted
|
Hourly rate
|
Inquire
|
|
Axio Observer (Epifluorescence) Independent
|
Hourly rate; <30 min = no charge
|
Inquire
|
|
Axio Observer Time-Lapse (>8 hours)
|
Hourly rate (12 h max rate per day)
Set up fee may be required
Burner fee required for fluorescence imaging
|
Inquire
|
|
Axio Observer Training
|
30 min; further assistance charged at Assisted rate
|
Inquire
|
|
Deep Imaging Prep
|
Sample dish + agarose
3D confocal or multiphoton imaging
Per sample/dish
|
Inquire
|
|
Imaging Consultation
|
No charge
|
Inquire
|
|
Imaris Image Processing Assisted
|
Hourly rate
|
Inquire
|
|
Imaris Image Processing Independent
|
Hourly rate
|
Inquire
|
|
Imaris Image Processing Training
|
Hourly rate
|
Inquire
|
|
INCell Assisted
|
Hourly rate
|
Inquire
|
|
INCell Training
|
30 min; further assistance charge at Assisted rate
|
Inquire
|
|
INCell Unassisted
|
Slide/Plate scanning
|
Inquire
|
|
LifeCanvas Active Clearing
|
Detergent-based and electrophoretically enhanced whole organ clearing
Consultation with core staff prior to sample preservation/submission required *SHIELD preservation REQUIRED
May not be compatible with all tissue/organ types and/or epitopes
|
Inquire
|
|
LifeCanvas Active IF Staining
|
Electrophoretically enhanced whole organ IF staining
Compatible with LifeCanvas validated antibodies; additional may be tested (see core staff)
*Antibodies not provided as part of service fee
Consultation with core staff prior to sample preservation/submission required
May not be compatible with all tissue/organ types and/or epitopes
|
Inquire
|
|
LifeCanvas Passive Tissue Clearing
|
Detergent-based tissue clearing
Consultation with core staff prior to sample preservation/submission required *SHIELD preservation recommended
May not be compatible with all tissue/organ types and/or epitopes
|
Inquire
|
|
LSM880 (Confocal) Assisted
|
Hourly rate
|
Inquire
|
|
LSM880 (Confocal) Independent
|
Hourly rate
|
Inquire
|
|
LSM880 (Confocal) Time-Lapse
|
Available weekends (3 day maximum)
Set up fee may be required
|
Inquire
|
|
LSM880 (Confocal) Training
|
Hourly rate
|
Inquire
|
|
Primary Antibody/Dye
|
Per sample/coverslip
|
Inquire
|
|
Standard Immunofluorescence Staining
|
Per slide
Core provided primary antibody/dye charged separately
Additional fee for FFPE processing
|
Inquire
|
|
Standard New Antibody Development
|
6 sample maximum
Confocal imaging charged separately as needed
|
Inquire
|
|
TCS SP8 (Multiphoton) Assisted
|
Hourly rate
|
Inquire
|
|
TCS SP8 (Multiphoton) Training
|
With core management approval
4 hr miminum training; further assistance charged at Assisted rate
|
Inquire
|
|
TCS SP8 (Multiphoton) Unassisted
|
Requires prior TCS SP8 training and core management approval
Can be added to assisted rate following sample set-up for long sample runs
|
Inquire
|
|
Thick Tissue New Antibody Development
|
6 sample maximum
Multiphoton imaging charged separately
|
Inquire
|
|
Thick Tissue Staining
|
Core provided primary antibody/dye charged separately
|
Inquire
|
|
Vibratome Sectioning Assisted
|
Per sample
10 sections per sample maximum
|
Inquire
|
|
Vibratome Sectioning Training
|
Per hour
|
Inquire
|
|
Vibratome Sectioning Unassisted
|
Per sample; 10 sections per sample maximum
Subject to availability/approval
|
Inquire
|
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