Please Acknowledge

In order to continue obtaining Core support in a challenging environment, it is crucial that our users acknowledge the following grants in all publications (see following text).  Thank you!

“This work was supported in part by Cancer Prevention Research Institute of Texas (CPRIT) grant number RP130397 and NIH grant numbers 1S10OD012304-01 and P30CA016672.”

Overview of Services

Mission.  The mission of the Metabolomics Core Facility is to provide the research community with cutting-edge expertise and services for sensitive, precise, and accurate measurement of metabolites using mass spectrometry-based technologies.

SERVICES

Metabolomics: 

      Targeted

• Amino Acids (30): Absolute quantitation of the 18 proteogenic amino acids and 12 related molecules. (See list below)
• Organic Acids (8): Absolute quantitation of 8 organic acids. (See list below)
• Glutathione (2): Absolute quantitation of reduced (GSH) and oxidized (GSSG) glutathione.
• Polyamines (14): Absolute quantitation of 14 polyamines. (See list below)
• DNA Damage Metabolites (6): Absolute quantitation of 6 DNA damage metabolites. (See list below)
• Anionic Metabolites by IC-MS (80): Relative quantitation of metabolites associated primarily with glycolysis, TCA cycle, and pentose phosphate pathway.
• Stable Isotope Tracer Analysis: Measurement of stable isotope incorporation into targeted metabolites (assays above) downstream of a chosen tracer metabolite. Multiplexed, multi-tracer analysis is available.

     Non-targeted

• Profiling (complete): Relative quantitation of metabolites from multiple fractions.
• Lipidomic Profiling: Relative quantitation of multiple classes of lipid metabolites from nonpolar fractions.
• Hyaluronic Acid Profiling: Relative quantitation of hyaluronic acid oligomers.
• Tracer Analysis: Measurement of stable isotope incorporation into non-targeted metabolites downstream of a chosen tracer metabolite.  Multiplexed, multi-tracer analysis is available.

     Other Services

• Redox Metabolism Assays: Relative quantitation of ROS (H2O2) using a microplate (96-well) assay.
• Mechanism of Action Studies: Assistance with pharmacological experiments to determine an agent's mechanism of action by integrating pharmacological endpoints (e.g., cell viability) with metabolomic and other omic data.
• Sample Preparation Assistance: Assistance with or full-service provision of sample preparation. For example, you can ship cells and treatment(s), we can work together to design an experiment, and we can conduct the entire experiment.

Data Analysis:

• Data Analysis: Many of the reports generated by our facility are time-consuming to assemble; we add a data analysis fee of $250 for reports that require 1-4 h of effort. Expertise includes bioinformatics, statistics, pathway analysis, and/or assistance preparing materials for manuscripts or presentations. Many data types accepted (genomic, proteomic, metabolomic, pharmacological, etc.).
• Drug Combination Analysis: We have developed software for conducting 3-D response surface and response envelope analyses of drug combinations for the statistical determination of synergy, additivity, or antagonism. (Please contact Phil Lorenzi to discuss)

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 AMINO ACIDS: 

ORGANIC ACIDS: 

POLYAMINES:

DNA DAMAGE METABOLITES:

Leadership 

Faculty Oversight:

Dr. Mien-Chie Hung

Dr. John Weinstein

Metabolomics Services:

Phil Lorenzi, Ph.D., Co-Director

Email: PLLorenzi@mdanderson.org

713.792.9999

Location and hours of operation

Links and Resources

Our website contains additional information including an FAQ and tutorials.

1. Proteomics and Metabolomics Core Website
2. MDACC Proteomics

Contacts