In order to continue obtaining Core support in a challenging environment, it is crucial that our users acknowledge the following grants in all publications (see following text). Thank you!
“This work was supported in part by Cancer Prevention Research Institute of Texas (CPRIT) grant number RP130397 and NIH grant numbers 1S10OD012304-01 and P30CA016672.”
Mission. The mission of the Metabolomics Core Facility is to provide the research community with cutting-edge expertise and services for sensitive, precise, and accurate measurement of metabolites using mass spectrometry-based technologies.
Metabolomics:
Targeted
Non-targeted
Other Services
Data Analysis:
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AMINO ACIDS:
1 | 4-hydroxy-proline | 16 | isoleucine (Ile) |
2 | acetylcholine | 17 | leucine (Leu) |
3 | alanine (Ala) | 18 | lysine (Lys) |
4 | arginine (Arg) | 19 | methionine (Met) |
5 | asparagine (Asn) | 20 | S-adenosylhomocysteine (SAH) |
6 | carnitine | 21 | ornithine |
7 | citrulline | 22 | phenylalanine (Phe) |
8 | creatine | 23 | proline (Pro) |
9 | creatinine | 24 | sarcosine |
10 | cysteine (Cys) | 25 | serine (Ser) |
11 | cystine (Cys-Cys disulfide) | 26 | taurine |
12 | gamma-aminobutyric acid (GABA) | 27 | threonine (Thr) |
13 | glutamine (Gln) | 28 | tryptophan (Trp) |
14 | glycine (Gly) | 29 | tyrosine (Tyr) |
15 | histidine (His) | 30 | valine (Val) |
ORGANIC ACIDS:
1 | glutamate (Glu) |
2 | glutamine (Gln) |
3 | aspartate (Asp) |
4 | alpha-ketoglutarate (a-KG) |
5 | 2-hydroxyglutarate (2-HG) |
6 | pyruvate |
7 | lactate |
8 | pyroglutamic acid (pGlu) |
POLYAMINES:
1 | tyramine | 8 | putrescine |
2 | tryptamine | 9 | agmatine |
3 | N-acetylputrescine | 10 | hypusine |
4 | diacetylspermine | 11 | diaminopropane |
5 | ornithine | 12 | N-acetylspermine |
6 | diaminohexane | 13 | spermidine |
7 | cadaverine | 14 | spermine |
DNA DAMAGE METABOLITES:
1 | NAD(+) |
2 | AMP |
3 | NADH |
4 | ADP-ribose |
5 | ADP |
6 | ATP |
Faculty Oversight:
Dr. Mien-Chie Hung
Dr. John Weinstein
Metabolomics Services:
Phil Lorenzi, Ph.D., Co-Director
Email: PLLorenzi@mdanderson.org
713.792.9999
Hours: | Location: |
Sample Drop Off: Monday - Friday 7 AM - 5 PM By appointment only! |
Lab: 2SCR3.3217 Phil Lorenzi: 2SCR3.3029 |
Our website contains additional information including an FAQ and tutorials.
Name | Role | Phone | Location | |
---|---|---|---|---|
Phil Lorenzi, Ph.D |
Co-Director
|
713-792-9999
|
PLLorenzi@mdanderson.org
|
2SCR3.3029
|
Services & Rates |
► Metabolomics (17) | |||
Name | Description | Price | |
---|---|---|---|
New Assay Setup (Per Day) |
Don't see the molecule you want to measure listed on our menu? We can probably add it to one of our existing assays or build a new method. Most molecules can be added with 2-3 days of labor, which includes optimization of a range of parameters including extraction/recovery. Challenging biological matrices (e.g., stool) often require more time for optimization. |
Inquire | |
Non-targeted Profiling (metabolites + lipids) |
Includes profiling by IC-MS (polar metabolomics), HILIC-MS (polar metabolomics), and C30-MS (lipidomics). |
Inquire | |
Lipidomic Profiling | Inquire | ||
IC-MS Anionic Metabolites (Targeted) |
Approximately 150 anionic metabolites including glycolysis, TCA cycle, pentose phosphate pathway, and nucleotides. |
Inquire | |
Fatty Acids, Medium- and Long-Chain, Free | Inquire | ||
Fatty Acids, Medium- and Long-Chain, Total | Inquire | ||
Amino Acids (Targeted) |
Approximately 30 amino acids and related metabolites. |
Inquire | |
Polyamines (Targeted) |
Approximately 14 polyamines. |
Inquire | |
CoAs |
Optimized method for the measurement of 3 coenzyme A (CoA) species. |
Inquire | |
L-, D-2-HG |
Chiral separation and quantitation of L- and D-2-hydroxyglutarate by LC-MS/MS. |
Inquire | |
Bile Acids (Targeted) |
Targeted assay for the measurement of 18 bile acids |
Inquire | |
Stable Isotope Tracer Analysis (Targeted) | Inquire | ||
Stable Isotope Tracer Analysis (Non-targeted) | Inquire | ||
Targeted analysis (custom) | Inquire | ||
Seahorse XFe96 Flux Analyzer - instrument time and consumables |
Seahorse assay (ATP production rate, glycolytic rate, etc.). Cost is for core staff to provide consumables for 1 assay (plate) and instrument time. |
Inquire | |
Seahorse XFe96 Flux Analyzer - full service |
Seahorse assay (ATP production rate, glycolytic rate, etc.). Cost is for instrument time + consumables (1 assay) + labor (provided by core facility staff). |
Inquire | |
Full Service Experiment - Setup and Sample Preparation | Inquire | ||
► Data Analysis (2) | |||
Name | Description | Price | |
Data Analysis (Internal Data, per hour) | Inquire | ||
Data Analysis (External Data e.g., genomics, RPPA, microarray, sequencing, drug combination effects, etc; per hour) | Inquire | ||
► Equipment Usage (3) | |||
Name | Description | Price | |
ProteinSimple Peggy Sue Nanoimmunoassay (size or charge) - Instrument Use |
Walk-up access to Peggy Sue for trained users who provide their own reagents and labor. Price is for single-day use. For full service nanoimmunoassays, see Proteomic Services menu. |
Inquire | |
Seahorse XFe96 Flux Analyzer - Instrument Use |
Walk-up access to Seahorse XFe96 Flux Analyzer for trained users who provide their own reagents and labor. Cost is for single-day use. For full service Seahorse XFe96 Flux Analysis, see Metabolomic Services menu. |
Inquire | |
Equipment Usage (Metabolomics) |
Fee is Per Hour with a minimum usage of 1 hour |
Inquire |