MDACC Proteomics and Metabolomics

Please Acknowledge

In order to continue obtaining Core support in a challenging environment, it is crucial that our users acknowledge the following grants in all publications (see following text).  Thank you!


This work was supported in part by Cancer Prevention Research Institute of Texas (CPRIT) grant number RP130397 and NIH grant number 1S10OD012304-01.


Overview of Services

Mission.  The mission of the Proteomics and Metabolomics Core Facility is to provide the research community with cutting-edge expertise and services for sensitive, precise, and accurate measurement of proteins and metabolites using mass spectrometry-based technologies.





  • Protein Identification: High sensitivity (silver stain OK), up to 100 proteins or more may be indentifiable. 
  • Molecular Weight Determination: Check the MW of your peptide or molecule.  Proteins may be measured if enough clean protein is available (larger proteins are less reliable). 
  • Quantitative Protein Analysis:  Using stable isotope methods, iTRAQ, etc. For SRM/MRM methods on our tandem quadrupole MS, please inquire. Quantitative Protein Profiling using MRM, HRM or DIA (Swath-like) methods, with or without reference peptides (lists available, custom lists can be built).
  • Posttranslational Modification Analysis:  Phosphorylation, acetylation, methylation, etc. Please inquire for more detailed information.
  • LC-MS Analysis:  Size exclusion chromatography and simple LC-MS.  Please inquire for more detailed information and other chromatographic analyses available.




  • Amino Acids (30): Absolute quantitation of the 18 proteogenic amino acids and 12 related molecules. (See list below)
  • Organic Acids (8): Absolute quantitation of 8 organic acids. (See list below)
  • Glutathione (2): Absolute quantitation of reduced (GSH) and oxidized (GSSG) glutathione.
  • Polyamines (14): Absolute quantitation of 14 polyamines. (See list below)
  • DNA Damage Metabolites (6): Absolute quantitation of 6 DNA damage metabolites. (See list below)
  • Anionic Metabolites by IC-MS (80): Relative quantitation of metabolites associated primarily with glycolysis, TCA cycle, and pentose phosphate pathway.
  • Stable Isotope Tracer Analysis: Measurement of stable isotope incorporation into targeted metabolites (assays above) downstream of a chosen tracer metabolite. Multiplexed, multi-tracer analysis is available.



  • Profiling (complete): Relative quantitation of metabolites from multiple fractions.
  • Lipidomic Profiling: Relative quantitation of multiple classes of lipid metabolites from nonpolar fractions.
  • Hyaluronic Acid Profiling: Relative quantitation of hyaluronic acid oligomers.
  • Tracer Analysis: Measurement of stable isotope incorporation into non-targeted metabolites downstream of a chosen tracer metabolite.  Multiplexed, multi-tracer analysis is available.


     Other Services

  • Redox Metabolism Assays: Relative quantitation of ROS (H2O2) using a microplate (96-well) assay.
  • Mechanism of Action Studies: Assistance with pharmacological experiments to determine an agent's mechanism of action by integrating pharmacological endpoints (e.g., cell viability) with metabolomic and other omic data.
  • Sample Preparation Assistance: Assistance with or full-service provision of sample preparation. For example, you can ship cells and treatment(s), we can work together to design an experiment, and we can conduct the entire experiment.


Data Analysis:

  • Data Analysis: Many of the reports generated by our facility are time-consuming to assemble; we add a data analysis fee of $250 for reports that require 1-4 h of effort. Expertise includes bioinformatics, statistics, pathway analysis, and/or assistance preparing materials for manuscripts or presentations. Many data types accepted (genomic, proteomic, metabolomic, pharmacological, etc.).
  • Drug Combination Analysis: We have developed software for conducting 3-D response surface and response envelope analyses of drug combinations for the statistical determination of synergy, additivity, or antagonism. (Please contact Phil Lorenzi to discuss)



1 4-hydroxy-proline 16 isoleucine (Ile)
2 acetylcholine 17 leucine (Leu)
3 alanine (Ala) 18 lysine (Lys)
4 arginine (Arg) 19 methionine (Met)
5 asparagine (Asn) 20 S-adenosylhomocysteine (SAH)
6 carnitine 21 ornithine
7 citrulline 22 phenylalanine (Phe)
8 creatine 23 proline (Pro)
9 creatinine 24 sarcosine
10 cysteine (Cys) 25 serine (Ser)
11 cystine (Cys-Cys disulfide) 26 taurine
12 gamma-aminobutyric acid (GABA) 27 threonine (Thr)
13 glutamine (Gln) 28 tryptophan (Trp)
14 glycine (Gly) 29 tyrosine (Tyr)
15 histidine (His) 30 valine (Val)



1 glutamate (Glu)
2 glutamine (Gln)
3 aspartate (Asp)
4 alpha-ketoglutarate (a-KG)
5 2-hydroxyglutarate (2-HG)
6 pyruvate
7 lactate
8 pyroglutamic acid (pGlu)



1 tyramine 8 putrescine
2 tryptamine 9 agmatine
3 N-acetylputrescine 10 hypusine
4 diacetylspermine 11 diaminopropane
5 ornithine 12 N-acetylspermine
6 diaminohexane 13 spermidine
7 cadaverine 14 spermine



1 NAD(+)
4 ADP-ribose



Faculty Oversight:

Dr. Gordon Mills

Dr. John Weinstein


Proteomic Services:

David Hawke, Ph.D., Director




Metabolomics Services:

Phil Lorenzi, Ph.D., Co-Director




Location and hours of operation

Hours: Location:

Sample Drop Off:

Monday - Friday

7 AM - 5 PM

By appointment only!                        

Lab: 2SCR3.3123
David Hawke: 2SCR3.3025             
Phil Lorenzi: 2SCR3.3029


Links and Resources

Our website contains additional information including an FAQ and tutorials.

  1. Proteomics and Metabolomics Core Website


Name Role Phone Email Location
David Hawke, Ph.D
Phil Lorenzi, Ph.D

Services & Rates

Search available services: View: by category alphabetically
Data Analysis (3)
Equipment Usage (2)
Metabolomics (13)
Proteomics (5)

Members at UT Proteomics

University of Texas Medical Branch - Galveston

Core Name Primary Contact Email Phone Number/Ext
Biomolecular Resource Facility

William Russell, Ph.D (409) 772-3579
Core Name Primary Contact Email Phone Number/Ext
UTHSA Mass Spectrometry Laboratory

The lab has multiple modern mass spectrometers. Protein analysis services include molecular mass determination, protein identification, sequence and post-translational modification site characterization. Quantitative analysis of lipids and intermediary metabolites by GC/MS is also available.

Susan Weintraub, Ph.D. (210) 567-4043
Core Name Primary Contact Email Phone Number/Ext
MDACC NORTH Campus Flow Cytometry and Cellular Imaging Core Facility

The Flow Cytometry and Cellular Imaging Facility was established to provide access to state of the art cell analysis technology for researchers, and provides cell sorting, analytical flow cytometry, cellular imaging and custom monoclonal antibody mAb conjugations to its users.

Joan Hoover-Zuniga 713-745-1749
MDACC Proteomics and Metabolomics

The mission of the Proteomics and Metabolomics Core Facility is to provide the research community with cutting edge expertise and services for sensitive, precise, and accurate measurement of proteins and metabolites using mass spectrometrybased technologies.p</p>

David Hawke, Ph.D 713-834-6096
MDACC RPPA-Functional Proteomics

Functional Proteomics Reverse Phase Protein Array RPPA Core provides cancer center members with access to a powerful, highthroughput, quantitative costeffective antibodybased assay to characterize basal or ligandinduced protein expression and modification, and timeresolved responses appropriat

Kathryn Aziz 713-792-5743

University of Texas at Austin

Core Name Primary Contact Email Phone Number/Ext
Core Name Primary Contact Email Phone Number/Ext
UTHealth Proteomics Service Center

The CTPC services provided are designed to identify and quantitate proteins and their modifications in a broad range of research specimens from simple purified protein samples to biomarker discovery and verification in complex mixtures such as cell and tissue extracts, plasma andor other biofluids

Li Li 713-500-2456

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