MDACC Advanced Microscopy Core

Overview of Services

Super-Resolution Structured Illumination Microscopy (SIM)

Equipment: OMX Blaze V4 Microscope

  • Spatial resolution ~120 nm (XY), ~300 nm (Z)
  • 3-D high impact imaging of cellular structures

Super-Resolution Localization Microscopy (STORM/PALM/GSD)

Equipment: Vutara SR-200 Biplane Microscope (3-D) and OMX Blaze V4 Microscope (2-D)

  • Spatial resolution ~20 nm (XY), ~ 60 nm (Z)
  • Molecular studies of subcellular structures at the highest optical resolution available

Total Internal Reflection Microscopy (TIRF)

Equipment: OMX Blaze V4 Microscope  

  • Spatial resolution ~250 nm (XY), ~ 100 nm (Z thickness)
  • Studies of live cell membrane receptor-ligand interactions, cellular adhesion, endo and exocytosis, etc.)

Förster/Fluorescence Resonance Energy Transfer (FRET) Imaging

Equipment: OMX Blaze V4 Microscope, Intelligent Imaging Innovations Dual Camera Nikon Eclipse Ti Microscope

  • 1-10 nm molecular distance sensitivity
  • Static and dynamic mapping of molecular interactions inside cells
  • ‘Cyan/Yellow’, ‘Green/Red’ and ‘Red/Far Red’ donor/acceptor compatibility

Sensitized Emission (3-Cube) Method or Photobleaching Method

Laser Scanning Confocal Microscopy

Equipment: Leica SP8 Confocal Microscope with fast scanners, spectral detectors and live cell enclosure

  • Fast imaging of live or fixed cells and tissues by resonant scanning and photon-counting HyD detectors
  • Spatial resolution ~250 nm (XY), ~600 nm (Z)
  • Channel unmixing by spectral fingerprinting
  • FRAP (Fluorescence Recovery After Photobleaching) for molecule dynamics
  • Automated image stitching for large specimens and 3-D Assay imaging in multi-well plates

Intravital Multiphoton (2-Photon) Microscopy

Equipment: Custom Leica SP5MP Microscope with dual MaiTai fs Lasers, 4-channel non-descanned detection, photon-counting HyD sensors and small animal life support

  • Extensive expertise in intravital tumor microscopy including in skin, lung, brain, pancreas, liver, bone, intestine and lymph nodes.
  • NA 1.10 water immersion objective
  • FLIM and PLIM (fluorescence and phosphorescence lifetime imaging microscopy) using Becker&Hickl sub-system
  • Availability of fluorescence reporter mouse strains

3-D Image Analysis and Visualization

IMARIS Software

  • Adaptive thresholding with hundreds of morphological measurements
  • Spatiotemporal tracking
  • 3-D video design with rotations, fly-through, zooming and time-lapse visualization

 

Leadership

Tomasz Zal|Core Director

M. Anna Zal|Core Manager

Location and hours of operation

Hours      Location

Monday - Friday 8:00 am-5:00 pm 

     7455 Fannin
     Houston, TX 77054

Links and Resources

  1. MD Anderson Advanced Microscopy Core

Contacts

Name Role Phone Email Location
M. Anna Zal, M.Sc.
Manager
 
713-563-7306
 
mazal@mdanderson.org
 
7455 Fannin Houston, TX 77054
 

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