MDACC Metabolomics

Please Acknowledge

In order to continue obtaining Core support in a challenging environment, it is crucial that our users acknowledge the following grants in all publications (see following text). Thank you!

“This work was supported in part by Cancer Prevention Research Institute of Texas (CPRIT) grant number RP130397 and NIH grant numbers 1S10OD012304-01 and P30CA016672.”

Overview of Services

Mission. The mission of the Metabolomics Core Facility is to provide the research community with cutting-edge expertise and services for sensitive, precise, and accurate measurement of metabolites using mass spectrometry-based technologies.

SERVICES

Metabolomics:

Targeted

Amino Acids (30): Absolute quantitation of the 18 proteogenic amino acids and 12 related molecules. (See list below)
Organic Acids (8): Absolute quantitation of 8 organic acids. (See list below)
Glutathione (2): Absolute quantitation of reduced (GSH) and oxidized (GSSG) glutathione.
Polyamines (14): Absolute quantitation of 14 polyamines. (See list below)
DNA Damage Metabolites (6): Absolute quantitation of 6 DNA damage metabolites. (See list below)
Anionic Metabolites by IC-MS (80): Relative quantitation of metabolites associated primarily with glycolysis, TCA cycle, and pentose phosphate pathway.
Stable Isotope Tracer Analysis: Measurement of stable isotope incorporation into targeted metabolites (assays above) downstream of a chosen tracer metabolite. Multiplexed, multi-tracer analysis is available.

Non-targeted

Profiling (complete): Relative quantitation of metabolites from multiple fractions.
Lipidomic Profiling: Relative quantitation of multiple classes of lipid metabolites from nonpolar fractions.
Hyaluronic Acid Profiling: Relative quantitation of hyaluronic acid oligomers.
Tracer Analysis: Measurement of stable isotope incorporation into non-targeted metabolites downstream of a chosen tracer metabolite. Multiplexed, multi-tracer analysis is available.

Other Services

Redox Metabolism Assays: Relative quantitation of ROS (H2O2) using a microplate (96-well) assay.
Mechanism of Action Studies: Assistance with pharmacological experiments to determine an agent's mechanism of action by integrating pharmacological endpoints (e.g., cell viability) with metabolomic and other omic data.
Sample Preparation Assistance: Assistance with or full-service provision of sample preparation. For example, you can ship cells and treatment(s), we can work together to design an experiment, and we can conduct the entire experiment.

Data Analysis:

Data Analysis: Many of the reports generated by our facility are time-consuming to assemble; we add a data analysis fee of $250 for reports that require 1-4 h of effort. Expertise includes bioinformatics, statistics, pathway analysis, and/or assistance preparing materials for manuscripts or presentations. Many data types accepted (genomic, proteomic, metabolomic, pharmacological, etc.).
Drug Combination Analysis: We have developed software for conducting 3-D response surface and response envelope analyses of drug combinations for the statistical determination of synergy, additivity, or antagonism. (Please contact Phil Lorenzi to discuss)

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AMINO ACIDS:

1	4-hydroxy-proline	16	isoleucine (Ile)
2	acetylcholine	17	leucine (Leu)
3	alanine (Ala)	18	lysine (Lys)
4	arginine (Arg)	19	methionine (Met)
5	asparagine (Asn)	20	S-adenosylhomocysteine (SAH)
6	carnitine	21	ornithine
7	citrulline	22	phenylalanine (Phe)
8	creatine	23	proline (Pro)
9	creatinine	24	sarcosine
10	cysteine (Cys)	25	serine (Ser)
11	cystine (Cys-Cys disulfide)	26	taurine
12	gamma-aminobutyric acid (GABA)	27	threonine (Thr)
13	glutamine (Gln)	28	tryptophan (Trp)
14	glycine (Gly)	29	tyrosine (Tyr)
15	histidine (His)	30	valine (Val)

ORGANIC ACIDS:

1	glutamate (Glu)
2	glutamine (Gln)
3	aspartate (Asp)
4	alpha-ketoglutarate (a-KG)
5	2-hydroxyglutarate (2-HG)
6	pyruvate
7	lactate
8	pyroglutamic acid (pGlu)

POLYAMINES:

1	tyramine	8	putrescine
2	tryptamine	9	agmatine
3	N-acetylputrescine	10	hypusine
4	diacetylspermine	11	diaminopropane
5	ornithine	12	N-acetylspermine
6	diaminohexane	13	spermidine
7	cadaverine	14	spermine

DNA DAMAGE METABOLITES:

1	NAD(+)
2	AMP
3	NADH
4	ADP-ribose
5	ADP
6	ATP

Leadership

Faculty Oversight:

Dr. Mien-Chie Hung

Dr. John Weinstein

Metabolomics Services:

Phil Lorenzi, Ph.D., Co-Director

Email: PLLorenzi@mdanderson.org

713.792.9999

Location and hours of operation

Hours:

Location:

Sample Drop Off:

Monday - Friday

7 AM - 5 PM

By appointment only!

Lab: 2SCR3.3217
Phil Lorenzi: 2SCR3.3029

Links and Resources

Our website contains additional information including an FAQ and tutorials.

Contacts

Name	Role	Phone	Email	Location
Phil Lorenzi, Ph.D	Co-Director	713-792-9999	PLLorenzi@mdanderson.org	2SCR3.3029

Services & Rates

Name	Description	Price
Advanced Data Analysis (per hour)		Inquire
Amino Acids (Targeted)	Approximately 30 amino acids and related metabolites.	Inquire
Bile Acids (Targeted)	Targeted assay for the measurement of 18 bile acids	Inquire
CoAs	Optimized method for the measurement of 3 coenzyme A (CoA) species.	Inquire
Data Analysis (External Data e.g., genomics, RPPA, microarray, sequencing, drug combination effects, etc; per hour)		Inquire
digital PCR-instrument time and consumables (per plate of up to 16 samples)	Applied Biosystems (Thermo) Absolute Q digital PCR system. Cost is for instrument time and consumables for 1 plate (16 wells). Please bring your own DNA and primers/TaqMan probes.	Non-Academic $598.69 each Internal $300.00 each External in network $319.15 each External out of network $517.02 each UT-System $319.15 each
Equipment Usage (Metabolomics)	Fee is Per Hour with a minimum usage of 1 hour	Inquire
Fatty Acids, Medium- and Long-Chain, Free		Inquire
Fatty Acids, Medium- and Long-Chain, Total		Inquire
Full Service Experiment - Setup and Sample Preparation		Inquire
HILIC-MS	Approximately 300 polar metabolites	Inquire
IC-MS Anionic Metabolites (Targeted)	Approximately 150 anionic metabolites including glycolysis, TCA cycle, pentose phosphate pathway, and nucleotides.	Inquire
L-, D-2-HG	Chiral separation and quantitation of L- and D-2-hydroxyglutarate by LC-MS/MS.	Inquire
Lipidomic Profiling		Inquire
New Assay Setup (Per Day)	Don't see the molecule you want to measure listed on our menu? We can probably add it to one of our existing assays or build a new method. Most molecules can be added with 2-3 days of labor, which includes optimization of a range of parameters including extraction/recovery. Challenging biological matrices (e.g., stool) often require more time for optimization.	Inquire
NULISA - Inflammation Panel (human)	250 cytokines, chemokines, inflammation markers measured with ultra-high sensitivity	Inquire
Patient Sample Aliquot	Aliquot patient liquid samples to avoid future freeze/thaw for clients. Price is for one sample (one tube of original sample).	Inquire
Polyamines (Targeted)	Approximately 14 polyamines.	Inquire
ProteinSimple Peggy Sue Nanoimmunoassay (size or charge) - Instrument Use	Walk-up access to Peggy Sue for trained users who provide their own reagents and labor. Price is for single-day use. For full service nanoimmunoassays, see Proteomic Services menu.	Inquire
Seahorse XFe96 Flux Analyzer - full service	Seahorse assay (ATP production rate, glycolytic rate, etc.). Cost is for instrument time + consumables (1 assay) + labor (provided by core facility staff).	Inquire
Seahorse XFe96 Flux Analyzer - instrument time and consumables	Seahorse assay (ATP production rate, glycolytic rate, etc.). Cost is for core staff to provide consumables for 1 assay (plate) and instrument time.	Inquire
Seahorse XFe96 Flux Analyzer - Instrument Use	Walk-up access to Seahorse XFe96 Flux Analyzer for trained users who provide their own reagents and labor. Cost is for single-day use. For full service Seahorse XFe96 Flux Analysis, see Metabolomic Services menu.	Inquire
Stable Isotope Tracer Analysis (Non-targeted)		Inquire
Stable Isotope Tracer Analysis (Targeted)	We can generally trace any substrate the client desires. Examples include: 13C6-glucose, 1,2-13C2-glucose, 13C3-lactate, 13C3-pyruvate, 13C5-glutamine, 13C16-palmitate, 13C2-acetate, 15N1-glutamine.	Inquire
Targeted analysis (custom)		Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Metabolomics (17)
New Assay Setup (Per Day)	Don't see the molecule you want to measure listed on our menu? We can probably add it to one of our existing assays or build a new method. Most molecules can be added with 2-3 days of labor, which includes optimization of a range of parameters including extraction/recovery. Challenging biological matrices (e.g., stool) often require more time for optimization.	Inquire
IC-MS Anionic Metabolites (Targeted)	Approximately 150 anionic metabolites including glycolysis, TCA cycle, pentose phosphate pathway, and nucleotides.	Inquire
HILIC-MS	Approximately 300 polar metabolites	Inquire
Lipidomic Profiling		Inquire
Fatty Acids, Medium- and Long-Chain, Free		Inquire
Fatty Acids, Medium- and Long-Chain, Total		Inquire
Amino Acids (Targeted)	Approximately 30 amino acids and related metabolites.	Inquire
Polyamines (Targeted)	Approximately 14 polyamines.	Inquire
CoAs	Optimized method for the measurement of 3 coenzyme A (CoA) species.	Inquire
L-, D-2-HG	Chiral separation and quantitation of L- and D-2-hydroxyglutarate by LC-MS/MS.	Inquire
Bile Acids (Targeted)	Targeted assay for the measurement of 18 bile acids	Inquire
Stable Isotope Tracer Analysis (Targeted)	We can generally trace any substrate the client desires. Examples include: 13C6-glucose, 1,2-13C2-glucose, 13C3-lactate, 13C3-pyruvate, 13C5-glutamine, 13C16-palmitate, 13C2-acetate, 15N1-glutamine.	Inquire
Stable Isotope Tracer Analysis (Non-targeted)		Inquire
Targeted analysis (custom)		Inquire
Seahorse XFe96 Flux Analyzer - instrument time and consumables	Seahorse assay (ATP production rate, glycolytic rate, etc.). Cost is for core staff to provide consumables for 1 assay (plate) and instrument time.	Inquire
Seahorse XFe96 Flux Analyzer - full service	Seahorse assay (ATP production rate, glycolytic rate, etc.). Cost is for instrument time + consumables (1 assay) + labor (provided by core facility staff).	Inquire
Full Service Experiment - Setup and Sample Preparation		Inquire
▼ ► Data Analysis (2)
Name	Description	Price
Advanced Data Analysis (per hour)		Inquire
Data Analysis (External Data e.g., genomics, RPPA, microarray, sequencing, drug combination effects, etc; per hour)		Inquire
▼ ► Equipment Usage (3)
Name	Description	Price
ProteinSimple Peggy Sue Nanoimmunoassay (size or charge) - Instrument Use	Walk-up access to Peggy Sue for trained users who provide their own reagents and labor. Price is for single-day use. For full service nanoimmunoassays, see Proteomic Services menu.	Inquire
Seahorse XFe96 Flux Analyzer - Instrument Use	Walk-up access to Seahorse XFe96 Flux Analyzer for trained users who provide their own reagents and labor. Cost is for single-day use. For full service Seahorse XFe96 Flux Analysis, see Metabolomic Services menu.	Inquire
Equipment Usage (Metabolomics)	Fee is Per Hour with a minimum usage of 1 hour	Inquire
▼ ► NULISA (2)
Name	Description	Price
NULISA - Inflammation Panel (human)	250 cytokines, chemokines, inflammation markers measured with ultra-high sensitivity	Inquire
Patient Sample Aliquot	Aliquot patient liquid samples to avoid future freeze/thaw for clients. Price is for one sample (one tube of original sample).	Inquire
▼ ► dPCR (1)
Name	Description	Price
digital PCR-instrument time and consumables (per plate of up to 16 samples)	Applied Biosystems (Thermo) Absolute Q digital PCR system. Cost is for instrument time and consumables for 1 plate (16 wells). Please bring your own DNA and primers/TaqMan probes.	Non-Academic $598.69 each Internal $300.00 each External in network $319.15 each External out of network $517.02 each UT-System $319.15 each

DO NOT SUBMIT SAMPLES WITH PATIENT HEALTH INFORMATION. This includes medical record numbers or pathology file identifiers.