Overview of Services
Super-Resolution Structured Illumination Microscopy (SIM)
Equipment: OMX Blaze V4 Microscope
- Spatial resolution ~120 nm (XY), ~300 nm (Z)
- 3-D high impact imaging of cellular structures
Super-Resolution Localization Microscopy (STORM/PALM/GSD)
Equipment: Vutara SR-200 Biplane Microscope (3-D) and OMX Blaze V4 Microscope (2-D)
- Spatial resolution ~20 nm (XY), ~ 60 nm (Z)
- Molecular studies of subcellular structures at the highest optical resolution available
Total Internal Reflection Microscopy (TIRF)
Equipment: OMX Blaze V4 Microscope
- Spatial resolution ~250 nm (XY), ~ 100 nm (Z thickness)
- Studies of live cell membrane receptor-ligand interactions, cellular adhesion, endo and exocytosis, etc.)
Förster/Fluorescence Resonance Energy Transfer (FRET) Imaging
Equipment: OMX Blaze V4 Microscope, Intelligent Imaging Innovations Dual Camera Nikon Eclipse Ti Microscope
- 1-10 nm molecular distance sensitivity
- Static and dynamic mapping of molecular interactions inside cells
- ‘Cyan/Yellow’, ‘Green/Red’ and ‘Red/Far Red’ donor/acceptor compatibility
Sensitized Emission (3-Cube) Method or Photobleaching Method
Laser Scanning Confocal Microscopy
Equipment: Leica SP8 Confocal Microscope with fast scanners, spectral detectors and live cell enclosure
- Fast imaging of live or fixed cells and tissues by resonant scanning and photon-counting HyD detectors
- Spatial resolution ~250 nm (XY), ~600 nm (Z)
- Channel unmixing by spectral fingerprinting
- FRAP (Fluorescence Recovery After Photobleaching) for molecule dynamics
- Automated image stitching for large specimens and 3-D Assay imaging in multi-well plates
Intravital Multiphoton (2-Photon) Microscopy
Equipment: Custom Leica SP5MP Microscope with dual MaiTai fs Lasers, 4-channel non-descanned detection, photon-counting HyD sensors and small animal life support
- Extensive expertise in intravital tumor microscopy including in skin, lung, brain, pancreas, liver, bone, intestine and lymph nodes.
- NA 1.10 water immersion objective
- FLIM and PLIM (fluorescence and phosphorescence lifetime imaging microscopy) using Becker&Hickl sub-system
- Availability of fluorescence reporter mouse strains
3-D Image Analysis and Visualization
IMARIS Software
- Adaptive thresholding with hundreds of morphological measurements
- Spatiotemporal tracking
- 3-D video design with rotations, fly-through, zooming and time-lapse visualization
Leadership
Tomasz Zal|Core Director
M. Anna Zal|Core Manager
Location and hours of operation
| Hours |
Location |
|
Monday - Friday 8:00 am-5:00 pm
|
7455 Fannin
Houston, TX 77054
|
Links and Resources
- MD Anderson Advanced Microscopy Core
Welcome!
