Our location: South Campus 2SCR3.3023 (office)
Overview of Services
Laser Scanning Confocal Microscopy with Spectral HyD Detectors
Equipment: Leica SP8 Confocal Microscope with fast scanners, spectral detectors and live cell enclosure (2SCR3.3130)
- Fast imaging of live or fixed cells and tissues by resonant or conventional scanning
- Highest sensitivity photon-counting HyD detectors
- Spatial resolution ~250 nm (XY), ~600 nm (Z)
- Channel unmixing by spectral fingerprinting
- 3-D visualization
- FRAP (Fluorescence Recovery After Photobleaching) for molecule dynamics
- Automated image stitching for large specimens and 3-D Assay imaging in multi-well plates
Intravital Multiphoton (2-Photon) Microscopy
Equipment: Custom Leica SP5MP Microscope with dual MaiTai fs Lasers, 2x4-channel non-descanned detection, Becker&Hickl FLIM/PLIM, and small animal life support (2SCR3.3123)
- Dynamic imaging of cellular behavior in vivo
- Extensive expertise in intravital tumor microscopy including in skin, lung, brain, pancreas, liver, bone, intestine and lymph nodes
- Immune surveillance
- Cancer metastasis and invasion
- Thick specimen imaging - fresh tissues, tumor spheroids, tissue phantoms, clarified tissues
- Therapeutic mechanisms
- Up to 8 color channels by dual laser excitation multiplexing
- Two photon FLIM and PLIM (fluorescence and phosphorescence lifetime imaging microscopy)
- Project/grant collaboration
Super-Resolution Structured Illumination Microscopy (SIM)
Equipment: OMX Blaze V4 Microscope
- Spatial resolution ~120 nm (XY), ~300 nm (Z)
- 3-D high impact imaging of cellular structures
Super-Resolution Localization Microscopy (STORM/PALM/GSD)
Equipment: Vutara SR-200 Biplane Microscope (3-D) and OMX Blaze V4 Microscope (2-D)
- Spatial resolution ~20 nm (XY), ~ 60 nm (Z)
- Molecular studies of subcellular structures at the highest optical resolution available
Total Internal Reflection Microscopy (TIRF)
Equipment: OMX Blaze V4 Microscope
- Spatial resolution ~250 nm (XY), ~ 100 nm (Z thickness)
- Studies of live cell membrane receptor-ligand interactions, cellular adhesion, endo and exocytosis, etc.)
Förster/Fluorescence Resonance Energy Transfer (FRET) Imaging
Equipment: OMX Blaze V4 Microscope, Intelligent Imaging Innovations Dual Camera Nikon Eclipse Ti Microscope
- 1-10 nm molecular distance sensitivity
- Static and dynamic mapping of molecular interactions inside cells
- ‘Cyan/Yellow’, ‘Green/Red’ and ‘Red/Far Red’ donor/acceptor compatibility
- Sensitized Emission (3-Cube) Method or Photobleaching Method
Image Analysis and Visualization
LCS3.x (Leica Confocal Software) (2SCR3.3130)
- Image pre-processing and basic analysis of images from Leica SP8 Confocal Microscope
- De-noising, spectral channel unmixing, distance measurement, z-stack projection (maximum intensity or average), 3-D rotation movie rendering, colocalization coefficients
SoftWorx (OMX microscope)
- Image pre-processing and basic analysis of images from OMX Blaze SIM Microscope
- De-noising, distance measurement, z-stack projection (maximum intensity or average), 3-D rotation movie rendering, colocalization coefficients, FRET, etc.
IMARIS Software (2SCR3.3203)
- High-end 3/4D Image Visualization and Analysis
- Adaptive thresholding with feature identification (spots, cells, nuclei, cytoplasm, cell membranes, neuronal dendrites, etc)
- Hundreds of morphological measurements
- Colocalization
- 3-D video design with rotations, fly-through, zooming and time-lapse visualization
- Spatiotemporal tracking
- Compatible with most existing microscope brands and image formats
Leadership
Tomasz Zal | Core Director
M. Anna Zal | Core Manager | mazal@mdanderson.org | tel. 713-563-7306
Location and hours of operation
| Hours |
Location |
|
Monday - Friday 8:00 am-5:00 pm
|
2SCR3.3023 (office)
7455 Fannin, Houston, TX 77054
|
Links and Resources
- MD Anderson Advanced Microscopy Core
Welcome!
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