MDACC Proteomics and Metabolomics

Please Acknowledge

In order to continue obtaining Core support in a challenging environment, it is crucial that our users acknowledge the following grants in all publications (see following text).  Thank you!

 

This work was supported in part by Cancer Prevention Research Institute of Texas (CPRIT) grant number RP130397 and NIH grant number 1S10OD012304-01.

 

Overview of Services

Mission.  The mission of the Proteomics and Metabolomics Core Facility is to provide the research community with cutting-edge expertise and services for sensitive, precise, and accurate measurement of proteins and metabolites using mass spectrometry-based technologies.

 

SERVICES

 

Proteomics:

  • Protein Identification: High sensitivity (silver stain OK), up to 100 proteins or more may be indentifiable. 
  • Molecular Weight Determination: Check the MW of your peptide or molecule.  Proteins may be measured if enough clean protein is available (larger proteins are less reliable). 
  • Quantitative Protein Analysis:  Using stable isotope methods, iTRAQ, etc. For SRM/MRM methods on our tandem quadrupole MS, please inquire. Quantitative Protein Profiling using MRM, HRM or DIA (Swath-like) methods, with or without reference peptides (lists available, custom lists can be built).
  • Posttranslational Modification Analysis:  Phosphorylation, acetylation, methylation, etc. Please inquire for more detailed information.
  • LC-MS Analysis:  Size exclusion chromatography and simple LC-MS.  Please inquire for more detailed information and other chromatographic analyses available.

 


Metabolomics: 

  • Targeted
  • Amino Acids (30): Quantitative LC-MS assay for measurement of the 18 proteogenic amino acids and 12 related molecules. (See List Below)
  • Organic Acids (8): Quantitative LC-MS assay for measurement of 8 organic acids. (See List Below)
  • Glutathione (2): Quantitative LC-MS assay for measurement of reduced (GSH) and oxidized (GSSG) glutathione. (See List Below)
  • Polyamines (14): Quantitative LC-MS assay for measurement of 14 polyamines (See List Below)
  • DNA Damage Metabolites (6): Quantitative LC-MS assay for measurement of 6 DNA damage metabolites (See List Below)

 

  • Non-Targeted
  • Profiling: Quantitative LC-MS assay for measurement of metabolites in non-targeted fashion; multiple configurations available.
  • Tracer Analysis: Quantitative (ratiometric) LC-MS assay for measurement of stable isotope label incorporation into non-targeted metabolites.

 

Additional Data Analysis:

  • Additional Data Analysis: Bioinformatics, statistics, pathway analysis, and/or assistance preparing materials for manuscripts or presentations. (For data from within the Proteomics/Metabolomics Core (Internal) and other types of data from outside this Core (External), i.e. Genomics, RPPA, Microarray, Sequencing, etc...)
  • Drug Combination Analysis: 3-dimensional surface response analysis of drug combinations incorporating multiple additivity models for the statistical determination of synergy, additivity, or antagonism (Please contact Phil Lorenzi to discuss prior to submitting data for this analysis)

____________________________________________________________________________________________________________________________________________________________________________________________________________________________

 AMINO ACIDS: 

1 4-hydroxy-proline 16 isoleucine (Ile)
2 acetylcholine 17 leucine (Leu)
3 alanine (Ala) 18 lysine (Lys)
4 arginine (Arg) 19 methionine (Met)
5 asparagine (Asn) 20 S-adenosylhomocysteine (SAH)
6 carnitine 21 ornithine
7 citrulline 22 phenylalanine (Phe)
8 creatine 23 proline (Pro)
9 creatinine 24 sarcosine
10 cysteine (Cys) 25 serine (Ser)
11 cystine (Cys-Cys disulfide) 26 taurine
12 gamma-aminobutyric acid (GABA) 27 threonine (Thr)
13 glutamine (Gln) 28 tryptophan (Trp)
14 glycine (Gly) 29 tyrosine (Tyr)
15 histidine (His) 30 valine (Val)

  

ORGANIC ACIDS: 

1 glutamate (Glu)
2 glutamine (Gln)
3 aspartate (Asp)
4 alpha-ketoglutarate (a-KG)
5 2-hydroxyglutarate (2-HG)
6 pyruvate
7 lactate
8 pyroglutamic acid (pGlu)

  

GLUTATHIONE:

1 glutathione, reduced (GSH)
2 glutathione, oxidized (GSSG)

 

POLYAMINES:

1 tyramine 8 putrescine
2 tryptamine 9 agmatine
3 N-acetylputrescine 10 hypusine
4 diacetylspermine 11 diaminopropane
5 ornithine 12 N-acetylspermine
6 diaminohexane 13 spermidine
7 cadaverine 14 spermine

 

DNA DAMAGE METABOLITES:

1 NAD(+)
2 AMP
3 NADH
4 ADP-ribose
5 ADP
6 ATP


Leadership 

 

Faculty Oversight:

Dr. Gordon Mills

Dr. John Weinstein

 

Proteomic Services:

David Hawke, Ph.D., Director

Email:  dhawke@mdanderson.org

713.834.6096

 

Metabolomics Services:

Phil Lorenzi, Ph.D., Co-Director

Email: PLLorenzi@mdanderson.org

713.792.9999

 

Location and hours of operation

Hours: Location:

Sample Drop Off:

Monday - Friday

7 AM - 5 PM

By appointment only!                        

Lab: 2SCR3.3123
David Hawke: 2SCR3.3025             
Phil Lorenzi: 2SCR3.3029

  

Links and Resources

Our website contains additional information including an FAQ and tutorials.

  1. Proteomics and Metabolomics Core Website

Contacts

Name Role Phone Email Location
David Hawke, Ph.D
Director
 
713-834-6096
 
dhawke@mdanderson.org
 
2SCR3.3025
 
Phil Lorenzi, Ph.D
Co-Director
 
713-792-9999
 
PLLorenzi@mdanderson.org
 
2SCR3.3029
 

Services

Name Description
Proteomics and Metabolomics Requests 

 

First time users MUST have a consultation regarding experimental design prior to submitting samples.

  • For Proteomics: Please contact David Hawke at dhawke@mdanderson.org to schedule a consultation.
  • For Metabolomics: Please contact Phil Lorenzi at pllorenzi@mdanderson.org to schedule a consultation.

 

 **All pricing is including the 60% overhead fees if you are an External:Out-of-Network or Non-academic Customer.**

 

**SAMPLE SUBMISSION IS BY APPOINTMENT ONLY (AFTER APPROVAL OF YOUR REQUEST)**

 
Additional Data Analysis (Internal Data--generated from Proteomics/Metabolomics Core)   
Equipment Usage (Proteomics) 

 

Fee is Per Hour with a minimum usage of 1 hour

 
New Assay Setup   
Protein Identification   
Additional Data Analysis (External Data--generated from other sources, i.e. genomics, RPPA, microarray, sequencing, etc)   
Equipment Usage (Metabolomics) 

Fee is Per Hour with a minimum usage of 1 hour

 
Molecular Weight Determination   
Sample Preparation Assistance   
(30) Amino Acids (Targeted)   
Drug Combination Analysis    
Post-translational Modification Analysis   
(8) Organic Acids (Targeted)   
LC or LC-MS Analysis   
(2) Glutathione (Targeted)   
Quantitative Analysis (Proteins Setup)   
(14) Polyamines (Targeted)   
(6) DNA Damage Metabolites (Targeted)   
Profiling (Non-targeted)   
Tracer Analysis (Non-targeted)   

Services & Rates


Search available services: View: by category alphabetically
Additional Data Analysis (3)
Equipment Usage (2)
Metabolomics (9)
Proteomics (5)


Members at UT Proteomics

University of Texas Medical Branch - Galveston

Core Name Primary Contact Email Phone Number/Ext
Biomolecular Resource Facility

William Russell, Ph.D bill.russell@utmb.edu (409) 772-3579
Core Name Primary Contact Email Phone Number/Ext
UTHSA Mass Spectrometry Laboratory

Susan Weintraub, Ph.D. weintraub@uthscsa.edu (210) 567-4043
Core Name Primary Contact Email Phone Number/Ext
MDACC NORTH Campus Flow Cytometry and Cellular Imaging Core Facility

The Flow Cytometry and Cellular Imaging Facility was established to provide access to state of the art cell analysis technology for researchers, and provides cell sorting, analytical flow cytometry, cellular imaging and custom monoclonal antibody mAb conjugations to its users.

Joan Hoover-Zuniga jezuniga@mdanderson.org 713-745-1749
MDACC Proteomics and Metabolomics

The mission of the Proteomics and Metabolomics Core Facility is to provide the research community with cutting edge expertise and services for sensitive, precise, and accurate measurement of proteins and metabolites using mass spectrometrybased technologies.p</p>

David Hawke, Ph.D dhawke@mdanderson.org 713-834-6096
MDACC RPPA-Functional Proteomics

Functional Proteomics Reverse Phase Protein Array RPPA Core provides cancer center members with access to a powerful, highthroughput, quantitative costeffective antibodybased assay to characterize basal or ligandinduced protein expression and modification, and timeresolved responses appropriat

Kathryn Aziz ccsgrppa@mdanderson.org 713-792-5743

University of Texas at Austin

Core Name Primary Contact Email Phone Number/Ext
Core Name Primary Contact Email Phone Number/Ext
UTHealth Proteomics Service Center

The CTPC services provided are designed to identify and quantitate proteins and their modifications in a broad range of research specimens from simple purified protein samples to biomarker discovery and verification in complex mixtures such as cell and tissue extracts, plasma andor other biofluids

Li Li Li.Li@uth.tmc.edu 713-500-2456