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MDACC Advanced Microscopy Core

Our location: South Campus 2SCR3.3023 (office)

Overview of Services

Laser Scanning Confocal Microscopy with Spectral HyD Detectors

Equipment: Leica SP8 Confocal Microscope with fast scanners, spectral detectors and live cell enclosure (2SCR3.3130)

  • Fast imaging of live or fixed cells and tissues by resonant or conventional scanning
  • Highest sensitivity photon-counting HyD detectors
  • Spatial resolution ~250 nm (XY), ~600 nm (Z)
  • Channel unmixing by spectral fingerprinting
  • 3-D visualization
  • FRAP (Fluorescence Recovery After Photobleaching) for molecule dynamics
  • Automated image stitching for large specimens and 3-D Assay imaging in multi-well plates

Intravital Multiphoton (2-Photon) Microscopy

Equipment: Custom Leica SP5MP Microscope with dual MaiTai fs Lasers, 2x4-channel non-descanned detection, Becker&Hickl FLIM/PLIM, and small animal life support (2SCR3.3123)

  • Dynamic imaging of cellular behavior in vivo
  • Extensive expertise in intravital tumor microscopy including in skin, lung, brain, pancreas, liver, bone, intestine and lymph nodes
  • Immune surveillance
  • Cancer metastasis and invasion
  • Thick specimen imaging - fresh tissues, tumor spheroids, tissue phantoms, clarified tissues
  • Therapeutic mechanisms
  • Up to 8 color channels by dual laser excitation multiplexing
  • Two photon FLIM and PLIM (fluorescence and phosphorescence lifetime imaging microscopy)
  • Project/grant collaboration

Super-Resolution Structured Illumination Microscopy (SIM)

Equipment: OMX Blaze V4 Microscope

  • Spatial resolution ~120 nm (XY), ~300 nm (Z)
  • 3-D high impact imaging of cellular structures

Super-Resolution Localization Microscopy (STORM/PALM/GSD)

Equipment: Vutara SR-200 Biplane Microscope (3-D) and OMX Blaze V4 Microscope (2-D)

  • Spatial resolution ~20 nm (XY), ~ 60 nm (Z)
  • Molecular studies of subcellular structures at the highest optical resolution available

Total Internal Reflection Microscopy (TIRF)

Equipment: OMX Blaze V4 Microscope  

  • Spatial resolution ~250 nm (XY), ~ 100 nm (Z thickness)
  • Studies of live cell membrane receptor-ligand interactions, cellular adhesion, endo and exocytosis, etc.)

Förster/Fluorescence Resonance Energy Transfer (FRET) Imaging

Equipment: OMX Blaze V4 Microscope, Intelligent Imaging Innovations Dual Camera Nikon Eclipse Ti Microscope

  • 1-10 nm molecular distance sensitivity
  • Static and dynamic mapping of molecular interactions inside cells
  • ‘Cyan/Yellow’, ‘Green/Red’ and ‘Red/Far Red’ donor/acceptor compatibility
  • Sensitized Emission (3-Cube) Method or Photobleaching Method

Image Analysis and Visualization

LCS3.x (Leica Confocal Software) (2SCR3.3130)

  • Image pre-processing and basic analysis of images from Leica SP8 Confocal Microscope
  • De-noising, spectral channel unmixing, distance measurement, z-stack projection (maximum intensity or average), 3-D rotation movie rendering, colocalization coefficients

SoftWorx (OMX microscope)

  • Image pre-processing and basic analysis of images from OMX Blaze SIM Microscope
  • De-noising, distance measurement, z-stack projection (maximum intensity or average), 3-D rotation movie rendering, colocalization coefficients, FRET, etc.

IMARIS Software (2SCR3.3203)

  • High-end 3/4D Image Visualization and Analysis
  • Adaptive thresholding with feature identification (spots, cells, nuclei, cytoplasm, cell membranes, neuronal dendrites, etc)
  • Hundreds of morphological measurements
  • Colocalization
  • 3-D video design with rotations, fly-through, zooming and time-lapse visualization
  • Spatiotemporal tracking
  • Compatible with most existing microscope brands and image formats

Leadership

Tomasz Zal | Core Director

M. Anna Zal | Core Manager | mazal@mdanderson.org | tel. 713-563-7306

Location and hours of operation

Hours      Location

Monday - Friday 8:00 am-5:00 pm 

     2SCR3.3023 (office)

7455 Fannin, Houston, TX 77054

Links and Resources

  1. MD Anderson Advanced Microscopy Core

Contacts

Name Role Phone Email Location
M. Anna Zal, M.Sc.
Manager
 
713-563-7306
 
mazal@mdanderson.org
 
7455 Fannin Houston, TX 77054
 

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